Entering edit mode
1 day ago
chrisgr
▴
20
I'm trying a crisprscreen analysis using Mageck. I have both pre-treatment(baseline) ánd WT samples (and ofcourse treatment samples). Online I have only found people simply comparing the WT vs treatment. But isn't there a way to include the pre-treatment samples to the command of Mageck in order to normalise the WT and treatment with a baseline first? Or what would be the best approach?
My current command comparing pre-treat vs WT (not including the treatment)
mageck test -k GSE277685_sgRNA_count_table.txt -t Input1_rep1,Input1_rep2 -c WT_week6_rep1,WT_week6_rep2, -n mageck_output/WT/WT_week6
Hope anyone could guide me a bit here
All the best,
Chris
You will want to use
mageck mle
analysis for that.