Mitochondrial Transcriptome Analysis with PacBio RNA Data using Geneious Prime

Question

[hashim.rana11]

Dear experts and researchers,

I am working on mitochondrial transcriptomics using RNA PacBio data. Using IsoSeq3 and Minimap2, I successfully created BAM and BAI files. I have now uploaded these files, along with the reference genome, into Geneious Prime® 2024.0.7 for analysis.

My goal is to perform detailed mitochondrial transcriptome analysis. However, I am confused about the next steps and whether I am proceeding correctly.

Could someone please guide me on how to proceed further with the analysis in Geneious Prime after uploading the BAM and BAI files? Any advice would be greatly appreciated.

Thank you in advance for your help!

[GenoMax]

It would be best to contact Geneious Prime support for immediate help. It is licensed software and not many from Biostar audience will have access.