weird entry in EnhanceVolcano Volcano plot -RNAseq

Question

[Yogesh]

Hi folks,

I am trying to plot volcano for one of my data. but i am getting some weird entry in one of the contrast analysis. can anyone help me understand why is this happening? Attaching the figure here. I have already checked my rownames, they are just fine. Plot Image is attached herewith

[MRezaei]

Hi. Could you share the script?

Answer 1

[weishengxin]

It's possible that the delimiter was not specified, which has resulted in the gene names becoming a combination of the name and a series of counts. You need to specify the delimiter, for example, when reading the input file, add sep=",", and also specify the columns required for plotting, which are typically three columns: name, log2fc, p or fdr.