error in DESeq2 Determines differentially expressed features from count tables

Question

[razieamiri.1997]

I have faced errors in (DESeq2 Determines differentially expressed features from count tables) like this:

primary factor: donor48_donor63
Error in
DESeqDataSetFromMatrix(countData = tbl, colData = subset(sample_table
ncol(countData) == nrow(colData) is not TRUE
Calls: get_deseq_dataset ->DESeqDataSetFromMatrix -> stopifnot

How can I unwind?

[1769mkc]

add your dataframe like small subset you can use dput(df(head) along with the metadata you are trying

[i.sudbery]

The error message is telling you that the the number of columns in the count data is not the same as the number of rows in the sample data. Since each column in the count data should be a sample, and each row in the sample data should be a sample, these should be the same.