Your suggestion how to annotate cell type from visium data
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4 weeks ago
Chris ▴ 340

Hi all,

I try to annotate cell type from visium data (brain tissue) but don't know what went wrong. I followed the method here that uses a snRNA-seq dataset as reference.

However, most of cell types from SpatialFeaturePlot() had zero score. Would you please have a suggestion? I found another method "Annotation of spatially resolved single-cell data with STELLAR" but I think seurat is the most popular. Can't upload image (image:Exceeded the maximum amount of images you can upload.) Thank you so much!

Update. Trying cell2location, hope it will work.

spatial-transcriptomics • 152 views
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