What is a genomic range that would almost always get decent WGS coverage but is never covered by WES?
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21 days ago

So some intergenic range that isn't hard to sequence and is nowhere near the periphery of a gene that would be on an exome kit.

wes wgs • 717 views
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What would you want to do with such a region(s)?

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use it to know if I was looking at wgs or wes

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You could take BED regions from some of the common WES kits and then create a "superset" region file to subtract from the genome (and perhaps remove blacklisted regions as well).

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20 days ago

What about some of the well known enhancer regions, the globin locus LCR? Likely to be highly sequenceable, and yet not on exome panels.

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21 days ago

I don't really understand this question?

Any intron is going to get good relative coverage in WGS over WES... Anything over 5 kb (probably even 1 kb) away from a targeted exon will have 0 reads (in WES) - outside of background reads... so like avoid regions with transposon/repeat elements. If you want to go over the top best is probably an intron with high conservation.

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i think you did understand the question, i just wanted a chr:start-end region to use as a litmus test.

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