Repeat CT in overrepresented sequences
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7 weeks ago
Analeigh • 0

I'm working on an scRNA-seq project and fastqc keeps identifying overrepresented sequences consisting of C and T.

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I can’t make sense on where this could come from. Any ideas? Thanks!

ScRNA fastqc overrepresented sequences • 324 views
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fastqc keeps identifying overrepresented sequences consisting of C and T.

Which read is it located in? fastqc is of limited utility with single cell data (especially 10x).

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CT-rich regions in introns have been reported before. At what positions in your reads does it appear? How does it affect their mapping to reference?

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