Hi,
I have the type of samples below (image).
I want to compare Type A and Type B in Stage1 under untreated and treated conditions: for example:
Type A vs Type B Stage 1 (untreated vs treated)
Samples used:
Type A_Stage1_untreated
Type B_Stage2_treated
Is this comparison possible in differential expression analysis? In addition, how will I handle one replicate in differential expression analysis? Thank you!