m6A modification prediction using Nanopore DRS data (RNA004)

Question

[baibhu1234]

Hi everyone, I was doing m6A site modification prediction using m6Anet in a plant species. m6Anet have two models for RNA002 chemistry for human and arabidopsis.

but recently they have updated the m6net even for RNA004 chemistry. but the model is trained only on human cell line data.

which model i should use for predicting m6A sites in my plant? should it be RNA004 model trained with human data or RNA002 model trained with arabidopsis data.

[GenoMax]

If you have the complete data folder you should consider using dorado provided by ONT to do the basecalling. dorado should pick the correct model applicable for your data as long as there is direct internet access.