vcf files comparison to find unique variants

Question

[Nesma]

I have 2 vcf files for a single sample that has been sequenced twice, once before and again after developing resistance to a certain antibiotic. Is there a command I can use to extract only newly developed variants in the (post resistance) sample? (I mean to exclude mutual variants in both files)

[Bastien Hervé]

This has been asked before : Best way to compare two samples in a VCF file

Answer 1

[GenoMax]

Use bcftools isec as explained here: How bcftools isec works ?