how to choose cufflinks library type from infer_experiment
0
0
Entering edit mode
25 days ago
sgupt46 • 0

Hi, Could someone help me identify the input parameter for cufflinks based on the output of infer_experiemnts.py

This is PairEnd Data
Fraction of reads failed to determine: 0.0896
Fraction of reads explained by "1++,1--,2+-,2-+": 0.0123
Fraction of reads explained by "1+-,1-+,2++,2--": 0.8981

What would be the library type for cufflinks out of the suggested ones

Supported library types:
    ff-firststrand
    ff-secondstrand
    ff-unstranded
    fr-firststrand
    fr-secondstrand
    fr-unstranded (default)
    transfrags

Another dataset has strand info like this, what would be the library type for it?

Fraction of reads failed to determine: 0.0801
Fraction of reads explained by "1++,1--,2+-,2-+": 0.3720
Fraction of reads explained by "1+-,1-+,2++,2--": 0.5479
RSeQC rnaseq cufflinks • 304 views
ADD COMMENT
0
Entering edit mode

See: RSeQC Output from infer_experiment.py - what does it mean?

In second case that looks like an unstranded library.

ADD REPLY
0
Entering edit mode

So, for the first case the answer would be fr-firststrand, while for the second it would be fr-unstranded?

ADD REPLY
1
Entering edit mode

That is correct.

ADD REPLY

Login before adding your answer.

Traffic: 1841 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6