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RNA-Sequencing, paired end reads, have aligned BAM files, and want to to use feature counts, but cannot find a gtf file for adenovirus 5 anywhere. I have checked viral gene assemblies and have reference genome/fasta file but nothing for annotation. Any ideas please?
Thank you - I didn't see it so I clearly need to step away from the screen for a while.
A related question - I work with an intracellular human virus, and my genome is a custom genome consisting of Hg38+ virus (concatenated). I need a merged GTF file for the two for annotation (RNA-Sequencing).
What is the best way to merge two unrelated GTF files (that is NOT Agat), I cannot install Agat......