Entering edit mode
12 days ago
zizigolu
★
4.3k
Hi
How can I get fasta sequence for gene body DLK1
plus to 10 kb of its upstream?
Hi
How can I get fasta sequence for gene body DLK1
plus to 10 kb of its upstream?
Getting the gene sequence can be a little more complicated than one might assume.
The word "gene" is burdened with many meanings.
Are you looking for coding regions? Transcripts?
Upstream of what? Start codon of a transcript, transcription start site? What if the gene has multiple transcript start sites?
There are many resources that can give you answers, but first and most important element is to define what you are really after.
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bedtools getfasta
. You're an experienced user who has been called out for laziness many! times before, this is yet another example of zero effort.Use
BioMart
orucsc table browser
(LINK).