comparing STARsolo UMI-based directional deduplication vs umi-tools?

Question

[mk]

According to the STARsolo docs, setting --soloUMIdedup 1MM_Directional_UMItools:

follows the "directional" method from the UMI-tools

Can anyone confirm how this differs from calling umi-tools dedup like:

# Note that by default, `--method=directional` for umi-tools.

umi_tools dedup --per-cell --extract-umi-method=tag --umi-tag=UR --cell-tag=CR \
-I $input_bam \
-S $output_bam;

Answer 1

[i.sudbery]

I haven't done extensive benchmarking, but the claim from the STARsolo authors is that it should be the same.