Entering edit mode
14 days ago
Apprentice
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170
I have dataset of scRNA-seq data measured at 10x for 4 patients and 4 healthy individuals. I would like to merge these eight scRNA-seq data and perform hdWGCNA. I have 8 h5-format files generated by CellRanger for 8 people's scRNA-seq data, so I first created a seurat object with the IntegrateData function in Seurat R package. However, I don't know what to do next. Should I then implement the IntegrateLayers or JoinLayers function before implementing hdWGCNA? I would appreciate your guidance. Or, if you have any pipelines or tutorials that show how to perform hdWGCNA on h5 files that contain multiple samples, please let me know.