Deleted:How should I integrate the h5 files of multiple samples in order to implement hdWGCNA?
0
0
Entering edit mode
14 days ago
Apprentice ▴ 170

I have dataset of scRNA-seq data measured at 10x for 4 patients and 4 healthy individuals. I would like to merge these eight scRNA-seq data and perform hdWGCNA. I have 8 h5-format files generated by CellRanger for 8 people's scRNA-seq data, so I first created a seurat object with the IntegrateData function in Seurat R package. However, I don't know what to do next. Should I then implement the IntegrateLayers or JoinLayers function before implementing hdWGCNA? I would appreciate your guidance. Or, if you have any pipelines or tutorials that show how to perform hdWGCNA on h5 files that contain multiple samples, please let me know.

Seurat hdWGCNA scRNA-seq • 184 views
ADD COMMENT
This thread is not open. No new answers may be added
Traffic: 2061 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6