Entering edit mode
6 days ago
María José
▴
10
Hi,
I would like to use minimap2 for analyzing Nanopore and PacBio data. Depending on the version, one of ax options is recommended. Ex: PacBio CLR genomic reads -ax map-pb
. While many research papers detail the methodology of library preparations and kits used. It didn't specify, for example, whether PacBio reads are CLR or Hifi. How could I retrieve this kind of information?
I would think that
map-pb
should cover all reads from PacBio as a technology because those reads will have a certain error profile as far asminimap2
goes.https://www.pacb.com/blog/hifi-difference-not-being-clr/ should help clarify.