Alluvial Plot of TCR Sequencing Data

Question

[ab39]

Hi everyone,

I'm new to the field of bioinformatics and am currently trying to create an alluvial plot using my TCR sequencing data, but I'm having some difficulties.

First, I’m struggling to add a black bin to represent clonotypes found in ACTP but not in T0. It seems that alluvial plots always require connections between the two columns, which is making this challenging.

Additionally, the plot has two different y-axes: one for T0 and another for ACTP.

I’m unsure how to achieve this using ggalluvial. Any help or guidance would be greatly appreciated!