Box plot of gene expression data
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10 weeks ago
a.stef.44 ▴ 10

Hello,

I would like to visualize gene expression of significant genes between case/control, but I am not sure which values to use. Count data, vst normalized counts data, or DESeq2 normalized data?

Thank you in advance, Aleksandra

boxplot • 302 views
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It doesn't matter as long as it is normalized to account for differential sequencing depth and library composition. If you have vst then use this, it's usually a good choice. Don't show raw counts, the rest is ok.

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