This is RNA-seq data that I obtained through analysing the raw reads to get the counts matrix, after which DESeq2 differential expression analysis was done and after obtaining the normalized data I plotted the data to visualize the distribution and it gave this multi-peak plot, I am not an expert in RNA-seq data so can anyone interpret this and what might be the problem that led to such distribution? or is it even a problem!? also, can I proceed with such distribution for further analysis or is there a solution to apply to the data?
Note: the thing I doubt is when I was downloading the samples from SRA each sample had multiple runs in it so after a little bit of search I found 2 opinions about dealing with the runs either merging the runs and treat the multiple runs as one sample or treating each run as an individual sample and this is what I did in my case
