Hello everyone, I was working on identification and characterisation of miRNAs using sRNA dataset. I have identified the miRNAs using ShortStack program. I have identified the potential mRNA targets using psRNAtarget program.
Now i need to perform the co-expression analysis of miRNAs and corresponding targets. which normalisation i should perform in order to make the comparison unbiased.
Do i need to perform normalisation for library size, mRNA size, and log2 (to control variance) all?
Any suggestion is appreciated. Thanks