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2 days ago
analyst
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60
Hi,
I have constructed single de novo assembly of two varieties diploid and tetraploid. Later on, variants are called for diploid and tetraploid variety. Now i have two merged vcf files one representing diploid variety and other tetraploid variety. Can I further merge both diploid and tetraploid files into one through bcftools without losing ploidy information? Please guide.
Thankyou
How are you calling tetraploid variants out of interest ?
I used freebayes for variant calling setting --ploidy 4 for tetraploid samples and --ploidy 2 for diploid samples.