Error in importing data from 10x genomics into RStudio

Question

[himanshu0102]

Hello all, I am trying to import an H5 file dataset from 10x Genomics into RStudio:

Gene Expression - Feature / cell matrix HDF5 (raw)

But i get an error: invalid multibyte string at '<89>HDF'

I am not able to import the dataset because of this.

Please could someone advice?

Thank you,

Himan

[GenoMax]

Please post exact code/packages you are using. It is unclear what you are doing at this time.

[1769mkc]

can you cite the data set which one you trying?

[himanshu0102]

I am using the Seurat package for scRNA seq analysis.

I am following this link:

https://biostatsquid.com/scrnaseq-preprocessing-workflow-seurat/

But when i try to import data set into RStudio i get the error:

invalid multibyte string at '<89>HDF'

Because of which i am unable to import data into Rstudio.

Is there any way around this?

Thank you.

[GenoMax]

Are you trying to follow the tutorial you linked? Do you get the error on step 1 that is import data?

[himanshu0102]

Yes, that is correct.

[1769mkc]

to help you and at the same time other can help when you can cite what exactly you had ran the code, the Seurat version R session info etc, please provide them since other people can't make a guess what and how you did. So it would be helpful and beneficial if you can provide the information

[jared.andrews07]

Please learn how to ask a good question. We are unable to help you given the information provided.