Entering edit mode
16 days ago
majorprogeria
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0
I am currently doing a project with 10 rna seq dataset from GEO and i want to do their meta analysis with starting steps like Fastqc, MetaDE, PCA, Cluster, Network Analysis and etc. can anyone recommend a research paper which use a pipeline or set up a pipeline. Also i need help with step like what should be include and what is important - like for FastQC what should i be doing ?
Can you please elaborate what you aim to reveal with your "meta-analysis". Frankly, that you mention meta-analysis and fastqc in the same sentence indicates that you have a limited understanding of the term. Nothing wrong with that, we're here to help, but please sa what the rationale of the analysis is so one can follow-up.
this is an pipeline that i am following "https://nbviewer.org/github/metaOmics/tutorial/blob/master/metaOmics_turtorial.pdf" i could'nt use this pipeline because my dataset werent working on this plus the last update is 8 years ago hence the problem we were facing had no solution and the R used in the MetaOmics is older version than whats been used to this date.
i just wanted a basic pipeline of meta analysis which could help me in biomarker identification.