Plant Microbe Interations

Question

[H@rry]

Hi,

I have few genes identified in fungal species and interested to study the expression of those genes. However, the expression data is not available for this fungi. Now I am confused if I can use plant expression data to study the expression of orthologue genes. Will it be relevant studies.

Thank you in advance

Answer 1

[eddie.im]

As far as i know you can't. Each organism has his own level of expression which is regulated by many factors and differs from organism to organism even if the genes are orthologs. Fungi to Plant is a huge evolutionary leap, and even if they are in symbiosis their expression levels should probably be different.

[H@rry]

Thanks alot for the answers. The level is expression for every organism is different and I totally agree. However, could it be possible alternate to study host pathogen interactions in other way using genes from fungi only. I am interested to show the relationship of genes from fungi in plant somehow.

[eddie.im]

I believe it's not possible. The interaction between plant-pathogen is very complex. The very best you can do is try to find a VERY close organism close to your fungi species and study expression levels on it, then you validate with RT-qPCR on your fungi. Yet it's very risky and the species must be closely related...

[H@rry]

Thank you. Sure, the plant pathogen interactions is complex to study. qPCR would be nice options you suggested but infection of plant with the fungi is quite unclear till now. The closely related species has the same scenario of data available. Moreover, I read about the closely related species to this fungi and they are infecting other plants not mainly I wanted to study.