Where can i find some bacterial RNA-Seq data?

Question

[matija.sosic]

Where can i find some bacterial RNA-Seq data, e.g. of Escherichia Coli?

I would like to analayse single bacteria's transcriptome to see which genes get expressed.

Answer 1

[matted]

I like DNAnexus's search engine for the SRA, where you can make a nice query and drill down to get what you want. You can see any experiment with "Escherichia" in the name that's categorized as whole-transcriptome analysis here.

[matija.sosic]

Thanks, this looks great! Do you maybe know how to find out how long are the reads? I tried first result with 36bp, that seems really short to me. Also, when I use fastq-dump to get .fastq file almost half of each read is 'N'.

[matted]

If you look at the run on the SRA website (e.g. this one), it will tell you the number of reads ("spots") and the total number of bases, and you can calculate the read length. I don't know a way of checking for N's without downloading the files, though.

Answer 2

[PoGibas]

Similar question was asked on Seqanswers - total RNA/transcriptome of E.coli or S.cerevisiae.

See sudhirvarma's answer: "The NCBI Short Read Archive has several E. coli and datasets".

[Istvan Albert]

I going to just paste the link here for reference:

http://www.ncbi.nlm.nih.gov/sra/?term=%22Escherichia+coli%22[orgn%3A__txid562]

Answer 3

[GouthamAtla]

A very good data set is here with different conditions and biological replicates