CuffLinks predicts too long UTR for overlapping genes
0
1
Entering edit mode
10.6 years ago
Ann ★ 2.4k

I'm trying to use RNA-Seq data to update gene annotations for Arabidopsis. To start, I tried running CuffLinks over some RNA-Seq alignments.

However, I'm running into a problem. It looks like CuffLinks is predicting ridiculously long UTRs for genes that overlap on opposite strands. The RNA-Seq data are not strand specific.

Please see this link to an igb snapshot that show an example - https://www.dropbox.com/s/10rtzii2w0usjnv/CuffLinksOverlapping.png

Is there a parameter setting I can change in CuffLinks to stop this behavior?

I looked at the user's guide but couldn't find something that looked relevant to this question.

cufflinks RNA-Seq TAIR10 arabidopsis • 2.5k views
ADD COMMENT

Login before adding your answer.

Traffic: 1604 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6