What preliminary steps should I do before checking the quality of sequence for RNA-seq

Question

[M K]

I have RNAseq data for human and mouse, and I am going to start RNA-seq analysis, my data is single end with 100nt. Is there any preliminary steps should I do before checking the quality of sequence, trim adapter ,and, map the reads to human and mouse genome.

Answer 1

[Devon Ryan]

There's nothing that needs to be done prior to QC/trimming.

Answer 2

[Irsan]

know what the research questions are, why they are relevant, what are possible answers (e.g. more less mean expression of gene x in N samples compared to N other samples) and make sure you have a backup are the only things I can think of