Why am I getting the BLAST option error: file does not match input format type
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10.6 years ago
ankita0007 • 0

COMMAND USED: makeblastdb -in sorted_alu_seq.fasta -dbtype 'nucl' -out alu

BLAST option error: sorted_alu_seq.fasta does not match input format type, default input type is FASTA.

>gi|26791|27053|(249223568)|SINE/Alu|1
GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA
GGCGGGCGGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGACCAACAT
GGAGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGG
CGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCT
TGAACCCGGGAGGCGGAGGTTGCGGTGAGCCGAGATCGCGCCATTGCACT
CCAGCCTGGGCAAC
blast makeblastdb • 5.1k views
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Is this the complete file? There is nothing obviously wrong with this entry and makeblastdb works. Are you probably making a simple mistake, e.g. trying to use the wrong file?

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I second this comment. It is possible that you have 1 or more Fastq sequences in your Fasta file.

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1
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It's likely. In my experience, the program cd-hit is pretty great for fasta validation (in the output we get info if sequences were discarded and what they were). Maybe others could suggest other methods..

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similar error!!

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