Semantics: Gene Discovery, Gene Determination?
1
2
Entering edit mode
13.4 years ago

I came across this paper entitled:
Gene Discovery and Tissue-specific Transcriptome Analysis in Chickpea with Massively Parallel Pyrosequencing and Web Resource Development.
http://www.ncbi.nlm.nih.gov/pubmed/21653784

I would like to ask what is people's opinion on calling de novo transcriptome sequencing and assembly for non-model species a "gene discovery" or "gene determination" study.

Does one discover or determine genes by doing transcriptome sequencing with no reference genome? Are there any better suited alternatives for such term?

transcriptome gene • 2.0k views
ADD COMMENT
1
Entering edit mode
13.4 years ago

A semantics issue, clearly. I would say that providing the initial evidence - be that genomic DNA sequence or transcriptome analysis - for a gene, then that is discovery. If the gene is known, even from a partial sequence, then subsequent data provide for a determination of that gene - the data added to what was known (the existence of that gene) and give info on mRNA start or translation end or splice junctions, etc.

ADD COMMENT

Login before adding your answer.

Traffic: 1694 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6