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684 results • Page
2 of 14
Sort: Votes
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Views
Votes
Replies
4
votes
7
replies
620
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 25 days ago by
dsull
★ 6.0k • written 26 days ago by
VITALA
• 0
4
votes
3
replies
3.0k
views
How to solve the error 'ERROR::MATE_NOT_FOUND:Found xxx unpaired mates' when run SamToFastq
samtofasq
picard
validatesamfile
updated 11 days ago by
Pierre Lindenbaum
161k • written 11 days ago by
Lila M
★ 1.2k
4
votes
9
replies
2.2k
views
6 follow
Same sequencing sample in multiple lanes. How to analyse it?
sequencing
bowtie2
alignment
samtools
ngs
updated 16 days ago by
ST
• 0 • written 2.2 years ago by
Federico
• 0
4
votes
6
replies
3.9k
views
Taking the difference of two VCFs (or removing singletons)
genome
sequencing
singleton
SNP
filter
updated 20 days ago by
Andres
▴ 20 • written 9.7 years ago by
hermathena
▴ 40
4
votes
5
replies
467
views
Figures are disappeared from html report of SnpEff
HTML
SnpEff
21 days ago by
analyst
▴ 50
4
votes
9
replies
2.1k
views
Legend and hap files for imputation with 38 build
reference
38build
impute
imputation
20 days ago by
anna
▴ 20
4
votes
6
replies
3.8k
views
How to interpret DEXseq results in therms of significance
DEXseq
RNA-Seq
exon
updated 14 days ago by
Sara
▴ 30 • written 6.7 years ago by
Lila M
★ 1.2k
4
votes
2
replies
289
views
GRCh38.gmap file
gnomix
gmap
15 days ago by
lorena9132
▴ 10
4
votes
1
reply
271
views
LD-prune variants while maintaining a list of SNPs
snp
vcf
plink
filtering
independent
updated 14 days ago by
zx8754
11k • written 15 days ago by
Jautis
▴ 560
4
votes
1
reply
213
views
Extracting only 4-fold degenerate sites from gene sequences/alignments?
alignments
6 days ago by
J.
▴ 40
4
votes
2
replies
197
views
Duplicated sequence samtools
bowtie2
samtools
updated 6 days ago by
GenoMax
142k • written 6 days ago by
Moinuddin
• 0
4
votes
4
replies
3.0k
views
CNVkit for somatic copy number detection
cnv
cnvkit
exome
WES
updated 6 days ago by
Anitha
• 0 • written 5.1 years ago by
stephaniem
• 0
4
votes
5
replies
3.4k
views
How to compare compare two Sam files to check mapping
alignment
updated 11 days ago by
DavidStreid
▴ 90 • written 6.4 years ago by
madhu.9124
▴ 60
4
votes
8
replies
775
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
updated 23 days ago by
Alex Reynolds
35k • written 24 days ago by
J
▴ 10
4
votes
6
replies
817
views
7 follow
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 23 days ago by
dsull
★ 6.0k • written 27 days ago by
qudrat.nii
▴ 10
4
votes
6
replies
1.2k
views
Soft-clipping read ends based on read group
ancient
molecule
at
bam
DNA
RG
ends
clipping
damage
updated 12 days ago by
chenl
▴ 10 • written 2.5 years ago by
Martyna
• 0
4
votes
7
replies
437
views
How does gene length effect the number of reads mapped
RNA-seq
CPM
sequencing
6 days ago by
Chen
• 0
4
votes
15
replies
1.7k
views
how to test for differential expression in samples where a global increase in gene expression is expected
edgeR
DESeq
Differential-Expression
RNA-Seq
updated 16 days ago by
Gordon Smyth
★ 7.2k • written 5 months ago by
raplayer
▴ 10
4
votes
7
replies
533
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
updated 3 days ago by
marco.barr
▴ 130 • written 4 days ago by
diqixiaoyaoer
▴ 20
4
votes
7
replies
640
views
Very low RNA splicing rate for pulmonary AT2 cells
RNA-velocity
scVelo
scRNA-seq
splicing
20 days ago by
e.r.zakiev
▴ 210
4
votes
5
replies
4.4k
views
Tool:
GRIDSS: the Genomic Rearrangement IDentification Software Suite
Assembly
Structural-Variation
Variant-Calling
updated 3 days ago by
dario.garvan
▴ 520 • written 7.2 years ago by
d-cameron
★ 2.9k
4
votes
12
replies
4.9k
views
10 follow
How to analyze Infinium Mouse Methylation BeadChip array data?
Methylation
Experience
Illumina
Mouse
updated 11 days ago by
Tawny
▴ 180 • written 3.0 years ago by
julia_geh
▴ 20
4
votes
9
replies
491
views
Faster Needleman-Wunsch rapid global alignment of two sequences?
Needleman-Wunsch
alignment
3 days ago by
Gabriel R.
★ 2.9k
4
votes
1
reply
360
views
How to use limma to find differentially expressed genes in response to a continuous variable
limma
voom
R
20 days ago by
pairedttest
▴ 10
3
votes
3
replies
369
views
Kraken2 database
kraken
microbialdb
database
krakendb
kraken2
updated 6 days ago by
Mathew
▴ 140 • written 9 days ago by
Christopher
• 0
3
votes
4
replies
340
views
Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer version?
RNA-seq
patch
freeze
genome
updated 2 days ago by
Jeremy Leipzig
22k • written 4 days ago by
Ali
• 0
3
votes
4
replies
4.0k
views
FarmCPU - how to explain the reported 'effect'?
gwas
updated 12 days ago by
chloek88
• 0 • written 5.6 years ago by
Philipp Bayer
8.5k
3
votes
8
replies
732
views
Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA2 - where to go from here?
nf-core
16S
amplicon
dada2
ampliseq
updated 13 days ago by
Chris Dean
▴ 410 • written 17 days ago by
sovrappensiero
▴ 100
3
votes
3
replies
298
views
Gene Specific coverage from WGS data
WGS
SARS-CoV2
updated 6 days ago by
Ram
43k • written 20 days ago by
Adyasha
• 0
3
votes
7
replies
424
views
FastQC Quality per tile and per sequence behaving strange after using Cutadapt
fastqc
cutadapt
illumina
tile
paired-end
20 days ago by
salias
• 0
3
votes
2
replies
431
views
Extract protein sequence
fasta
alignment
blast
19 days ago by
anna
▴ 20
3
votes
6
replies
2.0k
views
7 follow
DNA methylation preprocessing
SWAN
DNA
methylation
Funnorm
updated 23 days ago by
Ram
43k • written 2.4 years ago by
SYOSY
▴ 10
3
votes
4
replies
763
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
27 days ago by
rahu
• 0
3
votes
4
replies
338
views
Truncated metadata file report from ENA Portal API
ena
python
9 days ago by
Giulia
• 0
3
votes
4
replies
425
views
Why gatk VariantAnnotator required bam and coverage files
gatk
VariantAnnotator
24 days ago by
QX
• 0
3
votes
5
replies
648
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
28 days ago by
n_navy
• 0
3
votes
0
replies
1.1k
views
Tool:
Sars-Cov-2 Genome & ACE 2 Exploration
sequence
alignment
SNP
genome
15 days ago by
Ibrahim Tanyalcin
★ 1.2k
3
votes
2
replies
204
views
Publish a Letter in higher impact vs Research article in lower impact
Article
Letter
Journal
updated 7 days ago by
ATpoint
82k • written 7 days ago by
jennyp0706
• 0
3
votes
3
replies
300
views
Getting the nucleotide sequence of proteins, having their UniProt IDs
Nucleotide
Genomic
Sequence
Uniprot
Proteins
updated 28 days ago by
GenoMax
142k • written 28 days ago by
Mariana
▴ 40
3
votes
3
replies
454
views
absolute path for symbolic links in Snakefile
Snakemake
updated 10 days ago by
Jesse
▴ 770 • written 4 weeks ago by
yifangt86
▴ 60
3
votes
8
replies
6.6k
views
7 follow
GATK genomicsDBimport intervals for WGS
gatk
genomicsdbimport
updated 17 days ago by
Sd
• 0 • written 5.1 years ago by
Nicolas Rosewick
11k
3
votes
9
replies
641
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 26 days ago by
Istvan Albert
100k • written 27 days ago by
Κοσμάς
• 0
3
votes
3
replies
374
views
genome assembly records not present in assembly_summary.txt
ncbi
bacteria
assembly
updated 24 days ago by
GenoMax
142k • written 24 days ago by
sapuizait
▴ 10
3
votes
5
replies
418
views
Converting CRAM to FastQ
GATK
samtools
cram
SamToFastq
fastq
16 days ago by
Maverick
▴ 10
3
votes
3
replies
289
views
what is another word for a BLAST "hit"?
blast
alignment
9 days ago by
dec986
▴ 380
3
votes
1
reply
332
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
updated 5 days ago by
dariober
14k • written 6 days ago by
Holly
▴ 30
3
votes
3
replies
375
views
Sequence read length shorter than flow cell specification
illumina
NGS
sequencing
updated 24 days ago by
swbarnes2
14k • written 24 days ago by
M
• 0
3
votes
3
replies
2.5k
views
How to make a TSS enrichment plot in ATAC-seq analysis?
ATAC
updated 25 days ago by
sp
• 0 • written 22 months ago by
Dan
▴ 180
3
votes
2
replies
385
views
what is the purpose of indexing the reference genome (Kallisto)
indexing
Kallisto
updated 24 days ago by
dsull
★ 6.0k • written 24 days ago by
Aaliya
▴ 10
3
votes
5
replies
362
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 28 days ago by
GenoMax
142k • written 28 days ago by
ahmad.sajad4541
• 0
684 results • Page
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Merging/Concatenating Vcf Files
Answer: Ti Tv ratio and their usefulness in exome sequencing
Answer: Ti Tv ratio and their usefulness in exome sequencing
Answer: How do you generate TMM normalized counts using EdgeR?
Answer: How do you generate TMM normalized counts using EdgeR?
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Comment: Super ehancers
by
Oburah
• 0
python2.7 ROSE_main.py -g HG38 -i /home/hesborn/Desktop/Data/S3vS2-2.gff -r /home/hesborn/Downloads/possorted_bam.bam.bai -o Hezz -s 12…
Answer: How to generate an Upset plot in R to plot the shared variants between cell free
by
zx8754
11k
Try this example: library(UpSetR) #example input, 4 samples, 250 variants set.seed(1); d <- data.frame(matrix(sample…
Answer: Illumina methylation EPIC V2 array
by
fu_entomology
▴ 40
We have been using SeSAMe, it works well for the most part. They have an enrichment tool called KnowYourCG. You can check it out their vign…
Answer: How to find identical sequences in genome fasta file (by Python or any possible
by
GenoMax
142k
> My final purpose are find and remove any identical sequences present in my genome fasta file. You can use `clumpify.sh` from BBMap suite…
Comment: GO analysis: Indicate genes corresponding to the pathway
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▴ 130
I think you have set a filter to get these pahways upstream from the DEG results. You should extract the IDs or names of the genes that you…
Comment: GATK Structural Variants Pipeline - Steps
by
Bioinformatics_begginner
▴ 20
Thank you for the response, I mean those tools aren't all the steps from fasta to vcf. Would be key understanding the aligner, reference us…
Comment: seqtk subseq in.fastq list.txt > out.fastq not extracting full sequence from
by
GenoMax
142k
Please accept the answer (green check mark) to provide closure to this thread.
Answer: GATK Structural Variants Pipeline - Steps
by
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161k
> A structural variation pipeline for short-read sequencing https://github.com/broadinstitute/gatk-sv
Comment: GATK Structural Variants Pipeline - Steps
by
DGTool
▴ 20
If it is just the tools that they used which you are looking for, the first link in the overview section of that page leads to the followin…
Comment: GO analysis: p-value range
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4.6k
It is just a warning that you add a new scale to a ggplot2 plot, that already has an existing one, which will get replaced. Nothing to worr…
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▴ 130
If you write me the code you used I can help you more
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When I run the code, the following message appears: Scale for y is already present. Adding another scale for y, which will replace…
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• 0
In the case of other samples, the total number of genes is at least 3,000 more than that one, but it seems to be a relatively small number.…
Comment: How to hide label in layout file of MCscan (python version)
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jinyi
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Command line is as follows: python -m jcvi.graphics.synteny Cs.Cm.i1.blocks Cs_Cm.bed layout2.txt
Comment: Add stats to the plot
by
marco.barr
▴ 130
Thank you, it seems to be clearer. Your Condition already has two factorial levels. You can replace these names in groups 1 and 2. da…
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