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1,000 results • Page 1 of 20
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5
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3
replies
239
views
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 3 days ago by 141k • written 3 days ago by • 0
0
votes
0
replies
162
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
4 days ago by yxwucq • 0
4
votes
2
replies
283
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 10 days ago by 43k • written 10 days ago by ▴ 10
7
votes
7
replies
1.2k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 11 days ago by ★ 2.5k • written 12 days ago by • 0
1
vote
2
replies
339
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
9 days ago by Javier • 0
16
votes
10
replies
1.0k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 28 days ago by 5.3k • written 29 days ago by ★ 2.4k
1
vote
1
reply
219
views
Forum: Can I use this community for software development?
software-development
updated 4 weeks ago by 43k • written 4 weeks ago by • 0
5
votes
2
replies
384
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 4 weeks ago by 54k • written 4 weeks ago by • 0
0
votes
0
replies
172
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 5 weeks ago by 43k • written 5 weeks ago by • 0
1
vote
3
replies
475
views
Forum: Development plan for a bioinformatics database developer/coder
developer
6 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
673
views
Forum: Bioinformatics career transition
career forum
updated 6 weeks ago by ★ 16k • written 6 weeks ago by ▴ 20
4
votes
2
replies
474
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
7 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
764
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
7 weeks ago by jared.andrews07 ★ 16k
0
votes
0
replies
161
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 7 weeks ago by 43k • written 7 weeks ago by ★ 1.2k
0
votes
2
replies
369
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
7 weeks ago by Dave Carlson ★ 1.7k
31
votes
12
replies
950
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
8 weeks ago by karlaarz ▴ 90
5
votes
4
replies
554
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 9 weeks ago by 141k • written 9 weeks ago by ▴ 10
1
vote
2
replies
368
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 9 weeks ago by 82k • written 9 weeks ago by ▴ 20
8
votes
7
replies
790
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 9 weeks ago by ▴ 120 • written 12 weeks ago by • 0
1
vote
0
replies
277
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 9 weeks ago by 161k • written 9 weeks ago by ★ 1.4k
4
votes
2
replies
372
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 11 weeks ago by ★ 5.8k • written 11 weeks ago by ▴ 10
8
votes
6
replies
2.8k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by 10k
0
votes
0
replies
200
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 12 weeks ago by 43k • written 12 weeks ago by ▴ 20
2
votes
3
replies
434
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
3 months ago by Ajay A Shettigar • 0
17
votes
11
replies
981
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.2k
views
Forum: How to learn bioinformatics?
learning
updated 3 months ago by ★ 1.3k • written 23 months ago by ▴ 20
26
votes
27
replies
2.0k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 3 months ago by ★ 5.8k • written 3 months ago by ▴ 40
7
votes
6
replies
730
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 3 months ago by 43k • written 3 months ago by ▴ 20
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
2
votes
1
reply
420
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
3 months ago by LauferVA 4.2k
49
votes
20
replies
2.0k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 3 months ago by ★ 27k
4
votes
3
replies
759
views
Forum: How to get started in bioinformatics
students
updated 3 months ago by 43k • written 3 months ago by ▴ 10
2
votes
2
replies
581
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 3 months ago by 43k • written 4 months ago by ▴ 40
0
votes
2
replies
3.2k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by 141k • written 4 months ago by ▴ 10
5
votes
3
replies
605
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 4 months ago by ★ 27k • written 4 months ago by ▴ 20
6
votes
2
replies
660
views
Forum: how to start Bioinformatics?
basics
updated 4 months ago by ★ 27k • written 4 months ago by • 0
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
926
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 4 months ago by 43k • written 4 months ago by • 0
1
vote
1
reply
486
views
Forum: want to work in open source project/project in bioinformatics
project
updated 4 months ago by 43k • written 5 months ago by • 0
3
votes
5
replies
1.0k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 4 months ago by 43k • written 5 months ago by • 0
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by Istvan Albert 100k
3
votes
2
replies
621
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 5 months ago by ▴ 10 • written 5 months ago by ▴ 40
0
votes
0
replies
369
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 5 months ago by 43k • written 5 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.4k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
451
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
6 months ago by ishwarvh • 0
0
votes
0
replies
354
views
Forum: Automated Checks And QA
retrospective
6 months ago by Sasha ▴ 840
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 6 months ago by ▴ 10
21
votes
7
replies
11k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 6 months ago by ▴ 100 • written 8.1 years ago by 11k
1,000 results • Page 1 of 20
Recent Votes
A: Blast Settings For Short Sequences
A: Blast Settings For Short Sequences
Comment: Heatmap and rna-seq
Answer: Heatmap and rna-seq
Comment: Heatmap and rna-seq
A: should FASTA files be sorted before indexed with SAMtools?
Answer: A faidx-indexed FASTA format file or a FASTA format file
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Scholar to Alex Reynolds 35k
Popular Question to chaco001 ▴ 40
Commentator to GenoMax 141k
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Recent Replies
Answer: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC: by Mensur Dlakic ★ 27k
They say that we remember better things we figured out on our own, rather than things we were taught. You were literally two clicks away fr…
Comment: Heatmap and rna-seq by dsull ★ 5.8k
https://maayanlab.cloud/clustergrammer/ It's a web-based tool (can also be a python jupyter widget) that I find useful for exploring a pat…
Answer: Limma Analysis Agilent Microarray Data (GPL1708) by Gordon Smyth ★ 7.0k
There is an Agilent array case study in the limma User's Guide, which you might find helpful and somewhat simpler. I don't recommend col…
Answer: Correct way to compare multiple treaments between RNA-Seq samples using edgeR? by Gordon Smyth ★ 7.0k
The comparison you've done seems exactly right to test whether treatment 2 modifies or enhances the effect of treatment 1. Have you exam…
Answer: How to use limma to find differentially expressed genes in response to a continu by Gordon Smyth ★ 7.0k
There's no need for form a contrast because the score variable is already in the model. A continuous variable is its own contrast. Just run…
Comment: Create a new bed file with all pairwise combinations between two other bed files by J ▴ 10
Oh wow, bedops closest-features, how did I overlook that!! I didn't know about it; thank you! Your awk script is beautiful. This worked per…
Comment: Create a new bed file with all pairwise combinations between two other bed files by J ▴ 10
Thank you Pierre! I'll try this out with my full data set.
Answer: DNA methylation preprocessing by cao510927 ▴ 30
I happend to see one paper doing the similar thing, share it with you guys, "A systematic evaluation of normalization methods and probe rep…
Answer: Create a new bed file with all pairwise combinations between two other bed files by Alex Reynolds 35k
I think Pierre's answer might be correct for your test input, but it doesn't account for the start position of the reference element and so…
Comment: Highest variable features in single cell data by bk11 ★ 2.4k
You need to be clear about how `Seurat` defines highly variable genes here. Highly variable genes are the genes that have very high express…
Comment: Heatmap and rna-seq by GenoMax 141k
Please include a link when you are recommending a tool. There can be programs with similar names.
Answer: Heatmap and rna-seq by dsull ★ 5.8k
Check out clustergrammer from Avi Ma'ayan's lab.
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by Ram 43k
Simple: ```r data <- lapply(files, read_tsv) data <- Reduce(merge, data) # or Reduce(full_join, data) ``` <a href="" title="Text added bec…
Answer: Source other conda environments in a nextflow pipeline when nextflow itself is i by ATpoint 82k
You can make each process use a dedicated environment, see https://www.nextflow.io/docs/latest/conda.html#use-existing-conda-environments …
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by BioinfGuru ★ 1.7k
Hi, Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
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