Entering edit mode
3.4 years ago
Nic
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0
Hi,
I am currently finishing my Master's degree and I am relatively new to this subject. I would like to ask if anyone knows how to do demultiplexing via linux. Specifically I have found this example (https://astrobiomike.github.io/amplicon/demultiplexing) which I was able to implement by downloading all the programs I need to work with, however I can't work with my own files and sequences. I would greatly appreciate it if someone could help me.
Thank you,
Nikos
Are you getting errors?
Yes, I think I'm making mistakes, so I'm getting a lot of erros. I don't know how to insert my file into the above example to make it work.
You will need to make a mapping file as shown in the example. That appears to be a tab limited file. Make a note of the column headers and make sure you format your file right.
Please post example data, the function/command you are using and errors you are getting. That would help forum members to address the errors.