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897 results • Page
1 of 18
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Rank
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1
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2
replies
21
views
Genotyping sites with N in reference genome
GATK
UnifiedGenotyper
3 minutes ago by
shpak.max
▴ 50
0
votes
1
reply
25
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 23 minutes ago by
yura.grabovska
▴ 70 • written 51 minutes ago by
ev97
▴ 20
0
votes
1
reply
16
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
meta
how-to
ncbi
forum
updated 2 minutes ago by
Pierre Lindenbaum
161k • written 51 minutes ago by
hophuquy0944
• 0
0
votes
0
replies
17
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
1 hour ago by
diqixiaoyaoer
▴ 10
0
votes
2
replies
38
views
Sequencing Depth (Read Depth) Calculations
depth
updated 1 hour ago by
GenoMax
142k • written 1 hour ago by
jiahangkui1234
• 0
0
votes
1
reply
26
views
Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
annotation
vcf
vep
zygosity
deepvariant
1 hour ago by
asalimih
▴ 60
0
votes
0
replies
22
views
Is it okay to assemble my 12 metatranscriptome samples with Trinity separately?
trinity
metatranscriptome
1 hour ago by
jway
• 0
1
vote
2
replies
60
views
Cannot install bwa-mem2 via conda
conda
mamba
python
code
updated 1 hour ago by
Istvan Albert
100k • written 2 hours ago by
jsmith120f
• 0
2
votes
2
replies
116
views
genome finishing
finishing
genome
updated 2 hours ago by
nd48
▴ 20 • written 6 hours ago by
trezini
• 0
1
vote
5
replies
121
views
Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
circos
atacseq
updated 2 hours ago by
Pierre Lindenbaum
161k • written 3 hours ago by
biology_inform
▴ 50
0
votes
3
replies
108
views
Add stats to boxplot in R
stats
R
updated 1 hour ago by
Lélé
• 0 • written 4 hours ago by
Ghada
• 0
0
votes
0
replies
46
views
Seurat v5 and how to correctly integrate across multiple experiments
Seurat
scRNA
R
updated 2 hours ago by
ATpoint
82k • written 3 hours ago by
yura.grabovska
▴ 70
0
votes
0
replies
38
views
Difference in number of reads when using duplex and simplex base calling with Dorado
base
calling
nanopore
simplex
dorado
duplex
3 hours ago by
Lélé
• 0
0
votes
3
replies
87
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
Degrees
python
Degree
offtopic
updated 3 hours ago by
Joe
21k • written 3 hours ago by
kuttibiotech2009
▴ 30
0
votes
2
replies
99
views
calculating genomic coverage/ base overlap in R
bioinoformatics
genomics
updated 3 hours ago by
GenoMax
142k • written 4 hours ago by
Sayantani
• 0
0
votes
2
replies
70
views
Annotating file using bcftools
annotation
plink
bcftools
updated 4 hours ago by
Pierre Lindenbaum
161k • written 6 hours ago by
kl
▴ 10
0
votes
8
replies
205
views
Duplicated reads (IDs) from nanopore sequencing
bam
nanopore
sequencing
updated 1 hour ago by
GenoMax
142k • written 10 hours ago by
njornet
▴ 20
0
votes
3
replies
96
views
Problem with Calling Variants from RNA-Seq data
vcf
gatk
benchmark
giab
rna-seq
6 hours ago by
Esraa
• 0
0
votes
1
reply
96
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
updated 5 hours ago by
ATpoint
82k • written 6 hours ago by
egascon
• 0
1
vote
1
reply
90
views
Getting differential ChIP-seq peaks between conditions after calling consensus peaks
MAnorm
ChIP-seq
consensus
peaks
updated 7 hours ago by
ATpoint
82k • written 8 hours ago by
Alexandra
• 0
1
vote
1
reply
175
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
updated 8 hours ago by
dariober
14k • written 1 day ago by
Holly
▴ 10
0
votes
1
reply
100
views
Filtering VCF files based on VAF giving incorrect results
bcftools
GATK
Bioinformatics
picard
VCF
updated 9 hours ago by
Pierre Lindenbaum
161k • written 12 hours ago by
Arton
• 0
0
votes
0
replies
50
views
ReactomeGSA
ReactomeGSA
9 hours ago by
Shaimaa Gamal
▴ 10
0
votes
0
replies
47
views
News:
New course: Manipulation of NGS Data for Genomic and Population Genetics Analyses
KeepLearning
TScourses
9 hours ago by
Transmitting Science communication
▴ 70
0
votes
1
reply
88
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 5 hours ago by
GenoMax
142k • written 10 hours ago by
chrisk
• 0
0
votes
1
reply
92
views
perseus software (version 1.5.5)
preseus
mass
spectrometry
data
updated 3 hours ago by
Joe
21k • written 13 hours ago by
iqra
• 0
0
votes
0
replies
65
views
Why the shape of RCS is contradictory to the p value for nonlinearity.
RCS
ggplot2
ggplot
R
updated 14 hours ago by
GenoMax
142k • written 15 hours ago by
Dude
• 0
0
votes
0
replies
61
views
NEURON, Brian2, NEST, or PyNN
NEST
NEURON
PyNN
Brian2
15 hours ago by
brunofelicianodeomena
• 0
1
vote
2
replies
153
views
Alphafold 3 pLDDT coloring scheme in chimerax?
ChimeraX
Alphaphold
Alphaphold3
updated 10 hours ago by
colindaven
6.4k • written 17 hours ago by
mbrav005
• 0
0
votes
1
reply
111
views
Polish a large genome with Pilon
Pilon
Pacbio
Polish
Illumina
13 hours ago by
sansan_96
▴ 90
0
votes
1
reply
112
views
Doubt about the process of annotation, detection, identification and classification of transposable elements
annotation
TransposableElements
updated 19 hours ago by
Mensur Dlakic
★ 27k • written 20 hours ago by
MarcosCosta
• 0
2
votes
2
replies
170
views
Genome Visualization Tools
bacterial
genome
updated 21 hours ago by
GenoMax
142k • written 21 hours ago by
dlera.lozano
▴ 10
2
votes
3
replies
177
views
RSeQC : infer_experiment.py Error: Could not retrieve index file
RSeQC
RNA-Seq
updated 20 hours ago by
Ram
43k • written 21 hours ago by
Prawesh
• 0
0
votes
3
replies
212
views
Using ggplotly in R
ggplot
ggplotly
updated 1 hour ago by
jared.andrews07
★ 16k • written 22 hours ago by
jen
▴ 10
0
votes
1
reply
137
views
Why does assigning genes with biomart give me different values than using a transcripts_to_genes.txt file?
biomart
RNAseq
kallisto
updated 22 hours ago by
GenoMax
142k • written 22 hours ago by
bioinfo
▴ 150
0
votes
3
replies
223
views
RNAseq RNA content
mRNA
Linux
rRNA
RNA-seq
RNA
updated 22 hours ago by
noodle
▴ 580 • written 1 day ago by
doramora
▴ 10
0
votes
1
reply
123
views
Post-imputation QC for input into GWAS analyses
gwas
prs
updated 23 hours ago by
LauferVA
4.2k • written 1 day ago by
graeme.thorn
▴ 100
0
votes
2
replies
153
views
Using Cutadapt to trim adapters from paired-end small RNA sequence data
trimming
adapters
cutadapt
sRNA-seq
smallRNA
9 hours ago by
melissa.joubert
• 0
0
votes
0
replies
92
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
1 day ago by
ramiro.barrantes
• 0
4
votes
1
reply
180
views
Extracting only 4-fold degenerate sites from gene sequences/alignments?
alignments
1 day ago by
J.
▴ 40
0
votes
0
replies
92
views
Is there a real ground truth for CNV data?
CNV
1 day ago by
jennyp0706
• 0
0
votes
3
replies
189
views
Biomart issue, why so few 3'utrs?
utr
biomart
updated 1 day ago by
GenoMax
142k • written 1 day ago by
RNAseqer
▴ 270
1
vote
1
reply
127
views
sci-RNA-seq
Seurat
sci-RNA-seq
updated 23 hours ago by
Ram
43k • written 1 day ago by
kilcdincer
▴ 10
0
votes
0
replies
82
views
Retrieving ceRNA data from the starBase or ENCORI Web API
ceRNA
ENCORI
WebAPI
microRNA
starBase
updated 1 day ago by
GenoMax
142k • written 1 day ago by
Bhavya
• 0
4
votes
4
replies
2.9k
views
CNVkit for somatic copy number detection
cnv
cnvkit
exome
WES
updated 1 day ago by
Anitha
• 0 • written 5.1 years ago by
stephaniem
• 0
1
vote
7
replies
357
views
Overlapping Ranges within Granges object
Genomicranges
IRanges
GRanges
13 hours ago by
ntsopoul
▴ 60
0
votes
3
replies
186
views
How to access GWAVA software of data
GWAVA
updated 1 day ago by
GenoMax
142k • written 1 day ago by
nonaddldy
▴ 10
0
votes
1
reply
167
views
Inquiry about deseq2 transformation
RNA-seq
deseq2
updated 1 day ago by
ATpoint
82k • written 1 day ago by
Chen
• 0
1
vote
1
reply
164
views
The total expressed genes in RNA-Seq data
RNA-SEQ
updated 1 day ago by
ATpoint
82k • written 1 day ago by
Pegasus
▴ 100
0
votes
1
reply
125
views
What is the bin size for Bamcompare?
chip-seq
deeptools
bamcompare
updated 20 hours ago by
Ram
43k • written 1 day ago by
Emily
▴ 20
897 results • Page
1 of 18
Recent Votes
Answer: Genotyping sites with N in reference genome
A: how to combine RNA seq data from 4 lanes
Answer: Cannot install bwa-mem2 via conda
Answer: Sub-sampling a BAM to a fixed number of reads
Comment: Downsample BAM file to specific amount of reads
Answer: Long reads and fixing of mate-pair issues/marking duplicates with samtools
Answer: Long reads and fixing of mate-pair issues/marking duplicates with samtools
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Recent Replies
Answer: Genotyping sites with N in reference genome
by
Pierre Lindenbaum
161k
> Does UnifiedGenotyper skip/ignore sites where the reference genotype is N yes. The variant is in YOUR data, not in the reference where '…
Comment: Can 5' and 3' scRNAseq be processed with the same pipeline?
by
yura.grabovska
▴ 70
Do you have samples that should in theory have a good degree over overlap in terms of cell types across the two sequencing types? I would …
Comment: Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
by
asalimih
▴ 60
I found out that vep is only checking the GT field therefore assigning HOM to the Zygosity. Therefore the problem should be from deepvarian…
Comment: Add stats to boxplot in R
by
Lélé
• 0
To remove "Kruskall Wallis" you can try adding the argument label="p" or label = "p.signif" which displays the p-value or *** like this: …
Answer: Sequencing Depth (Read Depth) Calculations
by
GenoMax
142k
You could also use `pandepth` ([**LINK**][1]) or `mosdepth` ([**LINK**][2]) as well. These will allow you to be flexible about windowed cov…
Comment: Sequencing Depth (Read Depth) Calculations
by
Pierre Lindenbaum
161k
it looks looks ok to me. <!-- This cmd works and gives me 40.5235, I was wondering whether this cmd is right or wrong, and does anyone hav…
Comment: Add stats to boxplot in R
by
Ghada
• 0
Great. thats work. Do you know how can I remove the kruskall-Wallis name from the plot? Also I would like to draw a line under the kruskall…
Comment: Using ggplotly in R
by
jared.andrews07
★ 16k
Again, this is not particularly difficult to do with [plotly itself](https://plotly.com/r/line-and-scatter/#adding-color-and-size-mapping) …
Comment: Duplicated reads (IDs) from nanopore sequencing
by
GenoMax
142k
If you only have supplementary alignments then they may be caused by reasons mentioned here --> https://www.biostars.org/p/308853/ I did no…
Comment: Cannot install bwa-mem2 via conda
by
Pierre Lindenbaum
161k
> Please do not paste screenshots of plain text content, it is counterproductive. You can copy paste the content directly here (using the c…
Answer: Cannot install bwa-mem2 via conda
by
Istvan Albert
100k
The problem is caused by running the ARM-based conda. Bioinformatics software is compiled for Intel based CPUs only. Uninstall that versio…
Comment: Duplicated reads (IDs) from nanopore sequencing
by
marco.barr
▴ 110
[https://www.biostars.org/p/9559284/][1] you could try this [1]: https://www.biostars.org/p/9559284/
Comment: Duplicated reads (IDs) from nanopore sequencing
by
marco.barr
▴ 110
it's also true that now I only have one pod5 folder, thanks anyway for clarifying the focus of the issue. Yes, they are probably secondary …
Comment: Applying the metacell2 algorithm using python
by
Wayne
★ 2.0k
I'm unsure. I will say that looking for [ normalize](https://github.com/search?q=repo%3Atanaylab%2Fmetacells%20normalize&type=code) among t…
Comment: Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
by
Pierre Lindenbaum
161k
> Please do not paste screenshots of plain text content, it is counterproductive. You can copy paste the content directly here (using the c…
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