Limit : this week
all time today this week this month this year
195 results • Page 1 of 4
Sort: replies
Rank Views Votes Replies
9
votes
23
replies
9.8k
views
Tool: BioLabDonkey - new Mac program for molecular biologists
software biolabdonkey
2 days ago by vytarasov ▴ 180
10
votes
16
replies
17k
views
7 follow
How Can I Count Snps In My Final Vcf Files
SNP
updated 6 days ago by 161k • written 5.8 years ago by ▴ 180
13
votes
14
replies
869
views
High Malat-1 expression in single cell data
single-cell
updated 5 days ago by ▴ 40 • written 14 days ago by ▴ 40
10
votes
14
replies
696
views
6 follow
Bacterial plasmid analysis
bacteria plasmid wgs hybridassembly sequencing
updated 6 days ago by 142k • written 12 days ago by • 0
9
votes
13
replies
4.8k
views
Custom Reference panel creation for data imputation from .vcf files
imputation reference panel
updated 3 days ago by ▴ 50 • written 6.4 years ago by ▴ 490
2
votes
13
replies
2.9k
views
6 follow
CNVKIT - unable to produce scatter and diagram pdfs
cnvkit
updated 1 day ago by • 0 • written 4.8 years ago by ▴ 60
4
votes
12
replies
4.8k
views
10 follow
How to analyze Infinium Mouse Methylation BeadChip array data?
Methylation Experience Illumina Mouse
updated 4 days ago by ▴ 180 • written 3.0 years ago by ▴ 20
0
votes
11
replies
479
views
In IGV is this a heterogeneous mutation or false call?
mutations IGV heterogeneous
1 day ago by Tuck898 • 0
0
votes
9
replies
1.5k
views
kinannote software installation problems
kinannote
updated 5 days ago by • 0 • written 5.7 years ago by ▴ 30
1
vote
9
replies
358
views
UMI-Tools knee-method has great influence on the results of white list
single-cell whitelist UMI RNA UMI-Tools
updated 3 days ago by 19k • written 6 days ago by ★ 1.8k
0
votes
8
replies
417
views
Different output for read length
samtools BAM
4 days ago by marco.barr ▴ 100
2
votes
8
replies
319
views
joint callset and vcf sorting, unknown TAG issue
sort bcftools GLNexus merge VCF
updated 1 day ago by 161k • written 1 day ago by ▴ 100
9
votes
7
replies
7.1k
views
Align paired and unpaired reads simultaneously using Bowtie2?
alignment
updated 2 days ago by • 0 • written 5.7 years ago by ▴ 20
1
vote
7
replies
503
views
Question regarding WGCNA
WGCNA Network-construction
4 days ago by deepak • 0
0
votes
7
replies
397
views
RNAseq one control two conditions, shared and exclusive genes
conditions RNAseq multiple Rstudio
updated 22 hours ago by ▴ 20 • written 14 days ago by • 0
1
vote
7
replies
586
views
Filtering Multi-sample VCF file for all except one Genotype
variant SNP VCF
updated 3 days ago by 161k • written 6 days ago by • 0
0
votes
7
replies
493
views
Applying the metacell2 algorithm using python
python single-cell scanpy metacell2
updated 5 days ago by ★ 2.0k • written 7 days ago by ▴ 140
4
votes
7
replies
364
views
How does gene length effect the number of reads mapped
RNA-seq CPM sequencing
6 hours ago by Chen • 0
6
votes
7
replies
335
views
Longest transcript variant per gene
transcript longest variant orthofinder
1 day ago by sansan_96 ▴ 90
0
votes
6
replies
377
views
Is it Possible to "Merge" data from Replicates within a VCF SNP File
snp vcf genomics plink
updated 8 hours ago by 43k • written 3 days ago by • 0
0
votes
6
replies
2.1k
views
Copy number Variation analysis using conumee
R software error sequencing genome
updated 4 days ago by 142k • written 3.2 years ago by ▴ 10
4
votes
6
replies
1.2k
views
Soft-clipping read ends based on read group
ancient molecule at bam DNA RG ends clipping damage
updated 5 days ago by ▴ 10 • written 2.5 years ago by • 0
4
votes
6
replies
396
views
RNA seq analysis
DESeq RNA-seq
updated 17 hours ago by 4.6k • written 5 days ago by ▴ 10
3
votes
6
replies
465
views
install package: package ‘gmwm’ is not available for this version of R
r
updated 1 day ago by ▴ 20 • written 8 days ago by • 0
1
vote
5
replies
346
views
Comparative Analysis of Algorithms for Implementing the FM-Index: Correctness, Complexity, and Use Cases
fmindex bwt
updated 5 days ago by 54k • written 6 days ago by • 0
0
votes
5
replies
304
views
A question about reference genome for creating the consensus sequence
fasta fa cram genome
5 days ago by me • 0
5
votes
5
replies
291
views
Error in dimnames(x) <- dn : length of 'dimnames' [2] not equal to array extent
EGSEA
6 days ago by Chris ▴ 280
6
votes
5
replies
2.3k
views
Tophat multiple or unique mapping criteria
RNA-Seq
updated 1 day ago by • 0 • written 6.7 years ago by • 0
1
vote
5
replies
425
views
HCL database download
HCL
updated 6 days ago by 43k • written 10 days ago by ▴ 20
2
votes
5
replies
521
views
Tissue-specific DEG analysis with DEseq2
DEseq2 RNA-seq DEG R
updated 1 day ago by ▴ 40 • written 10 days ago by ▴ 30
4
votes
5
replies
3.4k
views
How to compare compare two Sam files to check mapping
alignment
updated 5 days ago by ▴ 90 • written 6.4 years ago by ▴ 60
0
votes
5
replies
293
views
Too many unpaired forward reads found by Trimmomatic
Trimmomatic
17 hours ago by SilhouetteQ • 0
2
votes
5
replies
2.5k
views
identifying transgene insertion site in WGS
WGS insertion site trangene de-novo soap
updated 5 days ago by • 0 • written 5.1 years ago by ★ 1.8k
0
votes
5
replies
298
views
How to convert normalized BigWig file to count matrix?
count-matrix BigWig
1 day ago by feather-W • 0
0
votes
5
replies
253
views
Percentage coverage of reference genome by de novo genome
SAMtools BWA alignment ddRAD
updated 5 days ago by 21k • written 5 days ago by • 0
2
votes
5
replies
129
views
What does the 'E%' represent in BUSCO results?
BUSCO
updated 2 hours ago by 8.5k • written 6 hours ago by • 0
2
votes
5
replies
381
views
Importing a fastq file
Fastq
updated 4 days ago by ▴ 120 • written 5 days ago by • 0
2
votes
4
replies
5.6k
views
44% Successfully Assigned Fragments with featureCounts after 85% uniquely mapped reads with STAR
rna-seq alignment RNA-Seq featureCounts STAR
updated 16 hours ago by ▴ 340 • written 4.9 years ago by ▴ 70
0
votes
4
replies
400
views
Highest variable features in single cell data
single-cell
6 days ago by carolofharvest ▴ 40
1
vote
4
replies
350
views
featureCounts output summary assigned value higher than uniquely mapped reads from HISAT2
RNA-seq featureCounts HISAT
updated 10 hours ago by 142k • written 5 days ago by • 0
1
vote
4
replies
322
views
Contig assembly task, errors
dna genetics contig assembly
updated 8 hours ago by 43k • written 5 days ago by • 0
0
votes
4
replies
3.6k
views
SNPEff database building error
SNP
updated 7 hours ago by • 0 • written 7.1 years ago by • 0
0
votes
4
replies
280
views
Correlation Analysis
statistics methylation NGS expression
2 days ago by Researcher ▴ 30
0
votes
4
replies
356
views
Adata.raw.X in LIANA, something wrong with conversion from Seurat to adata in python.
k
23 hours ago by beginner123 • 0
6
votes
4
replies
13k
views
Trimmomatic: What is the difference between paired and unpaired output files in paired-end mode
trimmomatic paired-end
updated 2 days ago by • 0 • written 6.4 years ago by ▴ 130
3
votes
4
replies
298
views
Truncated metadata file report from ENA Portal API
ena python
3 days ago by Giulia • 0
1
vote
4
replies
400
views
Can I readmap short reads to rDNA references?
rDNA assembly
updated 3 days ago by 142k • written 26 days ago by • 0
3
votes
4
replies
4.0k
views
FarmCPU - how to explain the reported 'effect'?
gwas
updated 5 days ago by • 0 • written 5.5 years ago by 8.5k
1
vote
4
replies
372
views
Understanding STAR output (Aligned.out.sam file)
STAR paired-end read
6 days ago by heelpPlease • 0
1
vote
4
replies
486
views
cellranger error message
multiplexing cellranger
updated 3 days ago by • 0 • written 3 months ago by ▴ 10
195 results • Page 1 of 4
Recent Votes
Comment: Getting the coding_sequence.fasta from the .gff file from the AUGUSTUS gene pred
A: How can I convert -log10 (p-value) to p-value?
Comment: What does the 'E%' represent in BUSCO results?
Answer: What does the 'E%' represent in BUSCO results?
Answer: Combine Two Affymetrix Datasets With Same Platform
Comment: Kraken2 database
Comment: How does gene length effect the number of reads mapped
Recent Locations • All
WCIP | Glasgow | UK, 1 minute ago
Taiwan, 5 minutes ago
India, 6 minutes ago
Italy, 8 minutes ago
Adelaide, 8 minutes ago
Hong Kong, 12 minutes ago
Australia, 15 minutes ago
Recent Awards • All
Popular Question to jiyoung ▴ 20
Autobiographer to Jeol ▴ 20
Popular Question to Pegasus ▴ 100
Popular Question to RNAseqer ▴ 270
Scholar to kalavattam ▴ 190
Scholar to dthorbur ★ 2.0k
Commentator to Santosh Anand 5.7k
Recent Replies
Answer: Is there any way to modify this pie chart ? by marco.barr ▴ 100
Hi, you can add the labels outside chart using `geom_label_repel` from `ggrepel` package. Check out this guide [https://r-charts.com/part…
Answer: Snakemake wrapper issue by Wei-Chen Pan • 0
Hi, did you solve your problem ? I also encounter same issue but it's `wrapper:"v3.9.0/bio/picard/mergevcfs"`. In my log file, it said:…
Comment: What does the 'E%' represent in BUSCO results? by Philipp Bayer 8.5k
No worries! I found the commit with the maths too: https://gitlab.com/ezlab/busco/-/blame/master/src/busco/busco_tools/hmmer.py?page=2#L135…
Comment: What does the 'E%' represent in BUSCO results? by 林明德 • 0
Thank you for your answer! It really helped me resolve a doubt!
Answer: What does the 'E%' represent in BUSCO results? by Philipp Bayer 8.5k
Ah your edit solved this. 50 BUSCOs contain internal stop codons, indicating they might be erroneous gene models, or the genes themselves a…
Answer: MGLTools does not work in windows 11 by Ashfaq • 0
In my case, I did not see iGPU. There is only one option, Intel UHD Graphics. What I need to do?
Comment: What does the 'E%' represent in BUSCO results? by Philipp Bayer 8.5k
funny enough i tried the same thing (i've used BUSCO heaps times but never saw the E:! even the manual lists 'C:89.0%[S:85.8%,D:3.2%],F:6.9…
Comment: What does the 'E%' represent in BUSCO results? by GenoMax 142k
According to ChatGPT: In BUSCO, the "E" category represents "End" or "Endof" gene fragments. These are orthologous groups for which the ge…
Comment: Help with IGV abbreviation by GeneC • 0
Thank you for the link and details
Comment: Help with IGV abbreviation by GeneC • 0
Thank you very much for helping with the details.
Comment: How does gene length effect the number of reads mapped by Chen • 0
thank you :)
Answer: Filtering based on alternate allelic balance by Arton • 0
I found the answer when using bcftools. Is there is a way to do this with FilterVcf? bcftools filter --include '(FMT/AD[0:1])/(FMT/A…
Comment: Help with IGV abbreviation by GenoMax 142k
Those are SAM format fields. Check section 1.4 here: https://samtools.github.io/hts-specs/SAMv1.pdf > 1:2114:12111:13792 That is part of …
Answer: Help with IGV abbreviation by Mathew ▴ 130
Hi, here is a breakdown of each part that you asked about: **flag 99**: This indicates various properties of the read alignment. In this c…
Comment: Kraken2 database by Mathew ▴ 130
I don't see any databases with just pathogenic bacteria genomes from just a quick search, I would imagine that using the Standard-16 databa…
Traffic: 2113 users visited in the last hour
Content Search
Users
Tags
Badges
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6