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I would probably collapse overlapping regions with `reduce` to create the second GRanges. Can you add some representative data? Using dput?
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I have one Granges object and not two. Every IRanges entry is the critical region of the gRNA. Can this still work?
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FYI if you also have this problem, this seems to be a pretty good solution: https://github.com/harvardinformatics/degenotate
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AFAIK "MANE" project is only for human protein-coding genes.
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The MANE-select transcripts are pairs of identically annotated transcripts in ReqSeq and Ensembl. The 3' UTR is the region where otherwise …
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Please understand that this impossibly can be answered without any code or plots, or details in general. "Hey my car does not start, it mak…
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