0
votes
0
replies
64
views
1
vote
0
replies
64
views
15 hours ago by
Physalia-courses
★
2.6k
4
votes
9
replies
415
views
updated 19 hours ago by
Aleksandra
▴
180
•
written 1 day ago by
young_bioinformatician
▴
240
0
votes
0
replies
94
views
updated 23 hours ago by
GenoMax
154k
•
written 1 day ago by
oliverhooker
▴
110
846
votes
170
replies
215k
views
112 follow
updated 10 days ago by
Biostar
3.6k
•
written 8.9 years ago by
Istvan Albert
103k
17
votes
12
replies
826
views
6 follow
updated 1 day ago by
Istvan Albert
103k
•
written 5 days ago by
firestar
★
1.7k
2
votes
1
reply
138
views
updated 1 day ago by
ATpoint
89k
•
written 1 day ago by
carolofharvest
▴
50
0
votes
0
replies
100
views
4
votes
4
replies
236
views
updated 1 day ago by
Elisabeth Gasteiger
★
2.4k
•
written 1 day ago by
Luwell
•
0
6
votes
2
replies
175
views
updated 1 day ago by
Aleksandra
▴
180
•
written 1 day ago by
PolenP
▴
10
0
votes
2
replies
235
views
0
votes
1
reply
134
views
updated 1 day ago by
lieven.sterck
16k
•
written 1 day ago by
aj123
▴
130
0
votes
1
reply
141
views
updated 1 day ago by
dthorbur
★
3.1k
•
written 1 day ago by
yesquokkan
•
0
2
votes
6
replies
489
views
updated 1 day ago by
strayeroliver
•
0
•
written 3 days ago by
strayeroliver
▴
10
0
votes
2
replies
220
views
updated 1 day ago by
Mensur Dlakic
★
30k
•
written 2 days ago by
Ria
•
0
5
votes
12
replies
1.0k
views
6 follow
updated 2 days ago by
Zhenyu Zhang
★
1.3k
•
written 9 days ago by
vernonlim98
•
0
1
vote
4
replies
319
views
0
votes
6
replies
367
views
updated 2 days ago by
GenoMax
154k
•
written 3 days ago by
pranavdatar01
•
0
0
votes
0
replies
125
views
0
votes
2
replies
316
views
0
votes
1
reply
170
views
updated 2 days ago by
Mensur Dlakic
★
30k
•
written 2 days ago by
alevbozan18
•
0
0
votes
2
replies
1.0k
views
0
votes
0
replies
138
views
0
votes
0
replies
164
views
0
votes
2
replies
240
views
2 days ago by
mf810
•
0
2
votes
3
replies
306
views
0
votes
4
replies
1.7k
views
updated 3 days ago by
Ales
▴
70
•
written 5.5 years ago by
xiaoleiusc
▴
140
1
vote
1
reply
226
views
updated 3 days ago by
Mensur Dlakic
★
30k
•
written 3 days ago by
Ria
•
0
0
votes
0
replies
158
views
6
votes
3
replies
329
views
updated 3 days ago by
Phil Ewels
★
1.5k
•
written 4 days ago by
DdogBoss
▴
30
0
votes
1
reply
4.9k
views
0
votes
2
replies
5.5k
views
12
votes
14
replies
3.3k
views
6 follow
updated 1 day ago by
colindaven
8.0k
•
written 11 days ago by
PolenP
▴
10
1
vote
1
reply
227
views
0
votes
1
reply
236
views
updated 3 days ago by
Arup Ghosh
3.4k
•
written 4 days ago by
biotrekker
▴
110
0
votes
2
replies
1.7k
views
updated 4 days ago by
Meisam
▴
250
•
written 7.3 years ago by
spacemorrissey
▴
280
0
votes
3
replies
2.7k
views
2
votes
9
replies
3.4k
views
updated 4 days ago by
Ales
▴
70
•
written 7.3 years ago by
caggtaagtat
★
1.9k
0
votes
1
reply
212
views
0
votes
0
replies
173
views
0
votes
0
replies
178
views
4 days ago by
Physalia-courses
★
2.6k
1
vote
4
replies
411
views
updated 3 days ago by
jared.andrews07
★
19k
•
written 4 days ago by
sbissi102
▴
10
1
vote
0
replies
192
views
1
vote
4
replies
444
views
2
votes
2
replies
346
views
4
votes
4
replies
443
views
updated 5 days ago by
Mensur Dlakic
★
30k
•
written 5 days ago by
nuorain
▴
40
6
votes
6
replies
545
views
updated 4 days ago by
Rob
7.2k
•
written 5 days ago by
Assa Yeroslaviz
★
1.9k
1
vote
1
reply
294
views
0
votes
0
replies
214
views
5 days ago by
Physalia-courses
★
2.6k
0
votes
1
reply
275
views
Recent Votes
Recent Locations •
All
United States, 2 minutes ago
Australia, 32 minutes ago
United States, 44 minutes ago
UCLA, 58 minutes ago
USA, 1 hour ago
Oviedo, 1 hour ago
Heraklion, Crete, Greece, 2 hours ago
Recent Awards •
All
Teacher to
andres.firrincieli
3.9k
Teacher to
Aleksandra
▴
180
Popular Question to
vernonlim98
•
0
Popular Question to
EagleEye
7.6k
Scholar to
saamhasan55
▴
30
Recent Replies
Comment: DESeq2 on metagenome KO counts
by
ATpoint
89k
This statement makes zero sense in response to what I wrote.
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
I focused on the fact that DESeq2 models overdispersion, but overlooked its implicit assumption: normalisation works when the main source o…
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
Thank you very much. I mistakenly drew a superficial analogy. I equated KO counters with gene counters.
Comment: DESeq2 on metagenome KO counts
by
ATpoint
89k
Both vst or logcpm will favor expression level (magnitude of counts) rather than differences in a hclust/heatmap. For differences you need …
Comment: DESeq2 on metagenome KO counts
by
ATpoint
89k
The DESeq2 developer has advised many times against DESeq2 for metagenomics. Please search for related posts over at support.bioconductor.o…
Answer: DESeq2 on metagenome KO counts
by
andres.firrincieli
3.9k
I wouldn’t use DESeq2 on aggregated raw counts per KO, because doing so means accepting two pretty big assumptions:
1. All genes with t…
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
(I apologise for my previous reply; I wanted to be as detailed as possible)
DESeq2 handles KO counts perfectly well. For a time-series des…
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
I apologise for how that sounded; I wanted to be as detailed as possible.
Comment: GUI commercial software for 10x single cell gene expression analysis
by
Istvan Albert
103k
A comment on: "*Wonky analysis is possible even with open-source R libraries.*"
True - and arguably, the risk of a wonky analysis is actua…
Comment: GUI commercial software for 10x single cell gene expression analysis
by
GenoMax
154k
`Trailmaker` likely only supports Parse's evercode data. <br>
`BioTuring` does not appear to have published pricing. <br>
`Rosalind` appear…
Answer: GUI commercial software for 10x single cell gene expression analysis
by
firestar
★
1.7k
Found a few more when I asked around. I have no idea how good or bad these are. If anyone has used these or knows more about these, feel fr…
Comment: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
GenoMax
154k
> I performed a standard proteomics screening workflow
What database did you use to search against? Perhaps you are using an old (not so c…
Answer: FACS quality control based on size and doublet detection in scRNA-seq
by
ATpoint
89k
No, it's unrelated. Doublets in 10x happen if two cells get enclosed into the same GEM droplet. This has nothing to do with how flow cytome…
Answer: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
Elisabeth Gasteiger
★
2.4k
Apparently you are working with human proteins? I would strongly recommend looking only at proteins that are part of the human reference pr…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
colindaven
8.0k
Wow, thats too long. How much RAM does your machine have, it is likely swapping
Traffic: 3019 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6