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Comment: metaspades error
by
pranjalarun10
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0
Trimming was done independently for both R1 and R2 files i.e., with trimming option of trimming base pair less than 35 bp and Q less than 30
Comment: The BUSCO assessment of the MAKER annotation results shows a low completeness
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mut
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I used the BUSCO embryophyta_odb10 database and Maker v3.01.03
Comment: making estimated proteome from genome
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GenoMax
147k
> The only thing that I can think of is to use prodigal on each of the 2099 individual contigs, and then combine them later.
If it works t…
Comment: Demultiplexing inDrop dataset
by
jade
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Hello,
The page "https://iccb.med.harvard.edu/files/iccb/files/sequencing_indrops_libraries_02_28_18.pdf" cannot be found. Could you please…
Comment: Combine GBS and WGS data for variant calling in GWAS analysis
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behnam
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Thanks for your comment.
I think in your comment, you mean we should analyze the parents and population separately, then combine the cover…
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swbarnes2
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Why do you want to do this? Does Illumina even make instruments anymore where you can have one sample in one lane, and a different sample …
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by
solo.albif
•
0
I started my analysis with a single Seurat object and used `SplitObject(object, split.by = "ident")` to split by Patient ID. The problem wi…
Comment: How to include the lane index in STARsolo count matrix barcodes.tsv?
by
mk
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thanks, so how do i make starsolo report the NNNNNNNN-i index of the gem well for barcode NNNNNNNN?
Answer: How to include the lane index in STARsolo count matrix barcodes.tsv?
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If you combine two samples together, the -1 and -2 at the end of cell barcodes will matter, so you know what cells came from what samples, …
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FYI - this didn't get tagged as miloR, so it didn't come up on my radar.
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Similar analyses have been published before which should help you get started .
https://onlinelibrary.wiley.com/doi/pdf/10.1002/ctm2.971<b…
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GenoMax
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> any tips on where to find reliable reference genomes for HPV and EBV would be helpful.
Search here: https://www.ncbi.nlm.nih.gov/dataset…
Comment: Can I combine variants called using T2T-CHM13 with GRCh38?
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i would be alarmed if new stuff was showing up in exomes. it is an interesting idea though.
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I would keep the two variants separate. Each variant call process may have problems, though those called for T2T would have fewer errors ar…
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the error message seems very clear about the problem:
Your chromosome names do not match.
You show a lot of code above, but what are the…
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