2 hours ago by
Peter
•
0
0
votes
0
replies
32
views
0
votes
0
replies
49
views
2
votes
2
replies
136
views
0
votes
2
replies
105
views
1
vote
2
replies
155
views
2
votes
2
replies
139
views
839
votes
170
replies
179k
views
112 follow
updated 6 weeks ago by
Biostar
3.6k
•
written 8.5 years ago by
Istvan Albert
102k
0
votes
0
replies
89
views
0
votes
0
replies
66
views
0
votes
0
replies
65
views
20 hours ago by
Begonia_pavonina
▴
210
5
votes
2
replies
152
views
16 hours ago by
Mahdi&Christ
▴
20
3
votes
4
replies
245
views
9
votes
9
replies
405
views
0
votes
1
reply
137
views
0
votes
1
reply
619
views
2
votes
10
replies
372
views
updated 6 hours ago by
GenoMax
151k
•
written 1 day ago by
anthony.santana.703.j
▴
30
0
votes
0
replies
105
views
1 day ago by
Claire Watson
▴
60
1
vote
6
replies
257
views
3
votes
0
replies
117
views
2
votes
0
replies
106
views
2
votes
0
replies
105
views
2
votes
0
replies
123
views
0
votes
2
replies
158
views
2
votes
7
replies
340
views
updated 1 day ago by
GenoMax
151k
•
written 2 days ago by
ramiro.barrantes
▴
60
1
vote
1
reply
143
views
0
votes
1
reply
141
views
0
votes
1
reply
141
views
1
vote
1
reply
185
views
1
vote
1
reply
172
views
0
votes
2
replies
173
views
1
vote
1
reply
303
views
updated 2 days ago by
colindaven
7.6k
•
written 5 days ago by
emil.paulitz
•
0
0
votes
0
replies
147
views
0
votes
1
reply
193
views
0
votes
0
replies
185
views
0
votes
1
reply
521
views
0
votes
0
replies
190
views
3 days ago by
Physalia-courses
★
2.6k
0
votes
4
replies
2.1k
views
1
vote
0
replies
194
views
0
votes
1
reply
263
views
updated 4 days ago by
GenoMax
151k
•
written 4 days ago by
michael.dhar
•
0
2
votes
4
replies
1.1k
views
0
votes
2
replies
340
views
0
votes
0
replies
224
views
8
votes
5
replies
483
views
updated 4 days ago by
GenoMax
151k
•
written 5 days ago by
sarahmanderni
▴
130
0
votes
0
replies
235
views
0
votes
1
reply
288
views
0
votes
0
replies
216
views
5
votes
4
replies
703
views
0
votes
1
reply
264
views
2
votes
2
replies
1.9k
views
4
votes
2
replies
339
views
4 days ago by
ATS
▴
10
Recent Votes
Recent Locations •
All
Australia, just now
France, 1 minute ago
Italy, 5 minutes ago
Pakistan, 5 minutes ago
Norway, 5 minutes ago
United Kingdom, 7 minutes ago
Germany, 9 minutes ago
Recent Awards •
All
Teacher to
AshleeThomson
▴
130
Popular Question to
Binghong
▴
20
Popular Question to
analyst
▴
70
Popular Question to
Dunois
★
2.9k
Popular Question to
lessismore
★
1.4k
Popular Question to
Jeremy Leipzig
23k
Recent Replies
Comment: Time scaled tree with IQTree giving off looking branch length
by
SushiRoll
▴
140
Hey cfos! Thanks for the reply! I did apply the ascertainment correction model, in fact I applied GTR+F+ASC for this tree. I'm wondering if…
Comment: Annotation Issue using miRBASE gff
by
GenoMax
151k
That is probably fine since you are counting only the miRNA using that GTF. You already compressed the data by desuplicating UMI. Take a lo…
Comment: Annotation Issue using miRBASE gff
by
anthony.santana.703.j
▴
30
Hello Geno,
I figured out part of my issue, my gtf file was corrupt, and I also changed my featureCounts params:
featureCounts -F GTF…
Answer: Best Tool for Fast RNA-Seq Transcriptome Alignment to Extract Unmapped Reads
by
dsull
★
7.6k
Honestly, I'd just stick with `STAR` for this purpose. You can use the `--outReadsUnmapped` option to directly output the unmapped reads in…
Answer: Time scaled tree with IQTree giving off looking branch length
by
cfos4698
★
1.1k
Did you remove invariant (constant) sites from the SNP alignment? If so, you should probably also be specifying an ascertainment bias corre…
Answer: Best Tool for Fast RNA-Seq Transcriptome Alignment to Extract Unmapped Reads
by
GenoMax
151k
> my guess is this would be cheaper/faster than just aligning all of them to the genome to begin with
If you are paying for all of your co…
Comment: Annotation Issue using miRBASE gff
by
anthony.santana.703.j
▴
30
Thank you but, didnt make a difference, let me see what I can find, there has to be a reason.
Comment: Signature Matrix Modifications/Subclustering
by
MitchT
▴
10
Thank you. Sorry, I should have prefaced that I was able to find canonical markers of these cells in my main cluster, which is a mixture of…
Comment: Error Scaffolding with RagTag
by
Michael
55k
>Troubleshooting Steps: Ran minimap2 manually on Pritchett’s reference (attraktion.fasta) and query (pt2_busco.fa); it generated a 442 MB .…
Comment: Identify transgene insert loci/copy number in nanopore De-novo assembly
by
ctmfarland
▴
20
That was an excellent idea. I used blastn to pull out all ONT reads containing some/all of the gene of interest and mapped that to the refe…
Comment: Annotation Issue using miRBASE gff
by
GenoMax
151k
Looking at the alignment file we know the reference chromosome names match the GTF from miRBase. So that should be fine. Your reads are als…
Comment: Annotation Issue using miRBASE gff
by
anthony.santana.703.j
▴
30
samtools view:
LH00150:566:22TM3VLT3:6:2196:18797:12321_CTTTTCTGCCTA 16 chr1 10571 255 16M * 0 0 …
Comment: Picard CollectRawWgsMetrics runs much slower on non-Apple chip machine
by
Michael
55k
"Wow those M-chips really are fast!" possibly this. Especially with the M4 (essentially a laptop chip) when it comes to single thread perfo…
Comment: Error Scaffolding with RagTag
by
GenoMax
151k
If the run stops at the same point in the pritchett file then you should examine that to see if there is any data corruption. Make sure the…
Comment: Error Scaffolding with RagTag
by
Bipin
▴
10
There was nothing on SLURM logs, the error mentioned above is in the error file. For Madsen, log file looks similar but is complete. For Pr…
Traffic: 3125 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6