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120,506 results • Page
1 of 2411
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Votes
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0
votes
1
reply
17
views
Determine binding sites in unstimulated TF ChIP-seq samples
ChIP-seq
csaw
updated 47 minutes ago by
ATpoint
87k • written 52 minutes ago by
Jacek
▴ 20
0
votes
0
replies
39
views
GO analysis custom reference?
analysis
GO
2 hours ago by
Pit
• 0
0
votes
2
replies
42
views
Query on WES
WES
8 minutes ago by
rauf.sania786
• 0
4
votes
12
replies
6.1k
views
8 follow
BLAST Database error: Database memory map file error
makeblastdb
updated 3 hours ago by
vernisara
• 0 • written 5 months ago by
Martina
• 0
0
votes
0
replies
37
views
News:
Introduction to Machine Learning in R online course
R
statistics
AI
machinelearning
4 hours ago by
Transmitting Science communication
▴ 70
828
votes
169
replies
168k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 5 months ago by
Biostar
3.4k • written 8.2 years ago by
Istvan Albert
102k
0
votes
1
reply
50
views
RNA variant calling - SNP and indels files
mouse
variant_calling
genomics
RNA
updated 49 minutes ago by
1769mkc
★ 1.2k • written 5 hours ago by
Nerelys
• 0
3
votes
4
replies
214
views
Nanopore long-read sequencing doubts and problems
minmap2
long-reads
NanoPlot
Nanopore
42 minutes ago by
ferran.llobet
▴ 10
0
votes
0
replies
55
views
Query Regarding Missing Sample Paths in Graph-Based Pangenome Construction
pangenome
variant_calling
giraffe
vg
haplotypes
11 hours ago by
s_135
▴ 10
1
vote
3
replies
183
views
cannot install GO.db in windows
GO.db
updated 5 hours ago by
i.sudbery
21k • written 20 hours ago by
Naila
• 0
1
vote
5
replies
201
views
conda :FileNotFoundError: [Errno 2] No such file or directory
conda
updated 5 hours ago by
Mensur Dlakic
★ 29k • written 22 hours ago by
JieQY
• 0
5
votes
5
replies
199
views
Samtools view only showing read2 for PE mapped reads
Samtools
mem2
bwa
flagstat
mapping
updated 23 hours ago by
Pierre Lindenbaum
165k • written 1 day ago by
knickknack
• 0
0
votes
3
replies
203
views
Color AlphaFold PDB by B-Factor (pLDDT) in PyMol
pdb
pymol
updated 22 hours ago by
Mensur Dlakic
★ 29k • written 1 day ago by
Schmoho
▴ 10
2
votes
2
replies
174
views
How to Resolve Overlapping Peaks in Sanger Chromatograms?
sequence-analysis
sanger-sequencing
dna-sequencing
chromatogram
19 hours ago by
JOSÉ
• 0
4
votes
7
replies
270
views
Manual conversion of VCF genotypes to nucleotide sequence
vcf
nucleotide
sequence
updated 21 hours ago by
Ram
44k • written 1 day ago by
endretoth
▴ 40
2
votes
3
replies
278
views
How to remove rows with empty gene symbols from a seurat object?
seurat
updated 1 day ago by
Michael
55k • written 3 days ago by
Chris
▴ 360
4
votes
11
replies
550
views
7 follow
Phylogenetic tree from whole genome using BUSCO
BUSCO
whole_genome
phylogenetic_tree
updated 2 days ago by
colindaven
7.2k • written 5 days ago by
manaswiniparija3
▴ 60
0
votes
0
replies
112
views
News:
Course on Ancient Metagenomics - online, 27-29 May
ancientDNA
Metagenomics
aMeta
2 days ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
620
views
Difference between trim galore report and Fastqc pre trimming
trim-galore
updated 21 hours ago by
Ram
44k • written 2.6 years ago by
Luern
• 0
0
votes
0
replies
144
views
From Cram to gVCF
Cram
gVCF
2 days ago by
heureuse
• 0
2
votes
4
replies
1.1k
views
R CytoTRACE2 Results: Potency Score of Naive B Cells Lower Than Memory B and Plasmablasts – Contradicts Biological Knowledge
CytoTRACE2
2 days ago by
Binghong
▴ 20
3
votes
1
reply
299
views
Rules for Assigning Start and End Phase to Coding Exons
stare_phase
reading_frame
end_phase
updated 2 days ago by
Jorge - Ensembl
▴ 10 • written 5 days ago by
Milica
▴ 20
0
votes
1
reply
167
views
converting transcript positions to genomic locations
genomic
locations
updated 2 days ago by
Pierre Lindenbaum
165k • written 2 days ago by
baibhu1234
▴ 20
0
votes
1
reply
201
views
Read groups RNA-seq and hisat2
hisat2
RNA-seq
read-groups
updated 2 days ago by
Ram
44k • written 3 days ago by
frarodmar17
• 0
0
votes
0
replies
143
views
simulate a genome with specific event
simulation
genome
retrocopy
3 days ago by
Matteo Ungaro
▴ 110
6
votes
4
replies
776
views
6 follow
Help with R programming assignment
R
offtopic
updated 1 day ago by
Michael
55k • written 7 months ago by
sidhanthbaru
• 0
0
votes
0
replies
141
views
HOMER findMotifsGenome.pl failed compilation
findMotifsGenome.pl
motifanalysis
HOMER
3 days ago by
India
• 0
1
vote
1
reply
226
views
Job:
SciLifeLab PULSE: Program for Future Leaders in Life Science (48 positions!)
Entrepreneur
Sweden
Mentoring
Postdoctoral
Leadership
3 days ago by
Matthias Zepper
5.0k
1
vote
1
reply
225
views
MSA with extended alphabet
Multiple
sequence
MSA
alignment
updated 3 days ago by
Mensur Dlakic
★ 29k • written 3 days ago by
retzl.bernhard
• 0
0
votes
0
replies
140
views
Help with Finding SNPs in H. pylori Assembled Genomes
question
Technical
3 days ago by
vrao
• 0
0
votes
2
replies
222
views
Reference Genome with decoy for RNAseq data processing
STAR
SALMON
RNAseq
updated 2 days ago by
Zhenyu Zhang
★ 1.2k • written 3 days ago by
cwwong13
▴ 40
1
vote
1
reply
205
views
multiple ATG SEQUENCE
MULTIPE
sequence
ATG
offtopic
updated 3 days ago by
Carlo Yague
8.9k • written 3 days ago by
Sijjil
• 0
0
votes
0
replies
156
views
Keep SNP's with call rate greater than 30 in VCF
SNP
call
rate
ROH
genotyping
updated 2 days ago by
Bastien Hervé
6.1k • written 3 days ago by
Smilesky
• 0
9
votes
10
replies
5.3k
views
7 follow
Buy PC for metagenomics
Assembly
alignment
genome
next-gen
updated 3 days ago by
Joe
22k • written 6.6 years ago by
lugonauta
▴ 10
0
votes
0
replies
147
views
Dynamic tree cut results
clustering
DEG
2 days ago by
Mai.Nabil
• 0
0
votes
0
replies
150
views
Significant variation in FastQ File Sizes - WES
LibraryPrep
WES
Human
3 days ago by
irate.pirate
• 0
0
votes
2
replies
334
views
Repetitive sequences analysis
Repetitive
sequences
updated 3 days ago by
rfran010
★ 1.4k • written 4 days ago by
frarodmar17
• 0
0
votes
1
reply
216
views
Protein secuence extraction from gpff file for a genes list
Protein
gpff
updated 3 days ago by
JC
13k • written 3 days ago by
reza
▴ 300
0
votes
0
replies
174
views
Job:
Postdoctoral Researcher in Single-Cell Sequencing Analysis
researcher
post-doctoral
3 days ago by
ejvandenoord
• 0
0
votes
2
replies
283
views
filtering heterozygous genotypes
bcftools
heterozygous
3 days ago by
analyst
▴ 60
2
votes
0
replies
235
views
An alternative to haplotype phasing - assembly?
samtools
assembly
BAM
spades
phasing
3 days ago by
Jess
▴ 60
0
votes
0
replies
158
views
how to make/get the SNP annotation file for running TORUS
TORUS
Annotation
SNP
GWAS
updated 3 days ago by
GenoMax
149k • written 3 days ago by
lee
• 0
1
vote
1
reply
215
views
Flongle 16srRNA nanopore sequencing
R.9.4.1
flow
flongle
updated 3 days ago by
GenoMax
149k • written 3 days ago by
Major ginger
• 0
1
vote
2
replies
274
views
Genomes with existing T2T assemblies?
T2T
genome
assembly
reference
updated 4 days ago by
cmdcolin
★ 4.1k • written 4 days ago by
predeus
★ 2.1k
1
vote
2
replies
313
views
Installing ggpubr in Docker container rocker/r-base??
ggpubr
dockerfile
docker
r
updated 3 days ago by
Ram
44k • written 4 days ago by
giulia.trauzzi
▴ 30
0
votes
0
replies
160
views
News:
Bayesian Data Analysis in R course - online, March 3-7
Bayesian
Statistics
R
4 days ago by
Physalia-courses
★ 2.6k
4
votes
5
replies
378
views
Should we cite sed / awk in papers?
sed
awk
updated 3 days ago by
ATpoint
87k • written 4 days ago by
amy__
▴ 250
0
votes
0
replies
152
views
Briggs model parametes
Briggs
damage
Gargammel
4 days ago by
anna.utili
• 0
3
votes
7
replies
485
views
Nanopore sequencing - plasmid assembly length confusion
assembly
long
nanopore
reads
3 days ago by
abedkurdi10
▴ 190
0
votes
0
replies
173
views
Post-GWAS colocalization analysis VS. variant annotation, what's the difference?
variant
annotation
snp
colocalization
GWAS
4 hours ago by
sarfdezco
• 0
120,506 results • Page
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Answer: convert sam into paf format
Answer: Nanopore long-read sequencing doubts and problems
Answer: Nanopore long-read sequencing doubts and problems
miRNA with UMI - any papers showing this improves accuracy?
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A: When Aligning Mirna (Micro Rna) What Reference Is Better? (Whole Genome Or Mirba
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Recent Replies
Comment: Query on WES
by
rauf.sania786
• 0
thank you
Comment: Nanopore long-read sequencing doubts and problems
by
ferran.llobet
▴ 10
Hi @jc, Thank you for your answer. Yes, I do see these fields in the BAM files that I have. Then, just another quick question. So the B…
Comment: Query on WES
by
ATpoint
87k
Please use this button and upload the image. ![enter image description here][1] [1]: /media/images/9e5ab5ec-f0b4-4897-b527-95c54307
Comment: Determine binding sites in unstimulated TF ChIP-seq samples
by
ATpoint
87k
Ignore the input -- it is only used for peak calling but not differential analysis. The comparison between untreated is technically the sam…
Comment: RNA variant calling - SNP and indels files
by
1769mkc
★ 1.2k
what the error log just in case if you are not getting desired output?
Answer: BLAST Database error: Database memory map file error
by
vernisara
• 0
Goodrmorning everyone, I have the same problem, I am trying to built a local BLAST for nucleotide and I downloaded all the .nt file with t…
Comment: conda :FileNotFoundError: [Errno 2] No such file or directory
by
Mensur Dlakic
★ 29k
There are many things that can be tried to fix this problem, so it is a matter of reading through all of them: 1) convert the format on the…
Comment: cannot install GO.db in windows
by
i.sudbery
21k
My memory from having R installed in windows (and it has been a long time) is that the library is generally kept in "My Documents". What is…
Comment: conda :FileNotFoundError: [Errno 2] No such file or directory
by
JieQY
• 0
I used `wget https://repo.anaconda.com/archive/Anaconda3-2024.10-1-Linux-x86_64.sh` to download anaconda, and the above error occurred dur…
Comment: conda :FileNotFoundError: [Errno 2] No such file or directory
by
JieQY
• 0
Thank you very much for your reply! Yes, as you suggested, I checked the error messages on google and github [https://github.com/conda/co…
Answer: Nanopore long-read sequencing doubts and problems
by
colindaven
7.2k
I use this command to map ONT reads - which as JC says have previously had the methylation called during basecalling with Dorado. - Note …
Comment: Why do people say it doesn't matter whether you run unassembled or assembled rea
by
trkfs
▴ 10
Thanks for your help!
Answer: cannot install GO.db in windows
by
Mensur Dlakic
★ 29k
> Please can someone help me. i have tried everything but it won't install I have also changed the environmental variable It would be im…
Answer: Nanopore long-read sequencing doubts and problems
by
JC
13k
if the nanopore data was called with a recent version of dorado, you can have methylation calls in your BAM file (MM and ML fields), you ca…
Comment: How to Resolve Overlapping Peaks in Sanger Chromatograms?
by
JOSÉ
• 0
Thank you very much for your suggestions and for the clarity in your explanation. The tips on using acrylamide gel, restriction enzyme dige…
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