47 minutes ago by
aiswaryabioinfo
▴
30
0
votes
0
replies
13
views
0
votes
3
replies
43
views
updated 1 hour ago by
i.sudbery
21k
•
written 2 hours ago by
paguirreazorin
•
0
0
votes
0
replies
34
views
updated 2 hours ago by
Nicolas Rosewick
11k
•
written 3 hours ago by
AIMAR
•
0
7
votes
5
replies
585
views
0
votes
0
replies
34
views
3 hours ago by
rahma.khoualdia
•
0
835
votes
169
replies
170k
views
110 follow
updated 6 months ago by
Biostar
3.4k
•
written 8.3 years ago by
Istvan Albert
102k
0
votes
2
replies
90
views
updated 1 hour ago by
theHumanBorch
▴
240
•
written 5 hours ago by
txema.heredia
▴
210
4
votes
12
replies
6.9k
views
12 follow
updated 5 hours ago by
5heikki
11k
•
written 10.3 years ago by
lyz10302012
▴
480
0
votes
1
reply
65
views
updated 6 hours ago by
colindaven
7.3k
•
written 7 hours ago by
analyst
▴
60
0
votes
0
replies
47
views
2
votes
3
replies
135
views
updated 8 hours ago by
GenoMax
150k
•
written 14 hours ago by
nigussie.amu
•
0
0
votes
0
replies
46
views
0
votes
1
reply
73
views
0
votes
0
replies
55
views
updated 12 hours ago by
Ram
45k
•
written 13 hours ago by
EvoGenExplorer
•
0
2
votes
3
replies
161
views
updated 12 hours ago by
Jouni Sirén
▴
580
•
written 19 hours ago by
Sauers
▴
10
8
votes
16
replies
395
views
6 follow
0
votes
1
reply
125
views
0
votes
1
reply
143
views
updated 20 hours ago by
Adrian Altenhoff
★
1.1k
•
written 1 day ago by
nmalexan
•
0
0
votes
0
replies
68
views
1
vote
2
replies
138
views
0
votes
3
replies
130
views
updated 23 hours ago by
ATpoint
87k
•
written 23 hours ago by
pairedttest
▴
30
1
vote
5
replies
310
views
6 follow
0
votes
0
replies
63
views
23 hours ago by
kristina
•
0
3
votes
0
replies
93
views
0
votes
0
replies
74
views
updated 23 hours ago by
Ram
45k
•
written 1 day ago by
abhijitswain
•
0
1
vote
1
reply
246
views
3
votes
2
replies
196
views
3
votes
6
replies
277
views
0
votes
0
replies
92
views
0
votes
0
replies
94
views
0
votes
3
replies
198
views
2
votes
4
replies
336
views
updated 1 day ago by
Istvan Albert
102k
•
written 3 days ago by
Megan
▴
50
2
votes
4
replies
233
views
9 hours ago by
Meghan.T
•
0
15
votes
3
replies
1.1k
views
0
votes
1
reply
132
views
0
votes
1
reply
134
views
updated 1 day ago by
Istvan Albert
102k
•
written 1 day ago by
bioinfo
▴
150
2
votes
3
replies
227
views
1
vote
2
replies
199
views
updated 1 day ago by
Jeremy Leipzig
23k
•
written 2 days ago by
mary.v.volkova
▴
20
0
votes
2
replies
147
views
22 hours ago by
carolofharvest
▴
50
1
vote
2
replies
854
views
0
votes
1
reply
143
views
updated 1 day ago by
Ram
45k
•
written 2 days ago by
hellothere
•
0
0
votes
2
replies
164
views
0
votes
1
reply
179
views
updated 2 days ago by
i.sudbery
21k
•
written 2 days ago by
baibhu1234
▴
40
0
votes
0
replies
130
views
2 days ago by
mahlet.teka
•
0
0
votes
0
replies
251
views
updated 2 days ago by
Pierre Lindenbaum
165k
•
written 2 days ago by
1phamcha2
•
0
2
votes
2
replies
225
views
1 day ago by
omicon
▴
40
1
vote
0
replies
125
views
0
votes
1
reply
211
views
0
votes
1
reply
187
views
3
votes
2
replies
256
views
Recent Votes
Recent Locations •
All
United Kingdom, 1 minute ago
India, 2 minutes ago
Germany, 2 minutes ago
United States, 4 minutes ago
France, 4 minutes ago
Germany, 6 minutes ago
France, 7 minutes ago
Recent Awards •
All
Popular Question to
aLex97
•
0
Popular Question to
shelkmike
★
1.5k
Scholar to
Devon Ryan
105k
Popular Question to
Nelo
▴
20
Popular Question to
emalekos
▴
40
Popular Question to
dthorbur
★
2.9k
Popular Question to
graeme.thorn
▴
110
Recent Replies
Answer: Higher number of reads mapped to transcriptome vs genome in star mapping
by
i.sudbery
21k
I can think of a possibility
Consider:
You aligned reads to transcriptome, that contains two isoforms for a gene. Reads that come fro…
Comment: Higher number of reads mapped to transcriptome vs genome in star mapping
by
i.sudbery
21k
You might also try seeing how many alignments to each before deduplication. And also looking at how many *reads" there are, rather than how…
Comment: Higher number of reads mapped to transcriptome vs genome in star mapping
by
i.sudbery
21k
Can you clarify what you mean by "isoform selection"?
Comment: Tool for automatic immune cell annotation in MOUSE?
by
theHumanBorch
▴
240
When annotating, I like to combine canonical markers with automated annotation systems - the go to for mouse immune populations I use is
1…
Answer: AliNe (Alignment in Nextflow)
by
Juke34
9.2k
`Last`is now present in AliNe v1.3.0
Before that `bwa-mem2` and `salmon` have been added in AliNe v1.2.0
Comment: prokka with too many "hypothetical proteins" and genome comparison
by
avinci1
▴
20
Thank you! I will try that!
Comment: prokka with too many "hypothetical proteins" and genome comparison
by
avinci1
▴
20
There are 64 contigs for one genome and 114 for the other one.
The sequencing service should be reputable.
I obtained the same output with …
Comment: How I can curate all exon start-end positions for 50 genes (to create GTF file)?
by
Esra
•
0
Thank you for your answee.I also looked these AGAT tools you suggested, but I wasn't sure how to use it (I am actually a medical doctor and…
Comment: How I can curate all exon start-end positions for 50 genes (to create GTF file)?
by
Esra
•
0
Thank you for your reply and for the clarification. Yes, I wanted to curate the MANE Select (Canonical) transcript's exons, so thanks for t…
Comment: Tool for automatic immune cell annotation in MOUSE?
by
Bastien Hervé
6.1k
Look at the gene markers driving each cluster to see if you can identify specific cell population. You can also fetch from online databases…
Comment: Identifying primer sequences from raw FASTQ files
by
michael.ante
★
4.0k
The primers don't need to be unambiguous sequences. Often to improve binding, they are given with ambiguous DNA-letters, which in the end m…
Comment: merging diploid and tetraploid vcfs
by
colindaven
7.3k
How are you calling tetraploid variants out of interest ?
Answer: How to draw venn diagram for two CNV lists?
by
5heikki
11k
[This][1] is my favorite online tool for list comparisons
[1]: https://molbiotools.com/listcompare.php
Comment: Recommended workflow to pre-process and demultiplex scRNAseq data based on the H
by
mete.han.celebi
•
0
Thank you very much! Yes I haven't seen this. That is very valuable!
Traffic: 2320 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6