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121,001 results • Page
1 of 2421
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Rank
Views
Votes
Replies
0
votes
3
replies
546
views
QIAseq 16S/ITS Screening Panel Primers?
16s
primers
metagenomics
ITS
updated 55 minutes ago by
GenoMax
150k • written 3.2 years ago by
Isaac
• 0
0
votes
1
reply
92
views
Analysing gene CNV from TCGA using TCGAbiolinks
TCGA
updated 1 hour ago by
Zhenyu Zhang
★ 1.2k • written 1 day ago by
Matt
• 0
0
votes
3
replies
130
views
How to use LOEUF plugin in VEP?
LOEUF
VEP
updated 3 hours ago by
GenoMax
150k • written 19 hours ago by
Sd
• 0
0
votes
0
replies
45
views
Trouble with CIBERSORTx: All P-values > 0.05, Low Correlation, High RMSE – What Could Be Going Wrong?
R
CIBERSORTx
CIBERSORT
script
updated 3 hours ago by
GenoMax
150k • written 4 hours ago by
Pumla
• 0
0
votes
1
reply
51
views
How can I identify genes with the MSTRG notation?
R
stringtie
RNA-Seq
updated 4 hours ago by
GenoMax
150k • written 4 hours ago by
pinheirofabiano
▴ 110
0
votes
0
replies
73
views
dittoHeapmap didn't work in my data set(error code :Error in .subscript.2ary(x, i, j, drop = TRUE) : subscript out of bounds)
dittoseq
scRNAseq
updated 1 hour ago by
ATpoint
87k • written 5 hours ago by
kayah
▴ 20
836
votes
167
replies
173k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 4 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
0
votes
2
replies
74
views
Visualise duplications and inversions
inversions
duplications
updated 3 hours ago by
Wayne
★ 2.1k • written 6 hours ago by
priya.bmg
▴ 70
1
vote
1
reply
72
views
Exon-level counts via featureCounts
diffSpliceDGE
exon
RNASeq
featureCounts
edgeR
updated 3 hours ago by
GenoMax
150k • written 6 hours ago by
n_navy
• 0
0
votes
5
replies
139
views
Picard : Bait_interval and Target_interval
read_depth_measure
Picard_tool
Bed_file
coverage
3 hours ago by
AIMAR
• 0
3
votes
2
replies
130
views
Does vg giraffe output secondary and supplimentary alignments?
vg
giraffe
13 hours ago by
lushjia
• 0
0
votes
0
replies
82
views
Question about Calculation of Dietary index in the NHANES Database
index
DI-GM
Dietary
NHANES
1 day ago by
城玮
• 0
0
votes
1
reply
170
views
Detection SNPs and Indels Bacteria
SNPs
updated 1 day ago by
Istvan Albert
102k • written 1 day ago by
Eltercertopico
• 0
1
vote
4
replies
341
views
protease candidates for my protein of interest
cleaving-site
proteases
protein
1 day ago by
Fatemeh
▴ 40
0
votes
1
reply
132
views
Different number of fragments after doublets removal in each run
ArchR
updated 1 day ago by
Bastien Hervé
6.2k • written 1 day ago by
Bioinformatics_16
• 0
0
votes
0
replies
94
views
Seeking Guidance on Next Steps for DNA Methylation Biomarker Screening in Cancer Prognosis
early-cancer-screening
TCGA
Epigenomics
DNA-Methylation
cancer-prognosis
1 day ago by
Riley J
• 0
0
votes
1
reply
147
views
Help with Panel of Normal (PON)
somatic-mutation
PoN
updated 1 day ago by
Ram
45k • written 1 day ago by
TJ
• 0
4
votes
2
replies
242
views
Why don't I see people calculating FPKM from normalized read counts?
normalization
RNA-seq
updated 1 day ago by
ATpoint
87k • written 2 days ago by
shelkmike
★ 1.5k
1
vote
7
replies
377
views
Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-genomes).
EPI2ME
MEDAKA
updated 1 day ago by
GenoMax
150k • written 2 days ago by
Anurag
• 0
0
votes
0
replies
89
views
freebayes with pools of different number of individuals
freebayes
poolseq
1 day ago by
Jeanlain
• 0
0
votes
3
replies
194
views
Insight into Trimmomatic
Trimmomatic
Trimming
Reads
updated 1 day ago by
GenoMax
150k • written 1 day ago by
jh5ym
• 0
1
vote
3
replies
220
views
Trouble when uploading FASTQ files on NCBI
rnaseq
ncbi
10 hours ago by
Gerard
▴ 20
1
vote
4
replies
248
views
SyntaxError in file /snakefile, line 22: invalid decimal literal: None
python
snakemake
updated 1 day ago by
Jesse
▴ 870 • written 2 days ago by
Ana
▴ 10
3
votes
2
replies
247
views
News:
Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcome!
mmcif
2 days ago by
Shravya
• 0
0
votes
0
replies
127
views
News:
Course: Introduction to Genome Annotation (Online, 19–22 May 2025)
Gene-Prediction
Genome-Annotation
Gene-Models
2 days ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
160
views
Error with running cellranger-atac count
cellranger
atac
single-cell
updated 1 day ago by
Ram
45k • written 2 days ago by
Khôi
• 0
0
votes
2
replies
225
views
HIC-pro with Forward and reverse reads not paired even when I use their test data.
HIC-pro
1 day ago by
Aki
▴ 20
1
vote
1
reply
330
views
Using DEXseq on kallisto tsv outputs ?
alignement
DEXseq
kallisto
updated 2 days ago by
Gordon Smyth
★ 7.9k • written 8 days ago by
Gerard
▴ 20
0
votes
0
replies
173
views
Job:
2025 Arrowhead AI/LLM for Computational Biology and Data Science Internship
intern
updated 2 days ago by
GenoMax
150k • written 2 days ago by
Shicheng Guo
★ 9.6k
1
vote
2
replies
521
views
ChIP-seq gene blacklist for Rattus norvegicus
rat
blacklist
ChIP-seq
updated 2 days ago by
GenoMax
150k • written 12 months ago by
Thomas
• 0
4
votes
10
replies
852
views
6 follow
Extract length of sample sequence from vcf
microsatellites
bcftools
vcf
indels
variants
updated 3 days ago by
cmdcolin
★ 4.2k • written 13 days ago by
jahnreinhard.ringger
• 0
0
votes
0
replies
156
views
Tutorial:
Fast SCENIC GRN Analysis with RegDiffusion
GRN
network
scRNA-seq
wgcna
3 days ago by
Hao
▴ 10
2
votes
3
replies
625
views
Is it reasonable to do "Gene Regulatory Network Inference" for each cell type and do differential GRN
GRN
scRNA-seq
updated 3 days ago by
Hao
▴ 10 • written 2.6 years ago by
shangguandong1996
▴ 30
1
vote
2
replies
385
views
Blastx issue
blastx
lncRNA
updated 1 day ago by
Ram
45k • written 5 days ago by
Avik
• 0
2
votes
9
replies
594
views
TPM vs FPKM divergence at high values
tpm
fpkm
normalisation
updated 2 days ago by
Istvan Albert
102k • written 5 days ago by
megancgrdnr
• 0
3
votes
0
replies
175
views
Herald:
The Biostar Herald for Monday, April 14, 2025
herald
3 days ago by
Biostar
3.5k
1
vote
2
replies
324
views
What is the best way to know if a pair of genes is random?
go-terms
genes
updated 1 day ago by
Ram
45k • written 6 days ago by
Ana
▴ 10
1
vote
1
reply
263
views
How to save the AlphaFold PAE plot as an image in ChimeraX using command line ?
PAE
ChimeraX
AlphaFold
updated 3 days ago by
LauferVA
4.7k • written 5 days ago by
Picasa
▴ 680
1
vote
0
replies
146
views
News:
LAST CALL - Bioinformatics Pipeline Development with Nextflow - May 19-21, 2025 (online) - Apply Now
nextflow
rnaseq
workshop
3 days ago by
ecSeq Bioinformatics
▴ 20
1
vote
2
replies
274
views
RNA-seq, three comparisons - stick to one linear model, or use separate models?
statistics
mixed-effect-model
stats
RNAseq
linear-model
updated 1 day ago by
Ram
45k • written 3 days ago by
noodlejackson
▴ 40
1
vote
1
reply
204
views
Using Principal Components may muddle the detecting power of a GWAS?
q-value
covariates
genomic-inflation
pca
gwas
updated 3 days ago by
DBScan
▴ 480 • written 3 days ago by
AndrMod
• 0
0
votes
0
replies
157
views
Use Seed or Full Alignments from Pfam HMM as a filter step
hmm
sequences
metagenomic
3 days ago by
Janelyn123
• 0
3
votes
4
replies
337
views
Helicobacter pylori low-rate alignment
RNA-seq
transcriptomics
updated 1 day ago by
Ram
45k • written 4 days ago by
Ariadna
▴ 20
14
votes
3
replies
11k
views
Can The Wget Command Be Used To Download Pdbs
pdb
updated 3 days ago by
Calvin
• 0 • written 13.5 years ago by
Harpalss
▴ 310
0
votes
3
replies
361
views
Is a log2 transformation an essential step in preparing expression data for machine learning?
Machine-learning
preproccessing
microarray
updated 1 day ago by
Ram
45k • written 4 days ago by
Sib
▴ 60
0
votes
2
replies
932
views
How to re-annotate the structure of gene against a known genomic file
RNA-Seq
updated 4 days ago by
Mensur Dlakic
★ 29k • written 5.9 years ago by
Kanoo
• 0
0
votes
2
replies
303
views
ONT Live-Basecalling Issue?
ONT
minKNOW
live-basecalling
updated 2 days ago by
LauferVA
4.7k • written 4 days ago by
Akshay
• 0
3
votes
4
replies
351
views
Identify gene from sequence at scale in non-model organisms
sequence
gene
updated 1 day ago by
Ram
45k • written 5 days ago by
matthewmerkin32
• 0
0
votes
0
replies
206
views
News:
Cellosaurus release 52 is available
biocuration
database
knowledgebase
cell-line
updated 1 day ago by
Ram
45k • written 5 days ago by
Amos Bairoch
▴ 130
0
votes
2
replies
339
views
WGCNA
WGCNA
updated 1 day ago by
Ram
45k • written 6 days ago by
香
• 0
121,001 results • Page
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Answer: Exon-level counts via featureCounts
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
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Recent Replies
Answer: QIAseq 16S/ITS Screening Panel Primers?
by
GenoMax
150k
From `Resources Tab` --> `Application Note` found at --> https://www.qiagen.com/us/products/next-generation-sequencing/metagenomics/targete…
Comment: QIAseq 16S/ITS Screening Panel Primers?
by
ATpoint
87k
Contact the support of the company, as usual when it is about commercial products.
Answer: Analysing gene CNV from TCGA using TCGAbiolinks
by
Zhenyu Zhang
★ 1.2k
I can not answer your TCGAbiolinks question, but I can answer your GDC CNV data question. There are two types of segment - float number, …
Comment: QIAseq 16S/ITS Screening Panel Primers?
by
microecologist93
▴ 20
Hello! Any luck figuring this out? I am now facing the same situation.
Comment: benefit of DNA assembly
by
Ram
45k
This post looks like it was generated using an LLM such as ChatGPT. If that is the case, please mention it in the comment. LLM generated co…
Comment: Picard : Bait_interval and Target_interval
by
AIMAR
• 0
Yes the genomic position like for the first line are not the same.
Answer: How to use LOEUF plugin in VEP?
by
GenoMax
150k
Looks like that file is available from Nature: https://static-content.springer.com/esm/art%3A10.1038%2Fs41586-020-2308-7/MediaObjects/41586…
Comment: Visualise duplications and inversions
by
Wayne
★ 2.1k
[SVbyEye](https://github.com/daewoooo/SVbyEye) says it handles duplications and inversions.
Comment: How to use LOEUF plugin in VEP?
by
Sd
• 0
Inside the loeuf.pm there is a link for the LOEUF scores that can be downloaded from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334197/b…
Comment: Visualise duplications and inversions
by
Medhat
9.8k
Maybe try samplot: https://github.com/ryanlayer/samplot
Comment: How can I identify genes with the MSTRG notation?
by
GenoMax
150k
More on MSTRG identifiers -- https://www.biostars.org/p/282817/#282832
Answer: Exon-level counts via featureCounts
by
GenoMax
150k
> I understand I need and exon-level count data Then you should use # Level of summarization -f Perform rea…
Comment: Picard : Bait_interval and Target_interval
by
GenoMax
150k
Are the intervals different in the two files or just that one file has the name (which is not essential for the tool). As noted in thread P…
Comment: Picard : Bait_interval and Target_interval
by
AIMAR
• 0
Thanks for the response. I've a targeted panel of genes. But if I have the bed.file I can obtain the target and bait file by using picard B…
Comment: Short reads to identify stuctural variants
by
GenoMax
150k
> Short reads aligned well within the inserted sequence but showed poor alignment in the flanking regions. If you have short-reads that c…
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