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120,839 results • Page
1 of 2417
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Votes
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0
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0
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43
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Understanding Re-use of Query Sequences in Long Read Alignments
Alignment
PacBio
pbmm2
Minimap2
6 hours ago by
Charles-Alexandre Roy
▴ 50
0
votes
2
replies
126
views
Discrepancy between BAM and vcf
discrepancy
vcf
DRAGEN
updated 11 hours ago by
Istvan Albert
102k • written 15 hours ago by
louis-gil
• 0
2
votes
5
replies
249
views
Question about `vg giraffe`
vg
updated 15 hours ago by
GenoMax
150k • written 1 day ago by
zhengluo
• 0
0
votes
1
reply
369
views
Difference in mapping rate when aligned with mapper.pl of miReep2 and bowtie1
small-RNASeq
miRNASeq
bowtie
miRDeep2
updated 16 hours ago by
Ram
45k • written 9 months ago by
MIKA
• 0
835
votes
169
replies
171k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 6 months ago by
Biostar
3.4k • written 8.3 years ago by
Istvan Albert
102k
1
vote
2
replies
175
views
How to align library of highly similar sequences
MPRA
variant
alignment
BWA
SNV
updated 20 hours ago by
ATpoint
87k • written 1 day ago by
rustyshackleford
• 0
0
votes
3
replies
1.4k
views
How to introduce normalized and scaled seurat data into monocle 3?
Monocle
scRNA-seq
Seurat
updated 20 hours ago by
Bastien Hervé
6.2k • written 22 months ago by
Sun
• 0
0
votes
0
replies
94
views
Reannotation of complete bacterial genome sequence
re-annotation
genome
whole
Automated
1 day ago by
csag6433
• 0
0
votes
2
replies
492
views
What are the best tools for quantifying allele-specific expression from bulk RNA-seq data these days?
ase
phASER
allele
gene
gatk
updated 1 day ago by
GenoMax
150k • written 1 day ago by
Paulo
• 0
3
votes
4
replies
3.1k
views
Lift over of GWAS summary stat file from Hg38 to Hg19
Hg38
linux
Liftover
GWAS
Hg19
updated 10 hours ago by
Mllepnos
• 0 • written 3.8 years ago by
AVA
▴ 40
8
votes
3
replies
218
views
How do I install Terminal on Windows?
Terminal
updated 1 day ago by
5heikki
11k • written 1 day ago by
phyms
• 0
1
vote
1
reply
126
views
Mapping reversion mutations
NGS
reversion
mutation
updated 1 day ago by
Ram
45k • written 1 day ago by
reddyraghuveer664
• 0
0
votes
0
replies
93
views
Issue with Fetching Population Allele Frequency in gnomAD GraphQL API
allelle
frequency
api
GraphQL
gnomad
1 day ago by
DareDevil
★ 4.4k
0
votes
1
reply
127
views
Attempts to demultiplex long reads from .pod5 result in unclassified reads
dorado
sequencing
Long-read
demultiplex
updated 1 day ago by
GenoMax
150k • written 1 day ago by
Placeholder@12654926
• 0
0
votes
1
reply
155
views
Issue with BSgenomeForge::forgeBSgenomeDataPkgFromNCBI
R
BSgenomeForge
BSgenome
NCBI
updated 1 day ago by
ATpoint
87k • written 1 day ago by
noodle
▴ 640
1
vote
1
reply
160
views
Batch effect or biological difference
Batch-effect
updated 1 day ago by
Ram
45k • written 2 days ago by
cynthier
• 0
3
votes
2
replies
199
views
Inconsistency between VCF and HGVS
VEP
1 day ago by
Senanu
▴ 30
0
votes
0
replies
109
views
VCFtools + easySFS snp # discordance
RADtags
vcftools
updated 2 days ago by
GenoMax
150k • written 2 days ago by
laurasachica7
• 0
0
votes
4
replies
3.1k
views
Generating Multiple Alignment Format file (Maf)
maf
multiple-alignment
mafft
updated 2 days ago by
Ram
45k • written 8.3 years ago by
roz_safavi
• 0
3
votes
6
replies
317
views
STAR: Paired alignment gets ~18% unmapped (too short), but single reads get >90% mapping
paired-end
star
rnaseq
1 day ago by
Davor
• 0
1
vote
0
replies
181
views
Job:
bioinformatician position, Ohio State University, Columbus, USA
bioinformatician
updated 2 days ago by
GenoMax
150k • written 2 days ago by
ilaria.palmisano
▴ 10
3
votes
4
replies
255
views
Coverage drop at assembly ends
bowtie2
bw-mem
alignment
minimap2
updated 2 days ago by
GenoMax
150k • written 2 days ago by
alenew.am
▴ 10
1
vote
4
replies
331
views
How to identify additional SNPs on EPICv2
SNPs
methylation
EPIC
2 days ago by
Basti
★ 2.0k
0
votes
1
reply
168
views
jcvi:ValueError: A total of 0 anchor was found. Aborted.
jcvi
Collinearity
updated 2 days ago by
Ram
45k • written 2 days ago by
JieQY
• 0
0
votes
5
replies
361
views
SNPcheck alternative
SNPCheck
updated 2 days ago by
GenoMax
150k • written 3 days ago by
KirGen
▴ 30
0
votes
0
replies
127
views
Comparing HMMER vs. HHrepID for LRR Repeat Detection and Boundary Consistency
hmmer
repeats
2 days ago by
jllPons
• 0
0
votes
0
replies
161
views
circos plot help
visualization
SNV
genomics
circos
updated 1 day ago by
Ram
45k • written 3 days ago by
Genomancer
• 0
2
votes
3
replies
254
views
DAVID List Upload/Gene Conversion Tool
DAVID
updated 2 days ago by
Ram
45k • written 3 days ago by
b5708137
• 0
0
votes
8
replies
2.9k
views
6 follow
CIBERSORTx error: 'x' must be an array of at least two dimensions
matrix
CIBERSORTx
signature
updated 3 days ago by
GenoMax
150k • written 2.7 years ago by
Jie
• 0
1
vote
2
replies
528
views
Can I combine segment results from Sequenza and cnvkit?
CNV
sequenza
cnvkit
3 days ago by
Ram
45k
1
vote
6
replies
370
views
Shotgun dataset with polyG, low qualiity
shotgun-data
updated 1 day ago by
andres.firrincieli
3.9k • written 3 days ago by
shevch2009
• 0
0
votes
2
replies
229
views
MAGeCK: Doing two sided test on gene level?
MAGeCK
3 days ago by
gernophil
▴ 120
0
votes
5
replies
329
views
Is TETranscripts useful for repetitive sequences analysis?
TEtranscripts
updated 2 days ago by
Michael
55k • written 3 days ago by
frarodmar17
• 0
2
votes
5
replies
318
views
SlingShot and Subsetting data
scrna
slingshot
trajectory
updated 1 day ago by
jared.andrews07
★ 18k • written 3 days ago by
carolofharvest
▴ 50
0
votes
1
reply
326
views
Bulk searching UniProt
uniprot
updated 3 days ago by
Elisabeth Gasteiger
★ 2.4k • written 7 days ago by
timothy.kirkwood
▴ 140
4
votes
6
replies
337
views
Problem with Accession Number Changes in UniProt FASTA Sequences
FASTA
Accession
Uniprot
IdMapping
2 days ago by
bioinfo_enthusiast
• 0
1
vote
2
replies
374
views
PPI Network Analysis of Virulence Genes in Morganella morganii Using STRING Database
PPI
STRING
3 days ago by
bioinfo_enthusiast
• 0
15
votes
12
replies
707
views
7 follow
Forum:
Reproducibility Crisis
R-Bioinformatics
updated 1 day ago by
i.sudbery
21k • written 4 days ago by
ParastooA
▴ 20
6
votes
13
replies
7.4k
views
7 follow
Getting sample information from GEO
GEO
NCBI
updated 3 days ago by
DareDevil
★ 4.4k • written 7.9 years ago by
Tom_L
▴ 360
0
votes
3
replies
252
views
How to Close a Phage Genome? Issues with PhageTerm and CheckV Completeness
phage
genome
assembly
PhageTerm
updated 3 days ago by
GenoMax
150k • written 3 days ago by
mail2steff
▴ 70
2
votes
2
replies
250
views
RepBase30.02.fasta.tar.gz
RepBase30.02.fasta.tar.gz
offtopic
updated 3 days ago by
Michael
55k • written 4 days ago by
jupitoy
• 0
1
vote
5
replies
1.2k
views
CIBERSORTx error (error in rep(2, size * length(cells) -1)) : invalid 'times' argument
deconvolution
RNA-seq
CIBERSORTx
CIBERSORT
updated 6 weeks ago by
zhbyrd
• 0 • written 9 months ago by
Mr. George
▴ 10
1
vote
2
replies
287
views
Differences in DEG results using RSEM with and without the --strandness reverse
rsem
DEG
STAR
transcriptomic
updated 3 days ago by
LauferVA
4.6k • written 4 days ago by
Emy Alade
• 0
3
votes
5
replies
430
views
Absolute vs. differential gene expression - which analysis is more informative?
Differential-gene-expression
Gene-expression
RNA-Seq
updated 3 days ago by
LauferVA
4.6k • written 4 days ago by
bioinfo1990
▴ 10
2
votes
3
replies
369
views
making psam file for plink2
plink2
3 days ago by
dec986
▴ 380
4
votes
4
replies
339
views
gtf file for canFam2 genome version
gtffile
3 days ago by
1769mkc
★ 1.2k
2
votes
2
replies
393
views
Bioinformatics
StringDB
updated 1 day ago by
Ram
45k • written 4 days ago by
ahmad79rezahay
• 0
0
votes
0
replies
187
views
News:
Epigenomics Data Analysis
Epigenomics
RNA-seq
ATAC-seq
Chip-seq
HI-C
4 days ago by
Physalia-courses
★ 2.6k
4
votes
8
replies
598
views
does any licenses needed to make experiments?
offtopic
updated 4 days ago by
Dunois
★ 2.9k • written 5 days ago by
suleyman
• 0
6
votes
5
replies
376
views
Strange overrepresented sequence
fastQC
adapter
rnaseq
4 days ago by
georomano
• 0
120,839 results • Page
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Comment: SlingShot and Subsetting data
Comment: SlingShot and Subsetting data
Reproducibility Crisis
Comment: Shotgun dataset with polyG, low qualiity
Comment: Batch effect or biological difference
Comment: Inconsistency between VCF and HGVS
Answer: Lift over of GWAS summary stat file from Hg38 to Hg19
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Comment: Associating VDJ clonotyping data with scRNA-seq in Seurat
by
garcesj
▴ 50
It's a great workaround. My only question is about just removing the duplicates (`tcr <- tcr[!duplicated(tcr$barcode), ]`). Aren't we losin…
Comment: Discrepancy between BAM and vcf
by
Istvan Albert
102k
I will also say that if you take any variant caller and then look at the corresponding BAM file, you'll find many minor inconsistencies - i…
Comment: Lift over of GWAS summary stat file from Hg38 to Hg19
by
Mllepnos
• 0
Thanks a lot for the recommendation to use bcftools +munge and +liftover instead of UCSC liftOver — I’ll definitely try that!
Comment: Discrepancy between BAM and vcf
by
GenoMax
150k
You could write to Illumina tech support and ask for resources that explain the default parameters for SNP calling/filtering in DRAGEN pipe…
Comment: Error gmx_mmpbsa: LP pseudo-atom is not supported
by
namck
• 0
Hi @bhavya2269, did you find any solution to this? I'm getting a similar error.
Comment: Question about `vg giraffe`
by
GenoMax
150k
You recent posts appear to be unrelated to the original question. If that is correct please post new questions in new threads. Posting unre…
Comment: Seeking Guidance on Selecting Control ChIP Sequences for nf-core/chipseq Pipelin
by
mark.ziemann
★ 2.0k
These are not really technical replicates if they simply re-sequenced the same library three times. Resequencing multiplexed libraries on d…
Comment: Question about `vg giraffe`
by
zhengluo
• 0
``` vg construct --reference ref.fa --vcf test.vcf.gz --alt-paths-plain --handle-sv --flat-alts --progress -t 100 > test.vg vg index --xg…
Answer: Difference in mapping rate when aligned with mapper.pl of miReep2 and bowtie1
by
wessel.willemsen
• 0
I had the same problem as you but i solved it by allowing the reads to map to more targets. By default mapper.pl does not allow a read to m…
Comment: Question about `vg giraffe`
by
zhengluo
• 0
Given a sample (sample1) with 100 insertions relative to the reference genome, can I identify which reads in the BAM file aligned to these …
Comment: Question about `vg giraffe`
by
zhengluo
• 0
I directly constructed the graph using VCF files and reference genome sequences through `vg construct`, but after converting it to GFA form…
Comment: How to introduce normalized and scaled seurat data into monocle 3?
by
Bastien Hervé
6.2k
For OP's question, my guess is that they got NA's because `ScaleData` is only called on the `VariableFeatures` found the step before. Try …
Answer: How to align library of highly similar sequences
by
ATpoint
87k
You need to deviate from the defaults of the aligner to enforce that only perfect matches are counted. Basically, and I use bowtie2 for pur…
Answer: Question about `vg giraffe`
by
Jouni Sirén
▴ 610
The easiest way is to align the reads to the graph in GAM or GAF format. Then you select sample `sample` as the reference using `vg gbwt`: …
Answer: How to introduce normalized and scaled seurat data into monocle 3?
by
Reza
• 0
I have a same question it seems that when we use the preprocess_cds() function, it does not save the normalized data directly in a separate…
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