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120,796 results • Page
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Votes
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7
votes
6
replies
207
views
Heatmap problem in R
R
Heatmap
1 hour ago by
ParastooA
• 0
2
votes
2
replies
55
views
Bacterial VCF file annotation using snpEff error
SnpEff
54 minutes ago by
1769mkc
★ 1.2k
0
votes
1
reply
41
views
Coverage depth using samtools
samtools
ngs
depth
sequencing
coverage
updated 1 hour ago by
Pierre Lindenbaum
165k • written 4 hours ago by
slzr_
• 0
0
votes
2
replies
89
views
MarkDuplicates RNASeq: A few samples look weird. What could be the cause?
qc
markduplicates
picard
rnaseq
updated 4 hours ago by
GenoMax
150k • written 12 hours ago by
Davor
• 0
0
votes
1
reply
60
views
Data Integrity (NCBI SRA and TCGA)
clinic
metadata
updated 4 hours ago by
GenoMax
150k • written 7 hours ago by
umarfaruksahin
• 0
0
votes
0
replies
162
views
concatenating 2 differently-imputed VCF files then filtering by imputation score to keep the higher imputation score imputed vaiant from duplicate v…
Imputation
filtration
score
duplicate
variants
10 hours ago by
SalmaElShafie
• 0
835
votes
169
replies
171k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 6 months ago by
Biostar
3.4k • written 8.3 years ago by
Istvan Albert
102k
0
votes
0
replies
44
views
loss of coverage using gargammel
gargammel.pl
ART
AdapterRemoval
11 hours ago by
anna.utili
• 0
0
votes
1
reply
67
views
What is the stance on optical duplicates in RNASeq?
duplicates
rnaseq
optical-duplicates
updated 4 hours ago by
GenoMax
150k • written 12 hours ago by
Davor
• 0
0
votes
3
replies
165
views
How to rank genes for GSEA using edgeR-LRT results ?
DEG
edgeR
updated 17 hours ago by
Gordon Smyth
★ 7.8k • written 1 day ago by
Picasa
▴ 650
0
votes
3
replies
230
views
Using bulk RNA-seq DE results to perform PCA in single cell RNA-seq
Single-cell
scRNAseq
updated 18 hours ago by
swbarnes2
14k • written 2 days ago by
Oli
• 0
3
votes
1
reply
108
views
Access to dbSnp137 (GRCh37)
dbSnp137
GRCh38
dbSnp
updated 23 hours ago by
Marcellie87
▴ 10 • written 1 day ago by
Steven
▴ 50
0
votes
0
replies
59
views
How to convert RAP-DB/MSU IDS to RefSeq/Entrez Gene IDS?
IRGSP
Entrez
RAP-DB
RefSeq
1 day ago by
ChanderKant (CK) Chaudhary
▴ 10
4
votes
4
replies
1.6k
views
Annotating single cell data automatically
single-cell
updated 1 day ago by
S.Ghazala
▴ 60 • written 11 months ago by
Gerard
▴ 10
0
votes
0
replies
78
views
Bulk searching UniProt
uniprot
1 day ago by
timothy.kirkwood
▴ 140
3
votes
0
replies
109
views
News:
Join the online/distributed nf-core Hackathon next week!
nf-core
nextflow
container
workflows
bioinformatics
1 day ago by
Matthias Zepper
5.1k
0
votes
1
reply
104
views
Non variants sites of the genome (gVCF file)
non
genome
variants
sites
gVCF
updated 14 hours ago by
LauferVA
4.6k • written 1 day ago by
heureuse
▴ 10
1
vote
1
reply
117
views
Choosing the "right" sequence as an input control
input
control
chipseq
updated 1 day ago by
ATpoint
87k • written 1 day ago by
mark.pekarsky
▴ 20
1
vote
2
replies
127
views
Unable to parse config file
nextflow
nf-core
Pulled
form
github
updated 12 hours ago by
Phil Ewels
★ 1.4k • written 1 day ago by
JieQY
• 0
0
votes
0
replies
78
views
Seeking Guidance on Selecting Control ChIP Sequences for nf-core/chipseq Pipeline
SRR
nf-core
chipseq
1 day ago by
mark.pekarsky
▴ 20
0
votes
0
replies
73
views
Problem with GSVA output
GSVA
RNAseq
1 day ago by
a.stef.44
▴ 10
0
votes
2
replies
138
views
Using UMI-tools on Smart-seq3 RNA-Seq data
rna-seq
UMI
UMI-tools
smart-seq3
updated 1 day ago by
Ram
45k • written 1 day ago by
Agastya
▴ 10
1
vote
5
replies
277
views
Remove site with only missing data
Fasta
updated 1 day ago by
Pierre Lindenbaum
165k • written 2 days ago by
Leane
• 0
0
votes
3
replies
187
views
Problems with Seaborn plots in Python
python
sns
plot
updated 1 day ago by
Wayne
★ 2.1k • written 1 day ago by
egascon
▴ 60
4
votes
4
replies
320
views
Downloading full list of Homo sapiens genes
Genes
1 day ago by
nigussie.amu
• 0
0
votes
0
replies
121
views
How to normalise Nanopore mRNA sequencing data between two cell lines
Nanopore
RNAseq
Normalization
2 days ago by
Mo
▴ 50
0
votes
0
replies
262
views
Can I combine segment results from Sequenza and cnvkit?
CNV
sequenza
cnvkit
2 days ago by
Ram
45k
2
votes
5
replies
4.1k
views
Python library for parsing bcftools stats file
bcftools
updated 2 days ago by
Pierre Lindenbaum
165k • written 7.6 years ago by
William
★ 5.3k
0
votes
0
replies
113
views
Manta SV (specially Translocation)
manta
Structural-Variant
updated 1 day ago by
Ram
45k • written 2 days ago by
Nai
▴ 50
0
votes
3
replies
394
views
Cytoscape COLOUR EDGES
Reulatory
Cytoscape
Networks
updated 2 days ago by
Scooter
▴ 280 • written 10 days ago by
SKY
▴ 60
2
votes
2
replies
1.1k
views
How to perform PPI network analysis in STRING for newly sequenced bacterial genome ?
Proteomics
cytoscape
network
STRING
updated 2 days ago by
Scooter
▴ 280 • written 3.8 years ago by
Kumar
▴ 120
0
votes
0
replies
117
views
How to perform burden test using plink /seq
plink-seq
Burden-Test
updated 1 day ago by
Ram
45k • written 2 days ago by
aiswaryabioinfo
▴ 30
0
votes
3
replies
186
views
Higher number of reads mapped to transcriptome vs genome in star mapping
riboseq
star
bam
transcriptome
alignment
updated 2 days ago by
i.sudbery
21k • written 2 days ago by
paguirreazorin
• 0
1
vote
4
replies
318
views
Visualisation of read depth
coverage
read-depth
variant-calling
updated 1 day ago by
Ram
45k • written 2 days ago by
AIMAR
• 0
7
votes
5
replies
692
views
Tool:
AliNe (Alignment in Nextflow)
nextflow
alignment
2 days ago by
Juke34
9.2k
0
votes
0
replies
127
views
construct PKN
REGULATORY
PKN
MOUSE
NETWORKS
GENE
2 days ago by
rahma.khoualdia
• 0
2
votes
4
replies
282
views
Tool for automatic immune cell annotation in MOUSE?
immune
single-cell
annotation
updated 1 day ago by
theHumanBorch
▴ 260 • written 2 days ago by
txema.heredia
▴ 210
4
votes
12
replies
7.0k
views
12 follow
How to draw venn diagram for two CNV lists?
venn
updated 2 days ago by
5heikki
11k • written 10.3 years ago by
lyz10302012
▴ 480
0
votes
2
replies
192
views
merging diploid and tetraploid vcfs
bcftools
diploid
tetraploid
2 days ago by
analyst
▴ 60
0
votes
1
reply
163
views
TMM normalized CPM
TPM
CPM
TMM
updated 1 day ago by
ATpoint
87k • written 2 days ago by
maryak
▴ 20
0
votes
0
replies
119
views
Normlizatiom in DiffBind
DiffBind
CHIP-Seq
2 days ago by
Siqi
• 0
0
votes
1
reply
156
views
Obtain genome coordinates for a DNA sequence using ENSEMBL API
Ensembl
API
updated 2 days ago by
GenoMax
150k • written 2 days ago by
Ishan
• 0
0
votes
0
replies
132
views
Forum:
Decoding Corvid Intelligence: Genomics, AI & the Genetics of Cognition
neurogenetics
comparative-genomics
bioinformatics
genomics
machine-learning
updated 2 days ago by
Ram
45k • written 2 days ago by
EvoGenExplorer
• 0
2
votes
4
replies
311
views
GBWT Construction Error: "Sources X and Y both have node Z" When Building GBZ from GFA
vg
18 hours ago by
Sauers
▴ 20
8
votes
16
replies
608
views
6 follow
prokka with too many "hypothetical proteins" and genome comparison
comparison
genome
galaxy
prokka
2 days ago by
avinci1
▴ 20
0
votes
1
reply
209
views
Split paired ended hWGS FASTq files to simulate difference sequence coverage
whole-genome-sequencing
wgs
FASTQ
coverage
updated 3 days ago by
shelkmike
★ 1.5k • written 3 days ago by
DJBill
▴ 20
0
votes
8
replies
424
views
OMA use case
oma
omastandalone
1 day ago by
nmalexan
• 0
0
votes
0
replies
146
views
News:
Machine Learning for Longitudinal Data with Python – Online Course (6-9 May)
MachineLearning
LongitudinalData
DeepLearning
Omics
3 days ago by
Physalia-courses
★ 2.6k
1
vote
2
replies
344
views
Recommended workflow to pre-process and demultiplex scRNAseq data based on the HTOs and ADTs
ADT
HTO
demultiplexing
scRNAseq
updated 3 days ago by
GenoMax
150k • written 3 days ago by
mete.han.celebi
• 0
0
votes
3
replies
231
views
bwa-mem keeps failing to locate the index files
bwa
bwa-mem
updated 3 days ago by
ATpoint
87k • written 3 days ago by
pairedttest
▴ 30
120,796 results • Page
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Recent Votes
Comment: Bacterial VCF file annotation using snpEff error
Comment: Bacterial VCF file annotation using snpEff error
Answer: Heatmap problem in R
Answer: Heatmap problem in R
Comment: Heatmap problem in R
Comment: nanopore variant calling
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Recent Replies
Comment: Bacterial VCF file annotation using snpEff error
by
1769mkc
★ 1.2k
okay I will explore this and update...
Comment: Heatmap problem in R
by
ParastooA
• 0
Just another question If I want to perferm a data cleaning on Matrix_ series what elements should I consider for doing so?
Comment: Heatmap problem in R
by
ParastooA
• 0
Thanks everyone. yesturday I was a little overwhelmed and now I see your point about sharing the code.
Comment: Coverage depth using samtools
by
Pierre Lindenbaum
165k
yes but the option -b is missing a file if you want to compute all bases overlapping a bed file. https://www.htslib.org/doc/samtools-depth…
Comment: Bacterial VCF file annotation using snpEff error
by
Pierre Lindenbaum
165k
in https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_001884025.1/ and if you look at the files under the FTP tab, you'll see many differen…
Comment: What is the stance on optical duplicates in RNASeq?
by
GenoMax
150k
> but optical ones are a technical artifact Generally these are applicable only if your data was run on patterned flowcells (which may be …
Comment: Data Integrity (NCBI SRA and TCGA)
by
GenoMax
150k
> I have sequencing data from the NCBI SRAdatabase. As in raw sequence data from dbGaP and not just counts? So you did get permission to …
Comment: MarkDuplicates RNASeq: A few samples look weird. What could be the cause?
by
GenoMax
150k
Is this different data than what we were discussing in last two questions? Simple explanation may be that the quality of initial RNA was po…
Answer: MarkDuplicates RNASeq: A few samples look weird. What could be the cause?
by
ATpoint
87k
What do you want to do? Differential analysis? If so, ignore all these lowlevel metrics and see how samples compare in a PCA and how dow…
Comment: Unable to parse config file
by
Phil Ewels
★ 1.4k
Agreed, I also just tested it (with the following to try to mimic your setup exactly): ```bash NXF_VER=24.10.5 nextflow run nf-core/genome…
Comment: Non variants sites of the genome (gVCF file)
by
LauferVA
4.6k
Hi Heureuse ... do you *have* to use a gVCF? Or could you use another format?
Answer: How to rank genes for GSEA using edgeR-LRT results ?
by
Gordon Smyth
★ 7.8k
The best choice would be use edgeR's built-in GSEA functionality provided by `camera` and `cameraPR`. These functions adjust for inter-gene…
Comment: How to rank genes for GSEA using edgeR-LRT results ?
by
Gordon Smyth
★ 7.8k
Don't necessarily need to run `topTags`. Even with the `glmLRT` output, `lrt` say, the signed LRT statistic ``` z <- sign(lrt$table$logFC) …
Comment: What are the advantages of using the T2T as a reference vs GRCh38 today?
by
Gordon Smyth
★ 7.8k
The T2T genome is derived from cell lines of European origin. On digging deeper, we found that the GRCh38 sequences for immunoglobin genes …
Comment: Use limma for correlation analysis of RNA-seqdata and continous trait
by
Gordon Smyth
★ 7.8k
The coefficient (logFC) is the log2 expression change for each unit change in the continuous covariate. The interpretation follows the usu…
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