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121,144 results • Page
1 of 2423
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Tool for Estimation of Purity and Ploidy in Precancerous Lesions
CNV
purity
genome
ploidy
6 hours ago by
ting
• 0
8
votes
8
replies
492
views
6 follow
Nextflow: split a FASTA file into 5 parts to enable parallel processing
nextflow
workflow
pipeline
9 hours ago by
neng
▴ 30
837
votes
170
replies
176k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 12 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
0
votes
0
replies
55
views
What to choose as background in statistic test genetics
test
expression
gene
statistic
10 hours ago by
adigershon8897
• 0
0
votes
4
replies
300
views
differential exon usage from Salmon outputs
edgeR
diffSpliceDGE
DEU
salmon
updated 10 hours ago by
Gordon Smyth
★ 7.9k • written 3 days ago by
n_navy
• 0
0
votes
0
replies
66
views
Multi-SNP Behavioral and Frequency Profile Inquiry: TPH2, GRM2, COMT, DRD2, HTR1A (Rare Interaction)
population-genetics
genetic-variation
epistasis
snp
gnomad
13 hours ago by
SonicSoal
• 0
0
votes
0
replies
75
views
Rare variants association analysis (using WGS)
variant
GWAS
rare
WGS
17 hours ago by
SeoG
• 0
0
votes
0
replies
87
views
Selecting clinical rows for repeated case IDs in TCGA-BRCA data
Clinical-Data
Breast-Cancer
TCGA
updated 18 hours ago by
Ram
45k • written 1 day ago by
BhagyashreeWaghale
• 0
0
votes
0
replies
84
views
Pooling Replicates in Omics Analysis: Trade-off Between Cost and Data Robustness?
Replicates
Omics
Pooling
1 day ago by
sardius
• 0
0
votes
0
replies
88
views
Convert AnnData to Seurat (spatial transcriptomics, visium HD)
seurat
segmentation
anndata
visiumhd
updated 1 day ago by
GenoMax
151k • written 1 day ago by
npont
• 0
0
votes
0
replies
93
views
News:
Online course -Reproducibility in Bioinformatics - July 7–9
Singularity
Git
Docker
Snakemake
Nextflow
1 day ago by
Physalia-courses
★ 2.6k
1
vote
5
replies
318
views
RNA-Sequencing Analysis when control samples paired-end and tumour samples single-end
ENA
rna-sequencing
1 day ago by
h
• 0
0
votes
3
replies
214
views
STAR aligner - how is quality and correctness tested across builds/releases?
STAR
updated 1 day ago by
GenoMax
151k • written 1 day ago by
Arthur
• 0
1
vote
2
replies
207
views
Pathway enrichment analysis on time-series data by filtering out age comparisons
pea
go
rnaseq
time-series
1 day ago by
fullycratered
• 0
4
votes
4
replies
254
views
building snpeff database for plant
plant
1 day ago by
analyst
▴ 60
1
vote
1
reply
142
views
How to find motif in specific region
motif
chip-seq
updated 1 day ago by
Alex Reynolds
36k • written 1 day ago by
zhang616123
• 0
0
votes
0
replies
125
views
How to interpret perplexing qcovhsp result in blastx
blastx
qcovhsp
blastn
1 day ago by
samuel.himes
• 0
0
votes
0
replies
106
views
Representation of GT:DS INFO tag for a missing marker in a VCF file
VCF
bcftools
2 days ago by
Mwangana
• 0
0
votes
0
replies
114
views
Best Practices for Integrating RNA-seq Time Series from Multiple Studies with Varying Protocols
differential-expression
meta-analysis
integration
RNA-seq
time-series
2 days ago by
enee
▴ 20
10
votes
9
replies
4.8k
views
bcftools query add header
bcftools
updated 2 days ago by
GenoMax
151k • written 3.1 years ago by
User000
▴ 750
0
votes
2
replies
250
views
Alternative to PASApipeline
transcriptomics
annotation
updated 1 day ago by
colindaven
7.4k • written 3 days ago by
lagartija
▴ 160
0
votes
0
replies
124
views
How to Get a Genetic Map, Given the Physical Position and Genotype Data of Markers
genetic-map
updated 2 days ago by
Ram
45k • written 2 days ago by
Daniyal
• 0
1
vote
7
replies
2.4k
views
vcftools --weir-fst-pop returns -nan
fst
vcftools
updated 2 days ago by
Michael
55k • written 2.4 years ago by
elizabeth
• 0
0
votes
2
replies
186
views
HISAT2 with Cleaned Data
HISAT2
RNASeq
50 minutes ago by
Gordonz9494
• 0
2
votes
1
reply
197
views
Definitions for "differential expression" and "differential abundance" in RNA-seq?
rna-seq
deseq
abundance
differential-expression
updated 2 days ago by
Ram
45k • written 2 days ago by
tw_140
• 0
0
votes
5
replies
330
views
Efficiently find regions in bed file that are within start and end regions of another bed file.
intersect
bedtools
updated 2 days ago by
Alex Reynolds
36k • written 3 days ago by
rxs1018
• 0
2
votes
4
replies
264
views
16S Libraries With Non-Purified Short Fragments
Sequencing
16S
Libraries
Magnetic
Beads
2 days ago by
alenew.am
▴ 10
0
votes
1
reply
201
views
How to map polyA tails to barcodes in paired end reads for single cell RNA-seq
RNA-seq
cell
polyA
single
updated 3 days ago by
GenoMax
151k • written 3 days ago by
Ana
▴ 10
7
votes
6
replies
4.9k
views
Questions related to ChromHMM+using MACS2 peak calling output bed file as input for ChromHMM?
macs2
ChIP-Seq
ChromHMM
MACS2
CHROMHMM
updated 3 days ago by
luminea
▴ 10 • written 5.9 years ago by
m.sadman.sakib
▴ 120
4
votes
5
replies
448
views
Editing and Adding to a GTF file
Annotation
Gene-ID
GTF
updated 2 days ago by
Ram
45k • written 4 days ago by
Keem Uarren Eiryll
▴ 10
4
votes
2
replies
214
views
Averaging multiple IgG BAM files for use as a control in ChromHMM
ChromHMM
bam
averag
updated 3 days ago by
ATpoint
88k • written 3 days ago by
luminea
▴ 10
3
votes
3
replies
431
views
Deduplication rate of shotgun metagenomes using fastqc
metagenome
fastqc
shotgun
deduplication
updated 3 days ago by
colindaven
7.4k • written 8 days ago by
vikasmh111
• 0
0
votes
0
replies
147
views
News:
online course: Introduction to Genome Annotation
Gene-Prediction
Genome-Annotation
Gene-Models
3 days ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
978
views
ValueError: Found array with 0 sample(s) (shape=(0, 138)) while a minimum of 1 is required. In metawrap - concoct binning module
concoct
metawrap
module
3 days ago by
shevch2009
• 0
2
votes
7
replies
417
views
Circular heatmap using circos.heatmap in R
R
circos
heatmap
circularheatmap
updated 2 days ago by
GenoMax
151k • written 3 days ago by
Yukta
• 0
0
votes
0
replies
177
views
Zebrafish phenotype ontology enrichment analyses
ontology
zebrafish
3 days ago by
Li
• 0
0
votes
2
replies
263
views
Sequence Duplication for Single-End 3RAD Data
duplication
reduced-representation
3 days ago by
Sarah
• 0
0
votes
0
replies
178
views
Cytoscape KEGG plots reverting to original layout after changing style or column
kegg
cytoscape
4 days ago by
Adrian
• 0
1
vote
6
replies
1.1k
views
6 follow
Not retrieving Mitochondrial Genes in Single Cell analysis
single-cell
updated 3 days ago by
Ram
45k • written 10 months ago by
guioliveirareis
▴ 10
0
votes
0
replies
177
views
News:
Ancient Metagenomics course - online, May 27-29
ancient-metagenomics
aDNA
aMeta
4 days ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
222
views
Issues running scAPAtrap
scAPAtrap
polyA
scRNAseq
updated 4 days ago by
GenoMax
151k • written 4 days ago by
Ana
▴ 10
5
votes
3
replies
334
views
What are BED files?
BED
updated 3 days ago by
Alex Reynolds
36k • written 4 days ago by
Besoa
• 0
3
votes
8
replies
513
views
ggplot make boxplots more wide
ggplot2
r
4 days ago by
Nona
▴ 90
2
votes
1
reply
278
views
Minimum number of samples for multi-omics data integration
multi-omics
updated 4 days ago by
Ram
45k • written 4 days ago by
Tundup
▴ 10
1
vote
0
replies
216
views
News:
Workshop: Virtual Genome Annotation, May 27–29
GenomeAnnotation
workshop
UConn
4 days ago by
zsc25001
▴ 10
1
vote
1
reply
264
views
Assigning a string variable on Visual Studio using python
python
visual-studio
updated 4 days ago by
Ram
45k • written 5 days ago by
arthur_alexander83
• 0
4
votes
4
replies
398
views
Tool:
Gene Exons Painter
Genomics
Visualisation
Exons
Transcripts
Isoforms
updated 4 days ago by
benformatics
4.1k • written 5 days ago by
Rafal
▴ 70
0
votes
0
replies
214
views
News:
Course online:Functional programming in R, May 27-30
R
programming
functional
5 days ago by
soledad.esteban
• 0
3
votes
1
reply
299
views
Herald:
The Biostar Herald for Monday, May 05, 2025
herald
updated 5 days ago by
cmdcolin
★ 4.2k • written 5 days ago by
Biostar
3.5k
0
votes
0
replies
198
views
How to generate .AED files from .CEL files using Linux (Affymetrix CytoScan HT-CMA 96F)
HT-CMA-96
analysis
data
affymetrix
microarray
5 days ago by
genetixs
▴ 20
121,144 results • Page
1 of 2423
Recent Votes
Answer: Lift over of GWAS summary stat file from Hg38 to Hg19
Answer: Nextflow: split a FASTA file into 5 parts to enable parallel processing
Answer: Definitions for "differential expression" and "differential abundance" in RNA-se
Comment: Nextflow: split a FASTA file into 5 parts to enable parallel processing
Answer: How to find motif in specific region
Mappability file for hg38
C: Mappability file for hg38
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151k
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chuanj8848
• 0
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★ 2.6k
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Comment: HISAT2 with Cleaned Data
by
Gordonz9494
• 0
Hi, Thank you for your comment. Regarding your question about the "counts are too low," I’m working with the cleaned data provided by th…
Answer: Nextflow: split a FASTA file into 5 parts to enable parallel processing
by
neng
▴ 30
Ok, I finally find the solution. I would like to share with you guys: workflow { // Step 7: split SplitFasta(params.fasta)…
Answer: differential exon usage from Salmon outputs
by
Gordon Smyth
★ 7.9k
If you want to detect differential splicing using edgeR with Salmon output, then you should do DTU instead of DEU, see: Baldoni PL#, Chen …
Comment: Nextflow: split a FASTA file into 5 parts to enable parallel processing
by
neng
▴ 30
Yeah, it's a good solution I've tried. The only problem is that the SplitFasta() process has to be finished before it starts, otherwise it …
Comment: differential exon usage from Salmon outputs
by
dsull
★ 7.5k
Providing each exon as an individual transcript will throw off pseudoalignment-type algorithms which assume that each read originated from …
Comment: Create local Blast database alias with blastdb_aliastool fails
by
GenoMax
151k
Can you set the following variable and see if that helps. BLASTDB=f:/BlastDB/nr/nr export BLASTDB
Comment: building snpeff database for plant
by
analyst
▴ 60
Is it safe to add -noCheckCds -noCheckProtein in the command or will it skip important information in the resultant database: java -Xm…
Comment: Create local Blast database alias with blastdb_aliastool fails
by
etienne
• 0
Thank you. It is working now with: blastdbcmd -db f:/BlastDB/nr/nr -taxids 1988 -out f:/BlastDB/extract/acti3.fa But it displays thi…
Comment: Pathway enrichment analysis on time-series data by filtering out age comparisons
by
fullycratered
• 0
Ah that makes sense, I hadn't done the DE analysis myself in this case. I will look at doing that, thank you!
Comment: building snpeff database for plant
by
lieven.sterck
15k
I had a quick look and though it is feasible to change files (and specifically feature names) such that the files correspond to each other …
Comment: Nextflow: split a FASTA file into 5 parts to enable parallel processing
by
neng
▴ 30
what I expected: [part_001, /path/to/split/part_001.fasta] [part_002, /path/to/split/part_002.fasta] ... actually data stream…
Comment: STAR aligner - how is quality and correctness tested across builds/releases?
by
GenoMax
151k
> Coming from a software engineering background Curious as to how many people were part of your team in (I assume) commercial software dev…
Comment: Nextflow: split a FASTA file into 5 parts to enable parallel processing
by
Pierre Lindenbaum
166k
I don't understand how your splitfasta works, the output should collect the files under "split". It should be something like: ``` process …
Comment: STAR aligner - how is quality and correctness tested across builds/releases?
by
Arthur
• 0
Fair question - I single out STAR as I believe it's a widely used tool, but I am curious across the board of these 'widely used bioinformat…
Comment: RNA-Sequencing Analysis when control samples paired-end and tumour samples singl
by
h
• 0
Thanks for both your help I will do that!
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