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120,813 results • Page
1 of 2417
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Votes
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6
votes
13
replies
7.2k
views
7 follow
Getting sample information from GEO
GEO
NCBI
updated just now by
DareDevil
★ 4.4k • written 7.8 years ago by
Tom_L
▴ 360
0
votes
0
replies
7
views
How to Close a Phage Genome? Issues with PhageTerm and CheckV Completeness
phage
genome
assembly
PhageTerm
7 minutes ago by
mail2steff
▴ 70
0
votes
0
replies
16
views
How to identify additional SNPs on EPICv2
SNPs
methylation
EPIC
1 hour ago by
Basti
★ 2.0k
1
vote
1
reply
65
views
RepBase30.02.fasta.tar.gz
RepBase30.02.fasta.tar.gz
updated 3 hours ago by
Mensur Dlakic
★ 29k • written 7 hours ago by
jupitoy
• 0
1
vote
5
replies
1.0k
views
CIBERSORTx error (error in rep(2, size * length(cells) -1)) : invalid 'times' argument
deconvolution
RNA-seq
CIBERSORTx
CIBERSORT
updated 7 hours ago by
Andrew
• 0 • written 9 months ago by
Mr. George
▴ 10
835
votes
169
replies
171k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 6 months ago by
Biostar
3.4k • written 8.3 years ago by
Istvan Albert
102k
1
vote
2
replies
135
views
Differences in DEG results using RSEM with and without the --strandness reverse
rsem
DEG
STAR
transcriptomic
updated 7 hours ago by
LauferVA
4.6k • written 23 hours ago by
Emy Alade
• 0
2
votes
2
replies
144
views
Absolute vs. differential gene expression - which analysis is more informative?
Differential-gene-expression
Gene-expression
RNA-Seq
updated 8 hours ago by
LauferVA
4.6k • written 18 hours ago by
bioinfo1990
▴ 10
1
vote
2
replies
104
views
making psam file for plink2
plink2
updated 11 hours ago by
chrchang523
11k • written 12 hours ago by
dec986
▴ 380
3
votes
4
replies
163
views
gtf file for canFam2 genome version
gtffile
4 hours ago by
1769mkc
★ 1.2k
2
votes
2
replies
223
views
Bioinformatics
DB
String
updated 15 hours ago by
Mensur Dlakic
★ 29k • written 1 day ago by
ahmad79rezahay
• 0
6
votes
4
replies
150
views
Forum:
Reproducibility Crisis
R-Bioinformatics
updated 1 minute ago by
Bastien Hervé
6.1k • written 16 hours ago by
ParastooA
▴ 10
0
votes
0
replies
68
views
News:
Epigenomics Data Analysis
Epigenomics
RNA-seq
ATAC-seq
Chip-seq
HI-C
18 hours ago by
Physalia-courses
★ 2.6k
4
votes
8
replies
363
views
does any licenses needed to make experiments?
offtopic
updated 16 hours ago by
Dunois
★ 2.9k • written 1 day ago by
suleyman
• 0
6
votes
5
replies
167
views
Strange overrepresented sequence
fastQC
adapter
rnaseq
18 hours ago by
georomano
• 0
13
votes
6
replies
6.1k
views
Add metadata based on tree structure
R
ggtree
updated 19 hours ago by
rsieber
▴ 10 • written 5.4 years ago by
Jack
▴ 50
0
votes
3
replies
130
views
Normalize RNAseq data on RT-dPCR or RT-qPCR
RNAseq
updated 21 hours ago by
ATpoint
87k • written 23 hours ago by
lagartija
▴ 160
0
votes
2
replies
143
views
Is there a way to import a large amount of regions of interest into IGV
sequencing
igv
genomics
ngs
updated 21 hours ago by
GenoMax
150k • written 1 day ago by
Mark
▴ 30
4
votes
9
replies
450
views
Question about an alignment
ces
sequencing
alignment
27 minutes ago by
barslmn
★ 2.4k
0
votes
2
replies
160
views
Arioc (read mapping) ref sequence length error
mapping
gpu
arioc
read
14 hours ago by
Michael
• 0
0
votes
0
replies
127
views
When I want to compare two groups at multiple time points in scRNA-seq datasets, the criteria for the genes to be analyzed
Seurat
scRNA-seq
1 day ago by
Apprentice
▴ 170
7
votes
6
replies
437
views
Heatmap problem in R
R
Heatmap
2 days ago by
ParastooA
▴ 10
3
votes
4
replies
287
views
Bacterial VCF file annotation using snpEff error
SnpEff
1 day ago by
1769mkc
★ 1.2k
0
votes
4
replies
256
views
Coverage depth using samtools
samtools
ngs
depth
sequencing
coverage
22 hours ago by
slzr_
• 0
0
votes
2
replies
224
views
MarkDuplicates RNASeq: A few samples look weird. What could be the cause?
qc
markduplicates
picard
rnaseq
updated 2 days ago by
GenoMax
150k • written 2 days ago by
Davor
• 0
0
votes
1
reply
183
views
Data Integrity (NCBI SRA and TCGA)
clinic
metadata
updated 2 days ago by
GenoMax
150k • written 2 days ago by
umarfaruksahin
• 0
0
votes
0
replies
774
views
concatenating 2 differently-imputed VCF files then filtering by imputation score to keep the higher imputation score imputed vaiant from duplicate v…
Imputation
filtration
score
duplicate
variants
2 days ago by
SalmaElShafie
• 0
0
votes
0
replies
131
views
loss of coverage using gargammel
gargammel.pl
ART
AdapterRemoval
2 days ago by
anna.utili
• 0
0
votes
1
reply
188
views
What is the stance on optical duplicates in RNASeq?
duplicates
rnaseq
optical-duplicates
updated 2 days ago by
GenoMax
150k • written 2 days ago by
Davor
• 0
2
votes
4
replies
325
views
How to rank genes for GSEA using edgeR-LRT results ?
DEG
edgeR
updated 23 hours ago by
dariober
15k • written 3 days ago by
Picasa
▴ 660
4
votes
3
replies
360
views
Using bulk RNA-seq DE results to perform PCA in single cell RNA-seq
Single-cell
scRNAseq
updated 18 hours ago by
jared.andrews07
★ 18k • written 4 days ago by
Oli
• 0
3
votes
1
reply
202
views
Access to dbSnp137 (GRCh37)
dbSnp137
GRCh38
dbSnp
updated 3 days ago by
Marcellie87
▴ 10 • written 3 days ago by
Steven
▴ 50
0
votes
0
replies
141
views
How to convert RAP-DB/MSU IDS to RefSeq/Entrez Gene IDS?
IRGSP
Entrez
RAP-DB
RefSeq
3 days ago by
ChanderKant (CK) Chaudhary
▴ 10
4
votes
4
replies
1.7k
views
Annotating single cell data automatically
single-cell
updated 3 days ago by
S.Ghazala
▴ 60 • written 11 months ago by
Gerard
▴ 10
0
votes
0
replies
162
views
Bulk searching UniProt
uniprot
3 days ago by
timothy.kirkwood
▴ 140
4
votes
0
replies
218
views
News:
Join the online/distributed nf-core Hackathon next week!
nf-core
nextflow
container
workflows
bioinformatics
3 days ago by
Matthias Zepper
5.1k
0
votes
3
replies
267
views
Non variants sites of the genome (gVCF file)
non
genome
variants
sites
gVCF
updated 9 hours ago by
LauferVA
4.6k • written 3 days ago by
heureuse
▴ 10
1
vote
1
reply
211
views
Choosing the "right" sequence as an input control
input
control
chipseq
updated 3 days ago by
ATpoint
87k • written 3 days ago by
mark.pekarsky
▴ 20
1
vote
2
replies
251
views
Unable to parse config file
nextflow
nf-core
Pulled
form
github
updated 2 days ago by
Phil Ewels
★ 1.4k • written 3 days ago by
JieQY
• 0
0
votes
0
replies
164
views
Seeking Guidance on Selecting Control ChIP Sequences for nf-core/chipseq Pipeline
SRR
nf-core
chipseq
3 days ago by
mark.pekarsky
▴ 20
0
votes
0
replies
155
views
Problem with GSVA output
GSVA
RNAseq
3 days ago by
a.stef.44
▴ 10
0
votes
2
replies
241
views
Using UMI-tools on Smart-seq3 RNA-Seq data
rna-seq
UMI
UMI-tools
smart-seq3
updated 3 days ago by
Ram
45k • written 3 days ago by
Agastya
▴ 10
1
vote
5
replies
392
views
Remove site with only missing data
Fasta
updated 3 days ago by
Pierre Lindenbaum
165k • written 4 days ago by
Leane
• 0
0
votes
3
replies
290
views
Problems with Seaborn plots in Python
python
sns
plot
updated 3 days ago by
Wayne
★ 2.1k • written 4 days ago by
egascon
▴ 60
4
votes
4
replies
416
views
Downloading full list of Homo sapiens genes
Genes
4 days ago by
nigussie.amu
• 0
0
votes
1
reply
238
views
How to normalise Nanopore mRNA sequencing data between two cell lines
Nanopore
RNAseq
Normalization
updated 23 hours ago by
lagartija
▴ 160 • written 4 days ago by
Mo
▴ 50
0
votes
0
replies
334
views
Can I combine segment results from Sequenza and cnvkit?
CNV
sequenza
cnvkit
4 days ago by
Ram
45k
2
votes
5
replies
4.2k
views
Python library for parsing bcftools stats file
bcftools
updated 4 days ago by
Pierre Lindenbaum
165k • written 7.6 years ago by
William
★ 5.3k
0
votes
0
replies
189
views
Manta SV (specially Translocation)
manta
Structural-Variant
updated 3 days ago by
Ram
45k • written 4 days ago by
Nai
▴ 50
0
votes
3
replies
475
views
Cytoscape COLOUR EDGES
Reulatory
Cytoscape
Networks
updated 4 days ago by
Scooter
▴ 280 • written 13 days ago by
SKY
▴ 60
120,813 results • Page
1 of 2417
Recent Votes
Reproducibility Crisis
Answer: Differences in DEG results using RSEM with and without the --strandness reverse
Comment: RepBase30.02.fasta.tar.gz
Answer: gtf file for canFam2 genome version
Answer: gtf file for canFam2 genome version
Answer: illumina: Adapter Confusion
Answer: does any licenses needed to make experiments?
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Comment: RepBase30.02.fasta.tar.gz
by
Mensur Dlakic
★ 29k
No. The first item on their download page says very clearly: > You agree NOT to make the Repbase (or any part thereof, including Repbase R…
Comment: gtf file for canFam2 genome version
by
1769mkc
★ 1.2k
will give it a try thank you so much
Answer: CIBERSORTx error (error in rep(2, size * length(cells) -1)) : invalid 'times' ar
by
Andrew
• 0
I was just troubleshooting this error. It happens when you (most likely) tried to use averages for each cell type instead of replicates. Yo…
Answer: Differences in DEG results using RSEM with and without the --strandness reverse
by
LauferVA
4.6k
Emy - Im glad you asked this because you have to get this right for the results to be valid. Below is a breakdown of what the flags mean, a…
Answer: Absolute vs. differential gene expression - which analysis is more informative?
by
LauferVA
4.6k
One tends to use **absolute expression metrics** for quality control and to guarantee statistical power - i.e., to ensure that only genes w…
Comment: Non variants sites of the genome (gVCF file)
by
LauferVA
4.6k
if you are forced to use gVCF, this will be problematic. OK, you must use gVCF. additionally, is it mandatory that they all be in one fil…
Comment: making psam file for plink2
by
chrchang523
11k
https://www.cog-genomics.org/plink/2.0/formats#psam
Answer: making psam file for plink2
by
dec986
▴ 380
SOLUTION, ironically, from Grok: ----- Example .psam File Here’s an example of a simple .psam file with just the required column: …
Answer: gtf file for canFam2 genome version
by
Pierre Lindenbaum
165k
I wrote https://jvarkit.readthedocs.io/en/latest/KgToGff/ It was just a one-shot, I don't have used it much. Please check the results.…
Comment: Arioc (read mapping) ref sequence length error
by
Michael
• 0
Thanks for looking into that! Yes, the aligner does indeed calculate the lengths. FYI, the .cfg file I posted above was produced by the…
Comment: Question about an alignment
by
swbarnes2
14k
Okay, it's not throwing it away, it's making the supplementary alignment 106 bases away, instead of counting it as a single alignment with …
Comment: gtf file for canFam2 genome version
by
1769mkc
★ 1.2k
yes I'm looking for this canFam2 only that for some specific cases I have to use, even though I have the newer version also
Answer: Bioinformatics
by
Mensur Dlakic
★ 29k
STRING works with derived data, which means they don't do any analyses on their own. You may get some information by clicking on links with…
Comment: Absolute vs. differential gene expression - which analysis is more informative?
by
Mensur Dlakic
★ 29k
I don't think you will find a single person on this forum who will advocate for absolute expression analysis. In fact, almost the same ques…
Comment: Reproducibility Crisis
by
Mensur Dlakic
★ 29k
This pretty much covers all the common reasons. I will add an anecdote to further illustrate the difficulty. Not too long ago I was trainin…
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