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121,037 results • Page
1 of 2421
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2
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pangenome - Create a diagram venn
roary
grep
awk
pangenome
venn
updated 2 hours ago by
mplace
▴ 40 • written 2.0 years ago by
BATMAN
• 0
2
votes
3
replies
56
views
Addressing two nuisance factors in RNAseq
bulkRNAseq
updated 2 hours ago by
ATpoint
87k • written 2 hours ago by
jkim
▴ 190
0
votes
5
replies
147
views
DRAGEN FILTER
dragen
variant-calling
updated 5 hours ago by
GenoMax
150k • written 7 hours ago by
theresia.celine
• 0
836
votes
167
replies
174k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 11 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
1
vote
0
replies
80
views
Interpretation of the results: PCA
PCA
updated 5 hours ago by
Ram
45k • written 8 hours ago by
Ariadna
▴ 30
3
votes
5
replies
172
views
How to check normal BAM_files before create a Panel Of Normals (PoN)
GATK
variant_calling
PoN
7 hours ago by
AIMAR
• 0
0
votes
0
replies
58
views
News:
Phylogenetic Comparative Methods in R (12–16 May, Online)
R
Phylogenetic-Comparative-Methods
10 hours ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
126
views
how to handle infinite M-values in methylation array data
M-value
methylation
infinite
array
updated 13 hours ago by
Basti
★ 2.1k • written 1 day ago by
matt192
• 0
1
vote
3
replies
1.7k
views
Free and redistributable genomic data sets?
data
software-development
genomics
updated 15 hours ago by
Meeru
• 0 • written 9.2 years ago by
woemler
▴ 170
1
vote
5
replies
1.0k
views
Rna-seq data
data
rna-seq
updated 9 hours ago by
GenoMax
150k • written 3.1 years ago by
biology_inform
▴ 50
6
votes
3
replies
872
views
RNAseq example data
expression
RNA-Seq
data
next-gen
updated 17 hours ago by
ehaag
▴ 20 • written 4.6 years ago by
rakinitopo
▴ 10
0
votes
1
reply
232
views
Transcript_id with "-0" after "flair collapse"
flair
updated 9 hours ago by
GenoMax
150k • written 5 months ago by
anna_shin
• 0
0
votes
0
replies
76
views
Can I use glucose measured using two different devices in my study?
normalization
standardization
transformation
22 hours ago by
Faith
▴ 50
0
votes
2
replies
150
views
Limma model gives weird results
model
multivariate
Limma
updated 7 hours ago by
ATpoint
87k • written 1 day ago by
leranwangcs
▴ 150
1
vote
3
replies
621
views
Snakemake - pipeline shut down without error
snakemake
1 day ago by
bhumm
▴ 200
2
votes
2
replies
156
views
Source for `transcript_name` in GTFs
GTF
updated 8 hours ago by
Jorge - Ensembl
▴ 20 • written 1 day ago by
gernophil
▴ 120
5
votes
5
replies
268
views
Tool:
Lightweight bash pipeline for ddRADseq read pre-processing, demultiplexing, and de-duplication using cutadapt
rad-seq
ddRADseq
demultiplexing
deduplication
1 day ago by
Rafal
▴ 10
0
votes
2
replies
131
views
How to normalised DNA sequencing depth for genomic variant discovery ?
normalisation
read-depth
variant-calling
updated 1 day ago by
Ram
45k • written 1 day ago by
AIMAR
• 0
2
votes
4
replies
224
views
RNASeq featureCounts Help Needed
fungi
featurecounts
RNASeq
1 day ago by
SomeOne
▴ 170
0
votes
0
replies
90
views
PAUP* error with exporting result file
PAUP
SVDquartet
1 day ago by
ylkim9
• 0
0
votes
1
reply
114
views
I need to convert Axiom output to PLINK format I did not find annotation.db
Axiom
PLINK
apt-format-result
updated 1 day ago by
GenoMax
150k • written 1 day ago by
AlmuhayyaA
• 0
1
vote
3
replies
213
views
How to merge heterogeneously normalized RNA-seq datasets for meta-analysis?
normalization
rna-seq
updated 13 hours ago by
ATpoint
87k • written 1 day ago by
pipelinfections
• 0
1
vote
0
replies
120
views
Tool:
I packaged some tools in python pypi for Windows (fastqc, bowtie2, samtools)
python
pypi
17 hours ago by
Cris
▴ 10
0
votes
3
replies
282
views
Transcript ID
id
transcript
updated 1 day ago by
Meeru
• 0 • written 2 days ago by
cheong
• 0
0
votes
1
reply
153
views
Galaxy + RStudio
R
RNA-seq
RStudio
updated 1 day ago by
swbarnes2
14k • written 1 day ago by
justus
• 0
8
votes
18
replies
3.1k
views
8 follow
How to limit fasta header to 40 characters?
unix
seqkit
fasta
updated 1 day ago by
jena
▴ 320 • written 19 months ago by
fishingline37
• 0
1
vote
2
replies
212
views
Transcript level quantification and gene length scaling for rRNA depleted libraries
RNASeq
updated 2 days ago by
dsull
★ 7.4k • written 2 days ago by
marc.zimmerli
▴ 10
0
votes
0
replies
126
views
Model diagnostics for GAM (Generalised Additive Model)
gam
mgcv
generalized-additive-model
2 days ago by
Tuấn Anh
• 0
2
votes
5
replies
607
views
Forum:
Small‑Lab Data Management & Analytics Tool – What are your biggest pain points?
biotech
startup
updated 2 days ago by
Darked89
4.7k • written 5 days ago by
Novoo
▴ 10
1
vote
1
reply
184
views
landscape of RNA types and their functions/ scRNA
transcriptomics
scRNA
biology
updated 2 days ago by
ATpoint
87k • written 2 days ago by
Ariadna
▴ 30
1
vote
2
replies
210
views
Is vg toolkit suitable for haplotype calling?
pangenome
haplotype
vg
12 hours ago by
Pau
• 0
0
votes
0
replies
121
views
Dfam annotations
dfam
2 days ago by
frarodmar17
• 0
0
votes
3
replies
393
views
miRNA alignment and count generation
aligment
Mapping
Bowtie
miRNAs
updated 2 days ago by
i.sudbery
21k • written 5 days ago by
omicon
▴ 40
1
vote
2
replies
226
views
Cytoscape Node Size Mapping
Cytoscape
3 days ago by
William
▴ 20
1
vote
2
replies
271
views
Deeptools: Custom gcbias plots in R possible?
computegcbias
deeptools
3 days ago by
RJDan
• 0
0
votes
3
replies
816
views
HOMER Motif Analysis-- homer2 error
rna-seq
homer
updated 3 days ago by
ATpoint
87k • written 15 months ago by
HypoGG
• 0
4
votes
0
replies
208
views
Herald:
The Biostar Herald for Monday, April 21, 2025
herald
3 days ago by
Biostar
3.5k
0
votes
4
replies
286
views
Forum:
ELISA‑Focused Lab Management & Analysis App – Would You Use It?
biotech
startup
updated 3 days ago by
Ram
45k • written 3 days ago by
Novoo
▴ 10
0
votes
0
replies
229
views
Job:
Research Fellow in Cancer Evolution (Deadline 30 Apr 2025)
Cancer-Phylogenetics
Chromosomal-Instability
updated 2 days ago by
Ram
45k • written 3 days ago by
Bingxin
• 0
1
vote
10
replies
942
views
Proper preprocessing for ML after limma, quantile normalization and log2 transformation: Is standardization still necessary?
featureselection
microarray
preprocessing
updated 1 day ago by
Mensur Dlakic
★ 29k • written 5 days ago by
Sib
▴ 60
0
votes
0
replies
220
views
How to get gene count and transcript count from Stringtie
Stringtie
updated 4 days ago by
GenoMax
150k • written 4 days ago by
Abieskawa
• 0
1
vote
1
reply
360
views
Any recommendation for calculating Fu and Li's D using RStudio?
PopGenome
updated 3 days ago by
Michael
55k • written 6 days ago by
Chatchapon
• 0
1
vote
4
replies
455
views
Running out of disc space with wsl2
Memory
WSL2
capacity
Disc
updated 4 days ago by
Mensur Dlakic
★ 29k • written 5 days ago by
vladimir_vinarsky
• 0
0
votes
2
replies
311
views
Filtering vcf file for variants - including non-selected variants
bedtools
calling
variant
bcftools
5 days ago by
andrebolerbarros
• 0
0
votes
4
replies
462
views
UCSC's NCBI RefSeq Track tables: header differences
ucsc-genome-browswer
refseq
ncbi
6 days ago by
Synanth
• 0
3
votes
2
replies
1.4k
views
How do you do differential splicing using edgeR command diffSpliceDGE?
differential-splicing
diffSpliceDGE
edger
updated 5 days ago by
Gordon Smyth
★ 7.9k • written 3.2 years ago by
Assa Yeroslaviz
★ 1.9k
0
votes
0
replies
275
views
sequenced noise elimination in searching for snp
snp
sequencing
virus
6 days ago by
gagi1993
• 0
0
votes
1
reply
335
views
Issue with Agilent 2100 BioAnalyzer HS DNA Assay Trace
library-preparation
DNA
bioanalyzer
updated 6 days ago by
GenoMax
150k • written 7 days ago by
Beatrice
• 0
1
vote
2
replies
856
views
QIAseq 16S/ITS Screening Panel Primers?
16s
primers
metagenomics
ITS
updated 7 days ago by
GenoMax
150k • written 3.2 years ago by
Isaac
• 0
3
votes
2
replies
458
views
Analysing gene CNV from TCGA using TCGAbiolinks
TCGA
2 days ago by
Matt
• 0
121,037 results • Page
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Recent Votes
Comment: Addressing two nuisance factors in RNAseq
Answer: Addressing two nuisance factors in RNAseq
What exactly should and shouldn't go in Combat's model matrix?
What exactly should and shouldn't go in Combat's model matrix?
How to compute t-statistic
Interpretation of the results: PCA
C: bed to bigBed conversion
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Comment: Addressing two nuisance factors in RNAseq
by
jared.andrews07
★ 18k
Yes, that is what they're suggesting, as it is the simplest model for what you want (and makes the `RNA_extraction_day` and `library_prep_d…
Answer: pangenome - Create a diagram venn
by
mplace
▴ 40
I used supervenn, makes a nice easy to read venn alternative "https://github.com/gecko984/supervenn"
Comment: Addressing two nuisance factors in RNAseq
by
jkim
▴ 190
Thanks, ATpoint. But I'm not sure if I understand your suggestion. Are you suggesting the following design? ``` | sample_name | treatment …
Answer: Addressing two nuisance factors in RNAseq
by
ATpoint
87k
You have an extraction core? That's awesome. Anyway, why not keeping it as a single blocking factor. The samples extracted as A get preppe…
Comment: DRAGEN FILTER
by
GenoMax
150k
Equivalence of commands in the two modes is discussed in the first link above. Did you check that?
Comment: DRAGEN FILTER
by
theresia.celine
• 0
Hi, thank you for the information, I already obtained my VCF file with the default filter, but I'm questioning the reliability of this defa…
Comment: DRAGEN FILTER
by
theresia.celine
• 0
Hi, I'm using DRAGEN 4.2.7, which perform DRAGEN-ML, that's why the default filter is QUAL > 3. But I'm still wondering if this threshold i…
Comment: DRAGEN FILTER
by
DBScan
▴ 480
Which DRAGEN version are you using? The filtering heavily depends on which DRAGEN version and commands you have used.
Comment: DRAGEN FILTER
by
GenoMax
150k
DRAGEN uses an optimized version of GATK. See --> https://gatk.broadinstitute.org/hc/en-us/articles/4410456501915-Functional-equivalence-in…
Comment: How to check normal BAM_files before create a Panel Of Normals (PoN)
by
AIMAR
• 0
I've 12 normal samples. Thanks for the share
Comment: How to check normal BAM_files before create a Panel Of Normals (PoN)
by
AIMAR
• 0
Understood sir.
Comment: Limma model gives weird results
by
ATpoint
87k
> However I got 0 significant contig. That alone does not necessarily indicate a problem. There could in reality be non differences, or no…
Answer: Source for `transcript_name` in GTFs
by
Jorge - Ensembl
▴ 20
Hi! [Transcript naming][1] is part of the [Ensembl gene annotation pipeline][2]. There will be further review for species [annotated both b…
Comment: How to check normal BAM_files before create a Panel Of Normals (PoN)
by
GenoMax
150k
Do you have enough samples to create a private PON? GATK recommends a minimum of 40. If not you can use public 1000 genomes PON. https://g…
Comment: Rna-seq data
by
GenoMax
150k
You have to keep in mind that the portal you link only aggregates datasets for : **on Infectious and Immune-mediated Diseases (IID)**
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