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121,292 results • Page
1 of 2426
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Rank
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Votes
Replies
1
vote
3
replies
125
views
Use taxdb tp get scientific names in local BLAST output
taxdb
sscinames
taxonomy
makeblastdb
blast
31 minutes ago by
Luca Arbore
▴ 10
0
votes
0
replies
11
views
Arecthere tools to find differences in biochemical pathways between organisms?
bioinformatics
biochemistry
pathways
differential
25 minutes ago by
marongiu.luigi
▴ 740
0
votes
0
replies
14
views
How to automate blasting contigs?
assembly
contig
blast
WGS
36 minutes ago by
marongiu.luigi
▴ 740
0
votes
0
replies
17
views
DiffBind plot.profile error
DiffBind
BiocParallel
1 hour ago by
this_is_me_trying
• 0
0
votes
1
reply
32
views
Server Requirements for long read data analysis
longreadserver
longreadanalysis
serverrequirements
Server
updated 1 hour ago by
Michael
55k • written 1 hour ago by
genomics_student
• 0
0
votes
3
replies
635
views
News:
9th Berlin Summer School in NGS Data Analysis 2025 (June 30 - July 4, 2025) -- FINAL CALL --
Workshop
RNA-Seq
transcriptomics
VariantCalling
DNA-Seq
2 hours ago by
ecSeq Bioinformatics
▴ 20
837
votes
170
replies
178k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 5 weeks ago by
Biostar
3.6k • written 8.5 years ago by
Istvan Albert
102k
0
votes
3
replies
336
views
Random seed in scanpy
scanpy
scRNAseq
single-cell
updated 2 hours ago by
ATpoint
88k • written 5 days ago by
bioinfo
▴ 160
0
votes
1
reply
223
views
How to obtain the nodes in the graph corresponding to the variant in VCF
vg
updated 2 hours ago by
agis.maulana12
• 0 • written 4 days ago by
Wenhai
• 0
3
votes
4
replies
150
views
miRNA Quantifiaction Tool
quatification
mirna
10 hours ago by
anthony.santana.703.j
▴ 30
0
votes
1
reply
115
views
WGCNA pickSoftThreshold problem
picksoftthreshold
question
problem
wgcna
updated 14 hours ago by
LChart
4.9k • written 1 day ago by
ton_of_questions
• 0
2
votes
2
replies
410
views
plotProfile Errors of DiffBind
DiffBind
updated 14 hours ago by
this_is_me_trying
• 0 • written 3 months ago by
lijiangres
▴ 20
6
votes
6
replies
238
views
Fungal Annotation Comparison
Annotation
RNAseq
Fungus
genome_assembly
funannotate
updated 19 hours ago by
colindaven
7.5k • written 1 day ago by
SomeOne
▴ 170
0
votes
1
reply
192
views
Is comparing seeds sufficient, or should alignments be compared instead?
readmapping
updated 1 day ago by
shenwei356
8.7k • written 1 day ago by
curiousfellow@123
• 0
7
votes
2
replies
390
views
How to download protozoa reference genomes from NCBI
genomes
ncbi
updated 1 day ago by
colindaven
7.5k • written 7 days ago by
anna
▴ 70
2
votes
1
reply
195
views
Clustering on Big data (100 million samples)
Clustering
Data
Learning
Big
Machine
updated 1 day ago by
Mensur Dlakic
★ 29k • written 2 days ago by
Rushikesh
▴ 10
7
votes
6
replies
2.1k
views
Where can I find a vcf file with individuals in it, that have a specific disease?
Phenotype
VCF
updated 1 day ago by
ehaag
▴ 90 • written 17 months ago by
Patrick
▴ 30
0
votes
2
replies
488
views
ERROR::INVALID_TAG_NM:Record A, Read name B, NM tag (nucleotide differences) in file [0] does not match reality [1]
bwa
12 hours ago by
curious
▴ 890
5
votes
9
replies
637
views
Cut&Run TF Analysis - Very Low Peak Counts Despite Good Library Quality
macs2
cutandrun
chipseq
updated 7 days ago by
ATpoint
88k • written 7 days ago by
sk
▴ 20
0
votes
0
replies
116
views
Job:
Ensembl Genebuild Project Leader, EMBL-EBI, Hinxton near Cambridge, UK
Ensembl
jobs
updated 1 day ago by
GenoMax
151k • written 1 day ago by
A@Ensembl
▴ 30
0
votes
2
replies
167
views
tools to increase hi-c resolution
resolution
hi-c
1 day ago by
J.F.Jiang
▴ 930
2
votes
2
replies
171
views
How to fix this this tangle in my bacterial genome assembly
misassembly
assembly
genomics
ngs
2 hours ago by
Mark
▴ 50
5
votes
2
replies
355
views
Phased haplotype using WGS data from Nebula Genomics and GATK
phased
nebula
haplotype
wgs
gatk
updated 1 day ago by
colindaven
7.5k • written 6 days ago by
biostars
▴ 30
0
votes
4
replies
235
views
Help configuring RepeatMasker
human
illumina
libraries
RepeatMasker
updated 1 day ago by
Michael
55k • written 1 day ago by
María José
▴ 10
0
votes
2
replies
163
views
How to process Iso-Seq files in SRA public fastqs
Iso-seq
7 hours ago by
Abieskawa
• 0
14
votes
19
replies
8.2k
views
10 follow
ACMG assignment tools
wes
acmg
WES
WGS
updated 2 days ago by
Prash
▴ 290 • written 5.0 years ago by
Eugene A
▴ 190
2
votes
0
replies
175
views
EggNOG-mapper problem
all
Hi
updated 2 days ago by
GenoMax
151k • written 2 days ago by
Salma
▴ 20
0
votes
0
replies
137
views
Colsep for dendrogram and ColSideColors in heatmap.2
Heatmap
DGE
EdgeR
heatmap.2
2 days ago by
fabian
▴ 10
1
vote
1
reply
213
views
Decision making on choosing the based on tblastn result
interpretation
tblastn
BLAST
E-score
updated 3 days ago by
GenoMax
151k • written 3 days ago by
srestha
• 0
9
votes
7
replies
3.0k
views
How to Identify the pathogenicity of a set of variants (by using ACMG guidelines)?
pathogenicity
SNP
variants
updated 4 days ago by
Damianos P. Melidis
▴ 60 • written 6.5 years ago by
DanielC
▴ 210
0
votes
4
replies
486
views
Why am I getting the exact same MarkDuplicates results when passing a query or coordinate sorted bam?
picard
bwa
updated 1 day ago by
GenoMax
151k • written 4 days ago by
curious
▴ 890
0
votes
2
replies
293
views
use AverageExpression to stratify samples
scRNA
1 day ago by
ndra1456
• 0
0
votes
0
replies
852
views
Multiple taxonomy hits per OTU ID in VSEARCH despite --top_hits_only (90% ID after QIIME2 clustering at 97%)
vsearch
updated 4 days ago by
GenoMax
151k • written 4 days ago by
salma.sarker
• 0
0
votes
0
replies
250
views
RepeatMasker output file question
RepeatMasker
5 days ago by
xinguok794
• 0
5
votes
1
reply
548
views
Which GWAS tool to use that can work on Czech cases and British controls, on a binary trait?
plink2
gwas
saige
updated 5 days ago by
LChart
4.9k • written 6 days ago by
anita.szabo08
▴ 10
1
vote
2
replies
364
views
Unsure whether or not I overcorrected for batch effect in dataset
Quality
Plot
PCA
Control
updated 4 days ago by
GenoMax
151k • written 5 days ago by
Megan
▴ 60
0
votes
3
replies
367
views
Primer removal in amplicon sequencing
removal
Primer
Amplicon
DADA2
sequencing
updated 4 days ago by
GenoMax
151k • written 5 days ago by
sxf520
• 0
0
votes
1
reply
366
views
GATK BQSR error — Reference and BAM file chromosome name mismatch (“chr” vs. no “chr”)
GATK
VCF
BSQR
updated 5 days ago by
Ram
45k • written 5 days ago by
iamsmor
• 0
0
votes
1
reply
313
views
Batch correction without biological covariates
Combat-seq
RNA-seq
batch-correction
updated 5 days ago by
Ram
45k • written 5 days ago by
as823jk
• 0
2
votes
3
replies
375
views
RagTag results
NGS
scaffolding
RagTag
gaps
updated 5 days ago by
shelkmike
★ 1.6k • written 6 days ago by
synthiiihuh
• 0
0
votes
2
replies
352
views
Tools that output positions of matching kmers
sequences
genomics
kmers
1 day ago by
gil.hornung
▴ 100
2
votes
4
replies
428
views
mitochondrial assamply use NOVOplasty
mitochondrial
genome
mammles
updated 6 days ago by
cfos4698
★ 1.1k • written 6 days ago by
m90
▴ 30
0
votes
2
replies
334
views
Still bad phred score after fastp
phred
fastp
updated 5 days ago by
Ram
45k • written 6 days ago by
Shazel
• 0
0
votes
1
reply
302
views
Cytohubba problem and difference networks
Network
cytoscape
cytohubba
analysis
updated 6 days ago by
Scooter
▴ 310 • written 6 days ago by
mastorehpournazari
• 0
0
votes
1
reply
485
views
Cytoscape KEGG plots reverting to original layout after changing style or column
kegg
cytoscape
updated 6 days ago by
Scooter
▴ 310 • written 28 days ago by
Adrian
• 0
2
votes
4
replies
487
views
Mass-spectrometry proteomics
Proteomics
analysis
updated 4 days ago by
Gordon Smyth
★ 8.1k • written 6 days ago by
Nasim Gandomdoust
• 0
8
votes
3
replies
445
views
Biological meaning of metrics commonly used for QC in scRNA-seq?
ribosome
QC
velocity
single-cell
intron
updated 5 days ago by
jared.andrews07
★ 18k • written 6 days ago by
txema.heredia
▴ 240
0
votes
0
replies
239
views
News:
Eukaryotic eDNA Metabarcoding Course (14–18 July, Online)
Metabarcoding
eDNA
Genomics
Nanopore
6 days ago by
Physalia-courses
★ 2.6k
7
votes
12
replies
804
views
miRNA low mapping(Qiagen miRNA Library Prep)
Qiagen
pipeline
trimming
alignment
miRNA
updated 6 days ago by
GenoMax
151k • written 9 days ago by
anthony.santana.703.j
▴ 30
0
votes
12
replies
1.1k
views
STAR alignment not detecting some transcripts / custom reference
star
bwa
rna-seq
alignment
scrna-seq
updated 6 days ago by
rfran010
★ 1.5k • written 11 days ago by
npont
• 0
121,292 results • Page
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Recent Votes
Answer: Where can I find a vcf file with individuals in it, that have a specific disease
Comment: miRNA Quantifiaction Tool
How to download protozoa reference genomes from NCBI
How to download protozoa reference genomes from NCBI
plotProfile Errors of DiffBind
plotProfile Errors of DiffBind
miRNA Quantifiaction Tool
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Recent Replies
Comment: Use taxdb tp get scientific names in local BLAST output
by
Luca Arbore
▴ 10
Thank you for replies. Fast headers of downloaded sequences look like this grep "^>" diptera_coi_030625.fasta | head -n 5 >AB0…
Answer: Server Requirements for long read data analysis
by
Michael
55k
Like always, it depends on what you want to do and at what scale. The most resource-intensive step determines the minimum requirements of t…
Answer: 9th Berlin Summer School in NGS Data Analysis 2025 (June 30 - July 4, 2025) -- S
by
ecSeq Bioinformatics
▴ 20
Good news! Due to the high demand, we have enlarged the room and extended the registration deadline to 20 June!
Answer: Random seed in scanpy
by
ATpoint
88k
Generally, you need fixed seeds if you want to make analysis reproducible that has a random element. I cannot speak for ScanPy and Python, …
Comment: How to fix this this tangle in my bacterial genome assembly
by
Mark
▴ 50
I should have mentioned that the assembly was performed with only nanopore reads. I was just wondering if anything could be done through ma…
Answer: How to obtain the nodes in the graph corresponding to the variant in VCF
by
agis.maulana12
• 0
Straightforward way is using `vg deconstruct -a {vg_graph.gbz} > {variants_in_graph.vcf}`. The variants can be refered back to the input vc…
Comment: How to process Iso-Seq files in SRA public fastqs
by
Abieskawa
• 0
No, the files that it generated were all empty. I did use "top" to check if there is any program use large amount of resources, but I did n…
Comment: miRNA Quantifiaction Tool
by
anthony.santana.703.j
▴ 30
I undertand, its the whole "Ive only seen as far as i can because ive stood on the shoulder of giants", I just want familiarity with tools …
Comment: miRNA Quantifiaction Tool
by
GenoMax
151k
> You also limit yourself to the constraints of their pipeline, theres also no harm in building my own. Absolutely. Consider this though. …
Comment: miRNA Quantifiaction Tool
by
anthony.santana.703.j
▴ 30
No yes, I was satsified just wondering if the tool is sufficient enough. I even got alignment and everything. I know no tool is perfect whi…
Comment: miRNA Quantifiaction Tool
by
GenoMax
151k
> Im working on developing my own pipeline Is there a specific reason you want to do that? Even if you are learning the process, there a…
Comment: ERROR::INVALID_TAG_NM:Record A, Read name B, NM tag (nucleotide differences) in
by
curious
▴ 890
Thanks for the response I think I am more wondering if this is a typical thing that people see and am wondering what is wrong with my data…
Answer: WGCNA pickSoftThreshold problem
by
LChart
4.9k
2.5K genes sounds to me like over-filtering; and what was your rationale for removing variable (high CV) genes? My suggestion would be to o…
Comment: plotProfile Errors of DiffBind
by
this_is_me_trying
• 0
Hello, I'm having the same problem. Did you find a solution?
Comment: Cut&Run TF Analysis - Very Low Peak Counts Despite Good Library Quality
by
ATpoint
88k
There is mostly noise and no clear signal. It suggests that, as said before, IP was bad.
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