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Recent Replies
Comment: C.reinhardtii single-cell Seurat object - gene names instead of loci
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Thank you for your answer!
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It is always safer to get sequence/annotations from standard sources like NCBI/Ensembl so no manipulations are required to make the data wo…
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Moved this to an answer since it seems to be a logical explanation for the observation reported in original post.
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Looking at the size distribution the data must already be pre-trimmed so it does not make sense to trim again. You should merge data from t…
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I think it just counts the total number of lines while there could be multiple adapters types per sample (ex : illumina_universal_adapter, …
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I got it figured out, thanks!
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Since only tools available (confirmed by @colindaven ) require illumina reads you would be basically creating "illumina like" reads from yo…
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It showed me the same positions as that of PrimerBLAST.
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