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121,046 results • Page
1 of 2421
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News:
Genome Bioinformatics Summer School: OMICSS-25
training
bioinformatics
school
53 minutes ago by
Lilit
• 0
0
votes
1
reply
25
views
Unable to find mitochondrial genes in latest rat genome(GRCr8)
seurat
genome
mitochondrialgenes
NCBI
Singlecell
updated 54 minutes ago by
GenoMax
150k • written 1 hour ago by
dxj294
• 0
0
votes
0
replies
12
views
Haplotype reference for ShapeIt (ShapeIt5)
Phasing
1 hour ago by
evan.kransdorf
• 0
5
votes
5
replies
66
views
Pre-Made STAR Index for mm10 Mouse Genome
STAR
1 hour ago by
Nerelys
• 0
1
vote
1
reply
73
views
The volcano plot shows data with an arm-shaped enrichment.
volcanoplot
updated 3 hours ago by
ATpoint
87k • written 3 hours ago by
lea.preuss
• 0
0
votes
0
replies
52
views
Tool:
GENetLib: A Python Library for Gene–environment Interaction Analysis via Deep Learning
gene-environment
interaction
analysis
5 hours ago by
Barry
• 0
8
votes
11
replies
293
views
STAR vs. Kallisto
STAR
kallisto
alignment
RNA-Seq
mapping
updated 2 hours ago by
dsull
★ 7.4k • written 8 hours ago by
gogeni5529
▴ 60
0
votes
0
replies
38
views
How to Annotate VCF with AlphaMissense Using VEP and dbNSFP v5.1a in Offline Mode?
AlphaMissense
dbNSFP
VEP
ensembl
7 hours ago by
DareDevil
★ 4.4k
0
votes
1
reply
127
views
News:
Phylogenetic Comparative Methods in R (12–16 May, Online)
R
Phylogenetic-Comparative-Methods
updated 10 hours ago by
Meeru
• 0 • written 1 day ago by
Physalia-courses
★ 2.6k
836
votes
167
replies
174k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 12 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
0
votes
1
reply
105
views
Biostar handbook
Handbook
Biostar
updated 17 hours ago by
GenoMax
150k • written 17 hours ago by
himanshu0102
• 0
2
votes
2
replies
1.3k
views
pangenome - Create a diagram venn
roary
grep
awk
pangenome
venn
updated 21 hours ago by
mplace
▴ 40 • written 2.0 years ago by
BATMAN
• 0
4
votes
4
replies
213
views
Addressing two nuisance factors in RNAseq
bulkRNAseq
updated 8 hours ago by
ATpoint
87k • written 21 hours ago by
jkim
▴ 190
0
votes
5
replies
238
views
DRAGEN FILTER
dragen
variant-calling
updated 1 day ago by
GenoMax
150k • written 1 day ago by
theresia.celine
• 0
1
vote
0
replies
127
views
Interpretation of the results: PCA
proteomics
PCA
1 hour ago by
Ariadna
▴ 30
3
votes
5
replies
250
views
How to check normal BAM_files before create a Panel Of Normals (PoN)
GATK
variant_calling
PoN
1 day ago by
AIMAR
• 0
0
votes
1
reply
156
views
how to handle infinite M-values in methylation array data
M-value
methylation
infinite
array
updated 1 day ago by
Basti
★ 2.1k • written 1 day ago by
matt192
• 0
1
vote
3
replies
1.7k
views
Free and redistributable genomic data sets?
data
software-development
genomics
updated 1 day ago by
Meeru
• 0 • written 9.2 years ago by
woemler
▴ 170
1
vote
5
replies
1.0k
views
Rna-seq data
data
rna-seq
updated 1 day ago by
GenoMax
150k • written 3.1 years ago by
biology_inform
▴ 50
6
votes
3
replies
905
views
RNAseq example data
expression
RNA-Seq
data
next-gen
updated 1 day ago by
ehaag
▴ 20 • written 4.6 years ago by
rakinitopo
▴ 10
0
votes
1
reply
260
views
Transcript_id with "-0" after "flair collapse"
flair
updated 1 day ago by
GenoMax
150k • written 5 months ago by
anna_shin
• 0
0
votes
0
replies
103
views
Can I use glucose measured using two different devices in my study?
normalization
standardization
transformation
1 day ago by
Faith
▴ 50
0
votes
2
replies
198
views
Limma model gives weird results
model
multivariate
Limma
updated 1 day ago by
ATpoint
87k • written 1 day ago by
leranwangcs
▴ 150
1
vote
3
replies
650
views
Snakemake - pipeline shut down without error
snakemake
1 day ago by
bhumm
▴ 200
2
votes
2
replies
201
views
Source for `transcript_name` in GTFs
GTF
updated 1 day ago by
Jorge - Ensembl
▴ 20 • written 2 days ago by
gernophil
▴ 120
5
votes
5
replies
310
views
Tool:
Lightweight bash pipeline for ddRADseq read pre-processing, demultiplexing, and de-duplication using cutadapt
rad-seq
ddRADseq
demultiplexing
deduplication
2 days ago by
Rafal
▴ 10
0
votes
2
replies
164
views
How to normalised DNA sequencing depth for genomic variant discovery ?
normalisation
read-depth
variant-calling
updated 2 days ago by
Ram
45k • written 2 days ago by
AIMAR
• 0
2
votes
4
replies
258
views
RNASeq featureCounts Help Needed
fungi
featurecounts
RNASeq
2 days ago by
SomeOne
▴ 170
0
votes
0
replies
119
views
PAUP* error with exporting result file
PAUP
SVDquartet
2 days ago by
ylkim9
• 0
0
votes
1
reply
143
views
I need to convert Axiom output to PLINK format I did not find annotation.db
Axiom
PLINK
apt-format-result
updated 2 days ago by
GenoMax
150k • written 2 days ago by
AlmuhayyaA
• 0
1
vote
3
replies
256
views
How to merge heterogeneously normalized RNA-seq datasets for meta-analysis?
normalization
rna-seq
updated 1 day ago by
ATpoint
87k • written 2 days ago by
pipelinfections
• 0
1
vote
0
replies
154
views
Tool:
I packaged some tools in python pypi for Windows (fastqc, bowtie2, samtools)
python
pypi
1 day ago by
Cris
▴ 10
0
votes
3
replies
310
views
Transcript ID
id
transcript
updated 2 days ago by
Meeru
• 0 • written 3 days ago by
cheong
• 0
0
votes
1
reply
182
views
Galaxy + RStudio
R
RNA-seq
RStudio
updated 2 days ago by
swbarnes2
14k • written 2 days ago by
justus
• 0
8
votes
18
replies
3.1k
views
8 follow
How to limit fasta header to 40 characters?
unix
seqkit
fasta
updated 2 days ago by
jena
▴ 320 • written 19 months ago by
fishingline37
• 0
1
vote
2
replies
245
views
Transcript level quantification and gene length scaling for rRNA depleted libraries
RNASeq
updated 2 days ago by
dsull
★ 7.4k • written 2 days ago by
marc.zimmerli
▴ 10
0
votes
0
replies
155
views
Model diagnostics for GAM (Generalised Additive Model)
gam
mgcv
generalized-additive-model
3 days ago by
Tuấn Anh
• 0
2
votes
5
replies
645
views
Forum:
Small‑Lab Data Management & Analytics Tool – What are your biggest pain points?
biotech
startup
updated 3 days ago by
Darked89
4.7k • written 6 days ago by
Novoo
▴ 10
1
vote
1
reply
213
views
landscape of RNA types and their functions/ scRNA
transcriptomics
scRNA
biology
updated 3 days ago by
ATpoint
87k • written 3 days ago by
Ariadna
▴ 30
1
vote
4
replies
285
views
Is vg toolkit suitable for haplotype calling?
pangenome
haplotype
vg
40 minutes ago by
Pau
• 0
0
votes
0
replies
150
views
Dfam annotations
dfam
3 days ago by
frarodmar17
• 0
0
votes
3
replies
426
views
miRNA alignment and count generation
aligment
Mapping
Bowtie
miRNAs
updated 3 days ago by
i.sudbery
21k • written 6 days ago by
omicon
▴ 40
1
vote
2
replies
259
views
Cytoscape Node Size Mapping
Cytoscape
3 days ago by
William
▴ 20
1
vote
2
replies
305
views
Deeptools: Custom gcbias plots in R possible?
computegcbias
deeptools
3 days ago by
RJDan
• 0
0
votes
3
replies
847
views
HOMER Motif Analysis-- homer2 error
rna-seq
homer
updated 3 days ago by
ATpoint
87k • written 15 months ago by
HypoGG
• 0
4
votes
0
replies
239
views
Herald:
The Biostar Herald for Monday, April 21, 2025
herald
4 days ago by
Biostar
3.5k
0
votes
4
replies
318
views
Forum:
ELISA‑Focused Lab Management & Analysis App – Would You Use It?
biotech
startup
updated 3 days ago by
Ram
45k • written 4 days ago by
Novoo
▴ 10
0
votes
0
replies
258
views
Job:
Research Fellow in Cancer Evolution (Deadline 30 Apr 2025)
Cancer-Phylogenetics
Chromosomal-Instability
updated 2 days ago by
Ram
45k • written 4 days ago by
Bingxin
• 0
1
vote
10
replies
1.1k
views
Proper preprocessing for ML after limma, quantile normalization and log2 transformation: Is standardization still necessary?
featureselection
microarray
preprocessing
updated 2 days ago by
Mensur Dlakic
★ 29k • written 6 days ago by
Sib
▴ 60
0
votes
0
replies
246
views
How to get gene count and transcript count from Stringtie
Stringtie
updated 5 days ago by
GenoMax
150k • written 5 days ago by
Abieskawa
• 0
121,046 results • Page
1 of 2421
Recent Votes
C: Get gene length with R
Comment: Pre-Made STAR Index for mm10 Mouse Genome
Answer: Pre-Made STAR Index for mm10 Mouse Genome
Comment: Pre-Made STAR Index for mm10 Mouse Genome
Answer: Pre-Made STAR Index for mm10 Mouse Genome
Answer: STAR vs. Kallisto
Comment: STAR vs. Kallisto
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leranwangcs
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Ne
▴ 10
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dxj294
• 0
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▴ 450
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Recent Replies
Comment: Is vg toolkit suitable for haplotype calling?
by
Pau
• 0
Just to be more explicit: I am working with a polyploid monoexonic gene whose boundaries are well annotated. I have many cDNA ONT reads (fu…
Answer: Unable to find mitochondrial genes in latest rat genome(GRCr8)
by
GenoMax
150k
It appears that the mitochondrial genome has not made it to the `RefSeq` version of GRCr8. It is present in GenBank version though: https:/…
Comment: Pre-Made STAR Index for mm10 Mouse Genome
by
Nerelys
• 0
Yes, I will consider that also. Thanks a lot!
Comment: Pre-Made STAR Index for mm10 Mouse Genome
by
Nerelys
• 0
Thank you so much!
Comment: Pre-Made STAR Index for mm10 Mouse Genome
by
GenoMax
150k
> as I do not have sufficient resources to generate the index myself. If that is the case you may also run into issues with alignments. ST…
Comment: Pre-Made STAR Index for mm10 Mouse Genome
by
dsull
★ 7.4k
Darn it lol! Think you beat me by a second!
Answer: Pre-Made STAR Index for mm10 Mouse Genome
by
dsull
★ 7.4k
Found this online :) http://refgenomes.databio.org/v3/assets/splash/0f10d83b1050c08dd53189986f60970b92a315aa7a16a6f1/star_index?tag=defa…
Comment: STAR vs. Kallisto
by
dsull
★ 7.4k
Of course! Oh, the parameter is --d-list. By default, in `kb ref`, --d-list is set to whatever FASTA you supply to `kb ref` but you can dis…
Comment: STAR vs. Kallisto
by
gogeni5529
▴ 60
thanks @delaneyksull for the comprehensive responses both in the answer as well as in the comments. I appreciate the time. A comment to y…
Comment: STAR vs. Kallisto
by
dsull
★ 7.4k
Also, this isn't arguing for/against kallisto. You're mentioning situations about ALIGNMENT issues; this is a QUANTIFICATION issue.
Comment: STAR vs. Kallisto
by
dsull
★ 7.4k
A ton of benchmarking (across many papers) has already been done to show which tools perform best when. The literature has a ton of evidenc…
Comment: The volcano plot shows data with an arm-shaped enrichment.
by
ATpoint
87k
Usually odd shapes in these sorts of plots come from insufficient prefiltering. Here, in your `keep` all you do is to make sure that the ge…
Comment: STAR vs. Kallisto
by
ATpoint
87k
Unless you comprehensively show that one tool performs globally better and reveals more meaningful biology than the other the choice is in …
Comment: STAR vs. Kallisto
by
dsull
★ 7.4k
After re-reading this, I'm going to push back against this answer a bit. In this case, it's 100% clear that pseudoalignment is producing th…
Comment: Is there a tool to obtain GO terms for thousands of genes at once?
by
GenoMax
150k
GeneSCF may be an option: https://github.com/genescf/GeneSCF?tab=readme-ov-file#Step-by-step-instructions Please be aware that use of KEGG…
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