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121,130 results • Page
1 of 2423
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14
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Best Practices for Integrating RNA-seq Time Series from Multiple Studies with Varying Protocols
differential-expression
meta-analysis
integration
RNA-seq
time-series
1 hour ago by
enee
▴ 20
10
votes
9
replies
4.7k
views
bcftools query add header
bcftools
updated 1 hour ago by
GenoMax
151k • written 3.1 years ago by
User000
▴ 750
0
votes
1
reply
42
views
RNA-Sequencing Analysis when control samples paired-end and tumour samples single-end
bioinformatics
GEO
rna-sequencing
updated 2 hours ago by
GenoMax
151k • written 2 hours ago by
h
• 0
0
votes
2
replies
85
views
Nextflow: split a FASTA file into 5 parts to enable parallel processing
nextflow
workflow
pipeline
updated 2 hours ago by
GenoMax
151k • written 4 hours ago by
neng
• 0
0
votes
1
reply
121
views
Alternative to PASApipeline
transcriptomics
annotation
updated 6 hours ago by
lieven.sterck
15k • written 1 day ago by
lagartija
▴ 160
0
votes
0
replies
48
views
How to Get a Genetic Map, Given the Physical Position and Genotype Data of Markers
map
genetic
map.
physical
6 hours ago by
Daniyal
• 0
1
vote
7
replies
2.3k
views
vcftools --weir-fst-pop returns -nan
fst
vcftools
updated 8 hours ago by
Michael
55k • written 2.4 years ago by
elizabeth
• 0
0
votes
1
reply
97
views
HISAT2 with Cleaned Data
HISAT2
RNASeq
updated 5 hours ago by
colindaven
7.4k • written 12 hours ago by
Gordonz9494
• 0
837
votes
170
replies
175k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 10 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
0
votes
1
reply
111
views
Definitions for "differential expression" and "differential abundance" in RNA-seq?
rna-seq
abundance
expression
differential
deseq
updated 13 hours ago by
dsull
★ 7.5k • written 16 hours ago by
tw_140
• 0
0
votes
4
replies
203
views
Efficiently find regions in bed file that are within start and end regions of another bed file.
bedtools
intersect
ranges
genomic
updated 18 hours ago by
Pierre Lindenbaum
166k • written 23 hours ago by
rxs1018
• 0
2
votes
4
replies
161
views
16S Libraries With Non-Purified Short Fragments
Sequencing
16S
Libraries
Magnetic
Beads
2 hours ago by
alenew.am
▴ 10
0
votes
1
reply
112
views
How to map polyA tails to barcodes in paired end reads for single cell RNA-seq
RNA-seq
cell
polyA
single
updated 1 day ago by
GenoMax
151k • written 1 day ago by
Ana
▴ 10
7
votes
6
replies
4.8k
views
Questions related to ChromHMM+using MACS2 peak calling output bed file as input for ChromHMM?
macs2
ChIP-Seq
ChromHMM
MACS2
CHROMHMM
updated 1 day ago by
luminea
▴ 10 • written 5.9 years ago by
m.sadman.sakib
▴ 120
4
votes
5
replies
365
views
Editing and Adding to a GTF file
Annotation
Gene
GTF
ID
updated 1 day ago by
gernophil
▴ 120 • written 2 days ago by
Keem Uarren Eiryll
▴ 10
4
votes
2
replies
142
views
Averaging multiple IgG BAM files for use as a control in ChromHMM
ChromHMM
bam
averag
updated 1 day ago by
ATpoint
88k • written 1 day ago by
luminea
▴ 10
3
votes
3
replies
356
views
Deduplication rate of shotgun metagenomes using fastqc
metagenome
fastqc
shotgun
deduplication
updated 1 day ago by
colindaven
7.4k • written 6 days ago by
vikasmh111
• 0
0
votes
2
replies
143
views
differential exon usage from Salmon outputs
edgeR
diffSpliceDGE
DEU
salmon
1 day ago by
n_navy
• 0
0
votes
0
replies
85
views
News:
online course: Introduction to Genome Annotation
Gene-Prediction
Genome-Annotation
Gene-Models
1 day ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
425
views
ValueError: Found array with 0 sample(s) (shape=(0, 138)) while a minimum of 1 is required. In metawrap - concoct binning module
concoct
metawrap
module
1 day ago by
shevch2009
• 0
0
votes
7
replies
294
views
Circular heatmap using circos.heatmap in R
R
circos
heatmap
circularheatmap
updated 2 hours ago by
GenoMax
151k • written 1 day ago by
Yukta
• 0
0
votes
0
replies
110
views
Zebrafish phenotype ontology enrichment analyses
ontology
zebrafish
1 day ago by
Li
• 0
0
votes
2
replies
195
views
Sequence Duplication for Single-End 3RAD Data
duplication
reduced-representation
18 hours ago by
Sarah
• 0
0
votes
0
replies
111
views
Cytoscape KEGG plots reverting to original layout after changing style or column
kegg
cytoscape
1 day ago by
Adrian
• 0
1
vote
6
replies
1.0k
views
6 follow
Not retrieving Mitochondrial Genes in Single Cell analysis
single-cell
updated 1 day ago by
Ram
45k • written 10 months ago by
guioliveirareis
▴ 10
0
votes
0
replies
118
views
News:
Ancient Metagenomics course - online, May 27-29
ancient-metagenomics
aDNA
aMeta
2 days ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
154
views
Issues running scAPAtrap
scAPAtrap
polyA
scRNAseq
updated 2 days ago by
GenoMax
151k • written 2 days ago by
Ana
▴ 10
5
votes
3
replies
262
views
What are BED files?
BED
updated 1 day ago by
Alex Reynolds
36k • written 2 days ago by
Besoa
• 0
3
votes
8
replies
432
views
ggplot make boxplots more wide
ggplot2
r
2 days ago by
Nona
▴ 90
2
votes
1
reply
206
views
Minimum number of samples for multi-omics data integration
multi-omics
updated 1 day ago by
Ram
45k • written 2 days ago by
Tundup
▴ 10
1
vote
0
replies
152
views
News:
Workshop: Virtual Genome Annotation, May 27–29
GenomeAnnotation
workshop
UConn
2 days ago by
zsc25001
▴ 10
1
vote
1
reply
193
views
Assigning a string variable on Visual Studio using python
python
visual-studio
updated 1 day ago by
Ram
45k • written 2 days ago by
arthur_alexander83
• 0
4
votes
4
replies
319
views
Tool:
Gene Exons Painter
Genomics
Visualisation
Exons
Transcripts
Isoforms
updated 1 day ago by
benformatics
4.1k • written 2 days ago by
Rafal
▴ 70
0
votes
0
replies
152
views
News:
Course online:Functional programming in R, May 27-30
R
programming
functional
2 days ago by
soledad.esteban
• 0
3
votes
1
reply
230
views
Herald:
The Biostar Herald for Monday, May 05, 2025
herald
updated 2 days ago by
cmdcolin
★ 4.2k • written 3 days ago by
Biostar
3.5k
0
votes
0
replies
133
views
How to generate .AED files from .CEL files using Linux (Affymetrix CytoScan HT-CMA 96F)
HT-CMA-96
analysis
data
affymetrix
microarray
3 days ago by
genetixs
▴ 20
0
votes
0
replies
146
views
News:
Online course - Introduction to GWAS
R
GWAS
Statistics
3 days ago by
Physalia-courses
★ 2.6k
4
votes
3
replies
275
views
Sample level genotype mapping in plink vcf output
VCF
updated 2 days ago by
Pierre Lindenbaum
166k • written 3 days ago by
1769mkc
★ 1.3k
6
votes
2
replies
300
views
Tool:
RNAseq Power Calculator
calculator
Replicates
RNAseq
Statistics
Power
3 days ago by
Rafal
▴ 70
1
vote
2
replies
269
views
Association (Protein interaction) in Intact database results what can really mean?
protein-protein-interaction
database
protein
proteins
Intact
5 hours ago by
Bendegúz
• 0
1
vote
4
replies
417
views
About FastP Tool
Raw
Quality_control
Preprocessing
alignment
reads
2 days ago by
AIMAR
• 0
3
votes
9
replies
673
views
Transcript identification and quantification
splitseq
transcript
scRNA
1 day ago by
anton.avramov
• 0
0
votes
1
reply
266
views
Treatment of nanoparticles after buying before the experiment
fermentation
treatment
Nanoparticles
dark
updated 5 days ago by
ATpoint
88k • written 5 days ago by
Nick
• 0
2
votes
1
reply
271
views
Tool:
Accessible color palettes for charts and dataviz
dataviz
updated 3 days ago by
Bastien Hervé
6.2k • written 5 days ago by
Pierre
▴ 20
3
votes
4
replies
423
views
Warning in bcftools when subsetting a VCF by variant ID
bcftools
VCF
Beagle5.5
updated 2 days ago by
Pierre Lindenbaum
166k • written 5 days ago by
Mwangana
• 0
1
vote
2
replies
316
views
Lucidea Array Spotter - Question about machinery
sequencing
Microarray
updated 5 days ago by
GenoMax
151k • written 6 days ago by
Taylor
• 0
0
votes
3
replies
400
views
Differeces between Seurat::RunPCA and scater::runPCA affect downstream analyses
slingshot
PCA
Seurat
scater
single-cell
updated 6 days ago by
Bastien Hervé
6.2k • written 6 days ago by
txema.heredia
▴ 210
1
vote
1
reply
288
views
What is the difference between JASPAR IDs MA0668.3 and MA1993.2 for Neurod2?
motifs
atac-seq
factor
jaspar
transcription
updated 6 days ago by
GenoMax
151k • written 6 days ago by
Ambuj
• 0
0
votes
3
replies
379
views
How can I find KO IDs for ORF sequences in a large FASTA file?
KEGG
ORF
updated 5 days ago by
Ram
45k • written 6 days ago by
Nikesh
• 0
0
votes
0
replies
242
views
Single MAG Recruitment to multiple metaT Samples
metagenomics
rna-seq
transcriptomics
6 days ago by
zjhennin
• 0
121,130 results • Page
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Comment: bcftools query add header
Comment: 16S Libraries With Non-Purified Short Fragments
Answer: bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open
A: Getting haplotype specific FASTA files from VCF files
Answer: Why did my data get worse after trimming with trimmomatic?
Answer: Why did my data get worse after trimming with trimmomatic?
Comment: Trimmomatic - 2nd paired fastq is empty
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Comment: Single cell demultiplexing using 10x and TotalSeq-B
by
txema.heredia
▴ 210
> Did you find an answer? Unfortunately, no. > I was wondering if its better to use HTOdemux rather than the cellranger's version? I tri…
Answer: bcftools query add header
by
DBScan
▴ 480
In case somebody else stumbles upon this question, `-HH` disables the column numbers: ``` bcftools query -HH -f '%CHROM\t%POS\t%REF\t%AL…
Comment: Circular heatmap using circos.heatmap in R
by
GenoMax
151k
Please consider accepting the original answer (green check mark) to provide closure to this thread).
Comment: RNA-Sequencing Analysis when control samples paired-end and tumour samples singl
by
GenoMax
151k
> It seems to be that the control sample runs are paired end data with 2 fasta files whilst the 12 tumour samples are only single-end read…
Comment: Nextflow: split a FASTA file into 5 parts to enable parallel processing
by
GenoMax
151k
It may be useful to also indicate how you are running this code? Are you supplying a single fasta file name or five pieces? > [[a, a1], [b…
Comment: 16S Libraries With Non-Purified Short Fragments
by
alenew.am
▴ 10
I will do it, thank you!
Comment: 16S Libraries With Non-Purified Short Fragments
by
alenew.am
▴ 10
Ok! Thank you for the suggestions
Comment: Circular heatmap using circos.heatmap in R
by
Yukta
• 0
@Bastien Herve thank you for the help, I understood the gap.degree and the clustering method. The code worked for me with my data also.
Comment: Nextflow: split a FASTA file into 5 parts to enable parallel processing
by
lieven.sterck
15k
no clue on nextflow but do assure that the split files are of equal size, otherwise you might get little gain from the splitting it, as one…
Comment: Combining scRNA-seq datasets that have been processed differently
by
dthorbur
★ 3.0k
I would suggest only using these datasets for preliminary analyses. If you are trying to compare between cell types, any results you find w…
Comment: Circular heatmap using circos.heatmap in R
by
Bastien Hervé
6.2k
Let's say this is your original dataframe : vst_data = rbind(cbind(matrix(rnorm(50*5, mean = 1), nr = 50), mat…
Comment: 16S Libraries With Non-Purified Short Fragments
by
dthorbur
★ 3.0k
Also, please only [cross post](https://biology.stackexchange.com/questions/116463/16s-libraries-with-non-purified-short-fragments) when abs…
Comment: Association (Protein interaction) in Intact database results what can really mea
by
Bendegúz
• 0
Thanks a lot for the help and clarification. If one of the protein is a membranprotein and the other is protein has enzymefunction. Could …
Answer: Alternative to PASApipeline
by
lieven.sterck
15k
I'm not sure if there are any one-to-one replacements for PASA but there are for sure other tools that can do transcript alignment/reconstr…
Comment: HISTA2 with Cleaned Data
by
lieven.sterck
15k
You mention "counts are too low", why do you think so? can you put a number on it? Does the SBATCH script runs but times-out or is it stuc…
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